Research Article
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Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated Dental Implants

Year 2019, Volume: 9 Issue: 1, 11 - 15, 30.04.2019
https://doi.org/10.26650/experimed.2019.19002

Abstract

DOI:
10.26650/experimed.2019.19002


Objective: In our study, we aimed to investigate the relationship between the Interleukin-1β (IL-1β) +3953 gene polymorphism, the levels of this interleukin and smoking in osteointegrated dental implant losses.

Materials and Methods: 56 patients with
implants and 238 healthy control subjects were included in this study. PCR and
RFLP methods was used to determine the IL-1
β +3953 gene polymorphisms. Serum IL-1β levels were measured using ELISA.

Results: The IL-1β  +3953   CC, TT and CT genotype frequencies for the
controls and study cases were 61.2%, 
10.1% ,43.6%, and 48.2%, 8.9% and 42.9%, respectively. IL-1
β  +3953 T allele carriers
increased more in the patient group (51.8%) compared  to the control group (38.8%). These cases
also  had an increased plaque index,
bleeding index, pocket deepness and serum IL-1
β levels, but none of them were statistically significant
(p>0.05). Implant losses were more frequent in C allele carriers than in T
allele carriers, but this was not statistically significant (p>0.05) Three
of the five patients that experienced implant loss and  smoked, lost the implant earlier and had a
higher average plaque and bleeding index.

Conclusion: Based on our results, we can suggest
that being an IL-1
β positive
genotype and a smoker can have an effect on implant losses. The study group
should be enlarged to comment with statistically significant results.







Cite this article as: Dizen F, Görmüş U,
Ergen A, Ünür M, İplik ES, Çakmakoğlu B, et al. Investigation of the IL-1
β Genotype and its Levels on Patients Treated with Osteointegrated
Dental Implants. Experimed 2019; 9(1): 11-5.

