Year 2019, Volume 58 , Issue 3, Pages 295 - 302 2019-09-20

Effect of aspirin on protein levels of paraoxonase enzymes and arylesterase activity in human-derived hepatoma cells
Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi

Eray Özgün [1] , Gülben Sayılan Özgün [2]


Aim: The aim of this study is to investigate the effect of aspirin on paraoxonase-1, paraoxonase-2 and paraoxonase-3 protein levels and arylesterase activity in human-derived hepatoma cells.

Materials and Methods: HepG2 cells were divided into four groups: control, 0.25 mM aspirin, 0.5 mM aspirin and 1 mM aspirin and cells were incubated with 0, 0.25, 0.5 and 1 mM aspirin, respectively. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Paraoxonase-1, paraoxonase-2 and paraoxonase-3 protein levels were measured by western blotting. Arylesterase activity was measured spectrophotometrically by using phenylacetate as substrate.

Results: 0.5 mM and 1 mM aspirin caused a significant decrease on cell viability. Aspirin concentrations did not significantly change paraoxonase-1 and paraoxonase-2 protein levels. 0.5 mM and 1 mM aspirin significantly increased paraoxonase-3 protein levels. 0.25 mM, 0.5 mM and 1 mM aspirin significantly increased arylesterase activity.

Conclusion: Our study showed that aspirin does not change paraoxonase-1 and paraoxonase-2 protein levels but increases paraoxonase-3 protein levels and arylesterase activity in human-derived hepatoma cells

Amaç: Bu çalışmanın amacı, aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 protein düzeylerine ve arilesteraz aktivitesine etkisini araştırmaktır.

Gereç ve Yöntem: HepG2 hücreleri kontrol, 0.25 mM aspirin, 0.5 mM aspirin ve 1 mM aspirin olmak üzere 4 gruba ayrıldı ve hücreler 48 saat boyunca sırasıyla 0, 0.25, 0.5 ve 1 mM aspirin ile inkübe edildi. Hücre canlılığı 3-(4,5-Dimetil-2-tiazolil)-2,5-difenil-2H-tetrazolium bromür testi ile ölçüldü. Paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 protein düzeyleri western blot yöntemiyle ölçüldü. Arilesteraz aktivitesi substrat olarak fenilasetat kullanılarak spektrofotometrik olarak ölçüldü.

Bulgular: 0.5 mM ve 1 mM aspirin hücre canlılığında anlamlı azalmaya yol açtı. Aspirin konsantrasyonları paraoksonaz-1 ve paraoksonaz-2 protein düzeylerini anlamlı olarak değiştirmedi. 0.5 ve 1 mM aspirin paraoksonaz-3 protein düzeylerini anlamlı olarak arttırdı. 0.25 mM, 0.5 mM ve 1 mM aspirin, arilesteraz aktivitesini anlamlı olarak arttırdı.

Sonuç: Çalışmamız aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz-1 ve paraoksonaz-2 protein düzeylerini değiştirmediğini, paraoksonaz-3 protein düzeylerini ve arilesteraz aktivitesini arttırdığını gösterdi.

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Primary Language tr
Subjects Health Care Sciences and Services
Journal Section Research Articles
Authors

Orcid: 0000-0002-6744-1519
Author: Eray Özgün (Primary Author)
Institution: Trakya Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Edirne
Country: Turkey


Orcid: 0000-0001-6990-3484
Author: Gülben Sayılan Özgün
Institution: Trakya Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Edirne
Country: Turkey


Dates

Publication Date : September 20, 2019

Bibtex @research article { etd610836, journal = {Ege Tıp Dergisi}, issn = {1016-9113}, eissn = {2147-6500}, address = {}, publisher = {Ege University}, year = {2019}, volume = {58}, pages = {295 - 302}, doi = {10.19161/etd.610836}, title = {Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi}, key = {cite}, author = {Özgün, Eray and Sayılan Özgün, Gülben} }
APA Özgün, E , Sayılan Özgün, G . (2019). Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi. Ege Tıp Dergisi , 58 (3) , 295-302 . DOI: 10.19161/etd.610836
MLA Özgün, E , Sayılan Özgün, G . "Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi". Ege Tıp Dergisi 58 (2019 ): 295-302 <http://egetipdergisi.com.tr/en/issue/47742/610836>
Chicago Özgün, E , Sayılan Özgün, G . "Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi". Ege Tıp Dergisi 58 (2019 ): 295-302
RIS TY - JOUR T1 - Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi AU - Eray Özgün , Gülben Sayılan Özgün Y1 - 2019 PY - 2019 N1 - doi: 10.19161/etd.610836 DO - 10.19161/etd.610836 T2 - Ege Tıp Dergisi JF - Journal JO - JOR SP - 295 EP - 302 VL - 58 IS - 3 SN - 1016-9113-2147-6500 M3 - doi: 10.19161/etd.610836 UR - https://doi.org/10.19161/etd.610836 Y2 - 2018 ER -
EndNote %0 Ege Tıp Dergisi Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi %A Eray Özgün , Gülben Sayılan Özgün %T Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi %D 2019 %J Ege Tıp Dergisi %P 1016-9113-2147-6500 %V 58 %N 3 %R doi: 10.19161/etd.610836 %U 10.19161/etd.610836
ISNAD Özgün, Eray , Sayılan Özgün, Gülben . "Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi". Ege Tıp Dergisi 58 / 3 (September 2019): 295-302 . https://doi.org/10.19161/etd.610836
AMA Özgün E , Sayılan Özgün G . Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi. Ege Tıp Dergisi. 2019; 58(3): 295-302.
Vancouver Özgün E , Sayılan Özgün G . Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi. Ege Tıp Dergisi. 2019; 58(3): 302-295.