Year 2020, Volume 59 , Issue 1, Pages 39 - 46 2020-03-13

Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation
Maternal uterin arter ligasyon modeli uygulanan fetal sıçanlarda kondrogenez ve osteogenezin Wnt/β-Catenin, S100 immünoekspresyon ve histomorfometri ile değerlendirilmesi

Serap USLU [1] , Gülperi ÖKTEM [2] , Fatih OLTULU [3] , Kenan DEMİR [4] , Arzu İRBAN [5] , Gülçin BAŞDEMİR [6] , Ümit İNCE [7] , Ayşegül UYSAL [8]


Aim: The aim of this study is to investigate the effects of intrauterine growth retardation depending on maternal uterine artery ligation, Wnt/β-catenin and S100 expression immunohistochemistry and histomorphometrically on chondrogenesis and osteogenesis of fetal rats.

Materials and Methods: Maternal rats were randomly divided into 3 groups (n=5). No surgery or anesthesia were applied in control group. Bilaterally the maternal uterine arteries were ligated on gestational day 18 in experimental group. Although all surgical procedures were performed in sham group, the uterine artery ligation were not made. Fetuses were taken on gestational day 20, thicknesses of growth plate and zones, trabecular number and thickness and cortical thickness were evaluated with the histomorphometrically in samples from left proximal tibia. The expressions of β-catenin and S100 immunohistochemically were evaluated in the growth plate.

Results: Thicknesses of growth plate (p<0.01), proliferation zone (p<0.05) and degeneration zone (p<0.01) were measured significantly thinner in experimental group than the others and thicknesses of hypertrophic zones were lesser than the control and sham group, but the results were not statistically significant (p>0.05). Also trabecular numbers were lower (p<0.01) and trabecular thickness were also thinner (p<0.05) in experimental group. Expression of β-catenin was declined and S100 expression was increased in experimental group.

Conclusion: We conclude that maternal uterine artery ligation, leads to shortness of growth plate and degenerated bone architecture because of Wnt/β-catenin signaling pathway.

Amaç: Bu çalışmanın amacı, sıçanlarda maternal uterin arter ligasyonuna bağlı olarak gelişen intrauterine büyüme geriliğinde kondrogenez ve osteogenezin Wnt/β-catenin ve S100 ekspresyonlarının immünohistokimyasal ve histomorfometrik olarak değerlendirilmesi.

Gereç ve Yöntem: Gebe sıçanlar 3 gruba ayrıldı (n=5). Kontrol grubuna cerrahi veya anestezi uygulanmadı. Deney grubunda, 18. gebelik gününde bilateral maternal uterin arterlere ligasyon yapıldı. Sham grubuna uterin arter ligasyonu dışındaki tüm cerrahi işlem uygulandı. Fetuslar gebeliğin 20. gününde alındı, sol proksimal tibiadan büyüme plağı ve zonların kalınlıkları, trabeküler sayı ve kalınlıkları, kortikal kalınlıklar histomorfometrik olarak değerlendirildi. Büyüme plağında β-catenin ve S100 ekspresyonları immunohistokimyasal olarak değerlendirildi.

Bulgular: Deney grubunda büyüme plağı (p<0.01), proliferasyon zonu (p<0.05) ve dejenerasyon zonun kalınlıkları (p<0.01) belirgin olarak daha ince bulundu. Hipertrofik zonların kalınlığı daha az olarak ölçüldü (p>0.05). Aynı zamanda daha düşük sayıda trabekülar yapı (p<0.01) ve daha ince trabeküler kalınlık (p<0.05) bulundu. Deney grubunda β-catenin ekspresyonu daha düşük iken S100 ekspresyonunda artış saptandı.

Sonuç: Maternal uterin arter ligasyonuna bağlı fetal sıçan intrauterine büyüme geriliği modelinde, Wnt/β-catenin sinyal yolağındaki azalmaya bağlı olarak dejenere kemik yapısı ve büyüme plağında kısalmaya yol açmaktadır.