References

  • 1. Mombelli A, Van Oosten MA, Schurch E, et al. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol Immunol 1987; 2: 145-51. 2. Behneke A, Behneke N, d’Hoedt B. A 5-year longitudinal study of the clinical effectiveness of ITI solid-screw implants in the treatment of mandibular edentulism. Int J Oral Maxillofac Implants 2002; 7: 799-810. 3. Ferrigno N, Laureti M, Fanali S, et al. A long-term follow-up study of non-submerged ITI implants in the treatment of totally edentulous jaws. Part I: Ten-year life table analysis of a prospective multicenter study with 1286 implants. Clin Oral Implants Res 2002; 13: 260-73. 4. Lemmerman KJ, Lemmerman NE. Osseointegrated dental implants in private practice: a long-term case series study. J Periodontol 2005; 76: 310-9. 5. Brägger U. Use of radiographs in evaluating success, stability and failure in implant dentistry. Periodontol 2000 1998; 17: 77-88. 6. Lindhe J, Berglundh T, Ericsson I, et al. Experimental breakdown of peri-implant and periodontal tissues. A study in the beagle dog. Clin Oral Implants Res 1992; 3: 9-16. 7. Esposito M, Hirsch JM, Lekholm U, et al. Biological factors contributing to failures of osseointegrated oral implants. (II). Etiopathogenesis. Eur J Oral Sci 1998; 106: 721-64. 8. Santamaria P, Gehrz RC, Bryan MK, et al. Involvement of class II MHC molecules in the LPS-induction of IL-1/TNF secretions by human monocytes. Quantitative differences at the polymorphic level. J Immunol 1989; 143: 913-22. 9. De Boever AL, De Boever JA. Early colonization of non-submerged dental implants in patients with a history of advanced aggressive periodontitis. Clin Oral Implants Res 2006; 17: 8-17. 10. Greenstein G, Hart TC. Clinical utility of a genetic susceptibility test for severe chronic periodontitis: a critical evaluation. J Am Dent Assoc 2002; 133: 452-9. 11. McGuire MK, Nunn ME. Prognosis versus actual outcome. IV. The effectiveness of clinical parameters and IL-1 genotype in accurately predicting prognoses and tooth survival. J Periodont 1997; 70: 49-56. 12. Pociot F, Molvig J, Wogensen L, et al. A TaqI polymorphism in the human interleukin-1b (IL-1b) gene correlates with IL-1b secretion in vitro. Eur J Clin Invest 1992; 22: 396-402. 13. Spiekermann H, Jansen VK, Richter EJ. A 10-year follow-up study of IMZ and TPS implants in the edentulous mandible using bar-retained overdentures. Int J Oral Maxillofac Implants 1995; 10: 231-43. 14. Branemark PI, Adell R, Albrektsson T, et al. Osseointegrated titanium fixtures in the treatment of edentulousness. Biomaterials 1983; 4: 25-8. 15. Laine ML, Leonhardt A, Roos-Jansaker A-M, et al. IL-1RN gene polymorphism is associated with peri-implantitis. Clin. Oral Impl Res 2006; 17: 380-5. 16. Kao RT, Curtis DA, Richards DW, et al. Increased interleukin-1 beta in the crevicular fluid of diseased implants. Int J Oral Maxillofac Implants 1995; 10: 696-701. 17. Panagakos FS, Aboyoussef H, Dondero R, et al. Detection and measurement of inflammatory cytokines in implant crevicular fluid: a pilot study. Int J Oral Maxillofac Implants 1996; 11: 794-9. 18. Dinarello CA. The biological properties of interleukin-1. Eur Cytokine Network 1994;5:517-531. 19. Gemmell E, Marshall RI and Seymour GJ: Cytokines and prostaglandins in immune homeostasis and tissue destruction in periodontal disease. Periodontology 2000 1997; 14: 112-43. 20. Kornman KS, Crane A, Wang HY, et al. The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol 1997; 24: 72-7. 21. Edwards RK, Ferguson RJ, Duff P. The Interleukin-1b +3953 Single Nucleotide Polymorphism: Cervical Protein Concentration and Preterm Delivery Risk American. J Reprod Immunol 2006; 55: 259-64. 22. Pietruski JK, Pietruska MD, Stokowska W, et al. Serum levels of interleukin-1 (IL-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) in patients treated with dental implants. Rocz Akad Med Bialymst 2001; 46: 28-37. 23. Sakellari D, Koukoudetsos S, Arsenakis M, et al. Prevalence of IL-1A and IL-1B polymorphisms in a Greek population. J Clin Periodontol 2003; 30: 35-41. 24. Rogers MA, Figliomeni L, Baluchova K, et al. Do interleukin-1 polymorphisms predict the development of periodontitis or the success of dental implants? J Periodontal Res 2002; 37: 37-41. 25. Feloutzis A, Lang NP, Tonetti MS, et al. IL-1 gene polymorphism and smoking as risk factors for peri-implant bone loss in a well-maintained population. Clin Oral Implants Res 2003; 14: 10-17. 26. McGuire MK, Nunn ME. Prognosis versus actual outcome. IV. The effectiveness of clinical parameters and IL-1 genotype in accurately predicting prognoses and tooth survival. J Periodontol 1999; 70: 49-56. 27. Kornman KS: Interleukin 1 genetics, inflammatory mechanisms, and nutrigenetic opportunities to modulate diseases of aging. Am J Clin Nutr 2006; 83: 475S-83S. 28. Tonetti MS. Risk factors for osseodisintegration. Periodontol 2000 1998; 17: 55-62. 29. Lindquist LW, Carlsson GE, Jemt T. Association between marginal bone loss around osseointegrated mandibular implants and smoking habits: a 10-year follow-up study. J Dent Res 1997; 76: 1667-74.

Osteointegre Dental İmplant Tedavisi Gören Hastalarda IL-1 β Genotipi ve Seviyelerinin İncelenmesi

Year 2019, Volume: 9 Issue: 1, 11 - 15, 30.04.2019
https://doi.org/10.26650/experimed.2019.19002

Abstract

DOI:
10.26650/experimed.2019.19002


Amaç: Çalışmamızda osteointegre dental
implant kayıplarında interlökin-1
β (IL-1β) +3953 gen
polimorfizminin serum interlökin düzeyleri ve sigara içimiyle ilişkisini araştırmayı
amaçladık.

Yöntemler: İmplant yapılan 56 olgu ve 238
sağlıklı kontrol öalışmaya dahil edilmiştir. IL-1
β +3953 polimorfizmi, polimeraz zincirleme reaksiyonu ve restriksiyon
fragmanı uzunluk polimorfizmi yöntemleri kullanılarak tayin edilmiştir. Serum örneklerinde
IL-1
β seviyesi ELISA yöntemi ile
saptanmıştır.