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Primary Language en
Subjects Health Care Sciences and Services
Journal Section Research Articles
Authors

Orcid: 0000-0002-6613-527X
Author: Serap USLU (Primary Author)
Institution: Istanbul Medeniyet University, Faculty of Medicine, Department of Histology and Embryology, Istanbul
Country: Turkey


Orcid: 0000-0001-6227-9519
Author: Gülperi ÖKTEM
Institution: Ege University, Faculty of Medicine, Department of Histology and Embryology, Izmir
Country: Turkey


Orcid: 0000-0001-6475-642X
Author: Fatih OLTULU
Institution: Ege University, Faculty of Medicine, Department of Histology and Embryology, Izmir
Country: Turkey


Orcid: 0000-0003-2864-6041
Author: Kenan DEMİR
Institution: Ege University, Faculty of Medicine, Department of Histology and Embryology, Izmir
Country: Turkey


Orcid: 0000-0002-4904-0658
Author: Arzu İRBAN
Institution: University of Health Sciences, Faculty of Medicine, Department of Anesthesiology and Reanimation, Istanbul
Country: Turkey


Orcid: 0000-0002-2104-8957
Author: Gülçin BAŞDEMİR
Institution: Okan University, Faculty of Medicine, Department of Pathology, Istanbul
Country: Turkey


Orcid: 0000-0002-6113-661X
Author: Ümit İNCE
Institution: Acıbadem University, School of Medicine, Department of Pathology, Istanbul
Country: Turkey


Orcid: 0000-0002-9919-2254
Author: Ayşegül UYSAL
Institution: University of Health Sciences, Faculty of Medicine, Department of Anesthesiology and Reanimation, Istanbul
Country: Turkey


Dates

Publication Date : March 13, 2020

Bibtex @research article { etd697866, journal = {Ege Tıp Dergisi}, issn = {1016-9113}, eissn = {2147-6500}, address = {}, publisher = {Ege University}, year = {2020}, volume = {59}, pages = {39 - 46}, doi = {10.19161/etd.697866}, title = {Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation}, key = {cite}, author = {USLU, Serap and ÖKTEM, Gülperi and OLTULU, Fatih and DEMİR, Kenan and İRBAN, Arzu and BAŞDEMİR, Gülçin and İNCE, Ümit and UYSAL, Ayşegül} }
APA USLU, S , ÖKTEM, G , OLTULU, F , DEMİR, K , İRBAN, A , BAŞDEMİR, G , İNCE, Ü , UYSAL, A . (2020). Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation. Ege Tıp Dergisi , 59 (1) , 39-46 . DOI: 10.19161/etd.697866
MLA USLU, S , ÖKTEM, G , OLTULU, F , DEMİR, K , İRBAN, A , BAŞDEMİR, G , İNCE, Ü , UYSAL, A . "Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation". Ege Tıp Dergisi 59 (2020 ): 39-46 <http://egetipdergisi.com.tr/en/issue/52885/697866>
Chicago USLU, S , ÖKTEM, G , OLTULU, F , DEMİR, K , İRBAN, A , BAŞDEMİR, G , İNCE, Ü , UYSAL, A . "Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation". Ege Tıp Dergisi 59 (2020 ): 39-46
RIS TY - JOUR T1 - Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation AU - Serap USLU , Gülperi ÖKTEM , Fatih OLTULU , Kenan DEMİR , Arzu İRBAN , Gülçin BAŞDEMİR , Ümit İNCE , Ayşegül UYSAL Y1 - 2020 PY - 2020 N1 - doi: 10.19161/etd.697866 DO - 10.19161/etd.697866 T2 - Ege Tıp Dergisi JF - Journal JO - JOR SP - 39 EP - 46 VL - 59 IS - 1 SN - 1016-9113-2147-6500 M3 - doi: 10.19161/etd.697866 UR - https://doi.org/10.19161/etd.697866 Y2 - 2019 ER -
EndNote %0 Ege Tıp Dergisi Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation %A Serap USLU , Gülperi ÖKTEM , Fatih OLTULU , Kenan DEMİR , Arzu İRBAN , Gülçin BAŞDEMİR , Ümit İNCE , Ayşegül UYSAL %T Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation %D 2020 %J Ege Tıp Dergisi %P 1016-9113-2147-6500 %V 59 %N 1 %R doi: 10.19161/etd.697866 %U 10.19161/etd.697866
ISNAD USLU, Serap , ÖKTEM, Gülperi , OLTULU, Fatih , DEMİR, Kenan , İRBAN, Arzu , BAŞDEMİR, Gülçin , İNCE, Ümit , UYSAL, Ayşegül . "Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation". Ege Tıp Dergisi 59 / 1 (March 2020): 39-46 . https://doi.org/10.19161/etd.697866
AMA USLU S , ÖKTEM G , OLTULU F , DEMİR K , İRBAN A , BAŞDEMİR G , İNCE Ü , UYSAL A . Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation. ETD. 2020; 59(1): 39-46.
Vancouver USLU S , ÖKTEM G , OLTULU F , DEMİR K , İRBAN A , BAŞDEMİR G , İNCE Ü , UYSAL A . Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation. Ege Tıp Dergisi. 2020; 59(1): 46-39.