Bulgular: IL-1β  +3953 polimorfizmi; CC, TT
ve CT genotip frekansları kontrol ve hasta grubu için sırasıyla 61.2%,  10.1% , 43.6%, ve 48.2%, 8.9% ve 42.9% olarak
bulunmuştur. IL-1
β +3953 T
alleli taşıyanların hastalarda (51.8%) kontrol grubuna (38.8%) göre daha yüksek
bulunmuştur ve bu kişilerde plak indeksi, kanama indeksi, cep derinliği ve
serum IL-1
β düzeylerinin
arttığı, sigara içme ve implant kaybı oranının yükseldiği fakat istatistiksel
anlamlılığa ulaşmadığı tespit edilmiştir (p>0,05). İmplant kaybında, C
alleli taşıyanlarda T alleli taşıyanlara göre daha anlamlı bulunmasına rağmen
istatistiksel olarak anlamlı olmadığı görülmüştür (p>0,05).  İmplant kaybı olan beş hastanın üçünde IL-1
β pozitif olduğu, hepsinin sigara kullandığı, implantların erken dönemde
kaybedildiği, plak ve kanama indekslerinin ortalama değerlerden yüksek olduğu
ve cep derinliklerinin normal sınırlar arasında olduğu görülmüştür.







Sonuç: Sonuç olarak implant kaybında IL-1β pozitif genotipe sahip olma ve sigara içiminin önemli bir etken
olduğu izlenimi edinilmiş ancak olgu sayısının arttırılmasıyla istatistiksel
anlamlılığa ulaşılabileceği sonucuna ulaşılmıştır.

Cite this article as: Dizen F, Görmüş U,
Ergen A, Ünür M, İplik ES, Çakmakoğlu B, et al. Investigation of the IL-1
β Genotype and its Levels on Patients Treated with Osteointegrated
Dental Implants. Experimed 2019; 9(1): 11-5.

References

  • 1. Mombelli A, Van Oosten MA, Schurch E, et al. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol Immunol 1987; 2: 145-51. 2. Behneke A, Behneke N, d’Hoedt B. A 5-year longitudinal study of the clinical effectiveness of ITI solid-screw implants in the treatment of mandibular edentulism. Int J Oral Maxillofac Implants 2002; 7: 799-810. 3. Ferrigno N, Laureti M, Fanali S, et al. A long-term follow-up study of non-submerged ITI implants in the treatment of totally edentulous jaws. Part I: Ten-year life table analysis of a prospective multicenter study with 1286 implants. Clin Oral Implants Res 2002; 13: 260-73. 4. Lemmerman KJ, Lemmerman NE. Osseointegrated dental implants in private practice: a long-term case series study. J Periodontol 2005; 76: 310-9. 5. Brägger U. Use of radiographs in evaluating success, stability and failure in implant dentistry. Periodontol 2000 1998; 17: 77-88. 6. Lindhe J, Berglundh T, Ericsson I, et al. Experimental breakdown of peri-implant and periodontal tissues. A study in the beagle dog. Clin Oral Implants Res 1992; 3: 9-16. 7. Esposito M, Hirsch JM, Lekholm U, et al. Biological factors contributing to failures of osseointegrated oral implants. (II). Etiopathogenesis. Eur J Oral Sci 1998; 106: 721-64. 8. Santamaria P, Gehrz RC, Bryan MK, et al. Involvement of class II MHC molecules in the LPS-induction of IL-1/TNF secretions by human monocytes. Quantitative differences at the polymorphic level. J Immunol 1989; 143: 913-22. 9. De Boever AL, De Boever JA. Early colonization of non-submerged dental implants in patients with a history of advanced aggressive periodontitis. Clin Oral Implants Res 2006; 17: 8-17. 10. Greenstein G, Hart TC. Clinical utility of a genetic susceptibility test for severe chronic periodontitis: a critical evaluation. J Am Dent Assoc 2002; 133: 452-9. 11. McGuire MK, Nunn ME. Prognosis versus actual outcome. IV. The effectiveness of clinical parameters and IL-1 genotype in accurately predicting prognoses and tooth survival. J Periodont 1997; 70: 49-56. 12. Pociot F, Molvig J, Wogensen L, et al. A TaqI polymorphism in the human interleukin-1b (IL-1b) gene correlates with IL-1b secretion in vitro. Eur J Clin Invest 1992; 22: 396-402. 13. Spiekermann H, Jansen VK, Richter EJ. A 10-year follow-up study of IMZ and TPS implants in the edentulous mandible using bar-retained overdentures. Int J Oral Maxillofac Implants 1995; 10: 231-43. 14. Branemark PI, Adell R, Albrektsson T, et al. Osseointegrated titanium fixtures in the treatment of edentulousness. Biomaterials 1983; 4: 25-8. 15. Laine ML, Leonhardt A, Roos-Jansaker A-M, et al. IL-1RN gene polymorphism is associated with peri-implantitis. Clin. Oral Impl Res 2006; 17: 380-5. 16. Kao RT, Curtis DA, Richards DW, et al. Increased interleukin-1 beta in the crevicular fluid of diseased implants. Int J Oral Maxillofac Implants 1995; 10: 696-701. 17. Panagakos FS, Aboyoussef H, Dondero R, et al. Detection and measurement of inflammatory cytokines in implant crevicular fluid: a pilot study. Int J Oral Maxillofac Implants 1996; 11: 794-9. 18. Dinarello CA. The biological properties of interleukin-1. Eur Cytokine Network 1994;5:517-531. 19. Gemmell E, Marshall RI and Seymour GJ: Cytokines and prostaglandins in immune homeostasis and tissue destruction in periodontal disease. Periodontology 2000 1997; 14: 112-43. 20. Kornman KS, Crane A, Wang HY, et al. The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol 1997; 24: 72-7. 21. Edwards RK, Ferguson RJ, Duff P. The Interleukin-1b +3953 Single Nucleotide Polymorphism: Cervical Protein Concentration and Preterm Delivery Risk American. J Reprod Immunol 2006; 55: 259-64. 22. Pietruski JK, Pietruska MD, Stokowska W, et al. Serum levels of interleukin-1 (IL-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) in patients treated with dental implants. Rocz Akad Med Bialymst 2001; 46: 28-37. 23. Sakellari D, Koukoudetsos S, Arsenakis M, et al. Prevalence of IL-1A and IL-1B polymorphisms in a Greek population. J Clin Periodontol 2003; 30: 35-41. 24. Rogers MA, Figliomeni L, Baluchova K, et al. Do interleukin-1 polymorphisms predict the development of periodontitis or the success of dental implants? J Periodontal Res 2002; 37: 37-41. 25. Feloutzis A, Lang NP, Tonetti MS, et al. IL-1 gene polymorphism and smoking as risk factors for peri-implant bone loss in a well-maintained population. Clin Oral Implants Res 2003; 14: 10-17. 26. McGuire MK, Nunn ME. Prognosis versus actual outcome. IV. The effectiveness of clinical parameters and IL-1 genotype in accurately predicting prognoses and tooth survival. J Periodontol 1999; 70: 49-56. 27. Kornman KS: Interleukin 1 genetics, inflammatory mechanisms, and nutrigenetic opportunities to modulate diseases of aging. Am J Clin Nutr 2006; 83: 475S-83S. 28. Tonetti MS. Risk factors for osseodisintegration. Periodontol 2000 1998; 17: 55-62. 29. Lindquist LW, Carlsson GE, Jemt T. Association between marginal bone loss around osseointegrated mandibular implants and smoking habits: a 10-year follow-up study. J Dent Res 1997; 76: 1667-74.
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Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Article
Authors

Ferhat Dizen This is me

Uzay Görmüş This is me

Arzu Ergen This is me

Meral Ünür This is me

Elif Sinem İplik This is me

Bedia Çakmakoğlu

Turgay İsbir This is me

Publication Date April 30, 2019
Submission Date March 26, 2019
Published in Issue Year 2019 Volume: 9 Issue: 1

Cite

Vancouver Dizen F, Görmüş U, Ergen A, Ünür M, İplik ES, Çakmakoğlu B, İsbir T. Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated Dental Implants. Experimed. 2019;9(1):11-5.