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The Comparative Molecular Typing of Haemophilus Influenzae Strains Isolated from the Adenoid and Tonsils in Patients Undergoing Adenotonsillectomy

Year 2023, Volume: 13 Issue: 1, 39 - 44, 11.05.2023
https://doi.org/10.26650/experimed.1221218

Abstract

Objective: Microbiota in the upper respiratory tract begins to form immediately after birth. Aerobic and anaerobic flora will form when a healthy child reaches the age of five. Tonsillitis and adenoiditis are the most common upper respiratory tract infectious diseases in childhood and adulthood. Transmission of bacterial infections from tonsils to adenoid tissue (endogenous transmission) can be prevented by usage of suitable antibiotics. At the same time, continuous exogenous infections of each or both sites require adenotonsillectomy surgery. In this study, to understand the role of endogenous transmission of Haemophilus influenzae (H. influenzae) in adenotonsillectomy, we aimed to investigate the genetic relatedness of H. influenzae strains obtained from the tonsil and adenoid of the same patient.
Materials and Methods: Twenty eight patients were included the study for displaying the growth of 56 H. influenzae strains (28 isolates per site). We investigated the genetic relatedness of H. influenzae strains obtained from the tonsils and adenoids of the patients by using the pulsed-field gel electrophoresis (PFGE) method.
Results: Twenty one isolates were isolated from the tonsils and adenoids of unrelated patients. We excluded nine (32.2%) out of 28 patients’ H. influenzae isolates from the study, that had identical strains depending on the ≥80% similarity Dice coefficient.
Conclusion: The probability of endogenous transmission between the two sites was very high, which means that some adenotonsillectomy surgery might be avoided and treated with antibiotics.

Supporting Institution

The study was supported by the BAP (Scientific Research Projects Coordination Unit of Istanbul University, Turkey).

Project Number

17434

References

  • 1. Doğu F. Tonsil ve adenoidlerin immunobiyolojisi. J Ankara Univ Fac Med 2002; 55(4) :291-6. [CrossRef] google scholar
  • 2. Gross CW, Harrison SE. Tonsils and adenoids. Pediatr Rev 2000; 21(3): 75-8. [CrossRef] google scholar
  • 3. Zalzal GH, Cotton RA. Otolaryngology. In: Cummings CW, Fredrickson MF, Harker LA, editors. Head and Neck Surgery. St. Louis: Toronto; 1986. p.1189-211. google scholar
  • 4. Bailey BJ, Johnson JT, Newlands SD. Head & neck surgeryotolaryngology: Lippincott Williams & Wilkins; 2006. google scholar
  • 5. Özerol I, Aşgın N, Kalicioğlu M. Üst solunum yolu infeksiyonlarında Moraxella catarrhalis’in önemi. J Inonu Univ Fac Med 2002; 9(1): 25-7. google scholar
  • 6. Nakipoglu Y, Gunel G, Kanliada D, Aydemir L, Gurler N, Gurler B. An investigation of the frequency of upper respiratory tract infection pathogens and their antibiotic patterns in tonsils and adenoids of adenotonsillectomy patients. Clin Lab 2016; 62: 1547-52. [CrossRef] google scholar
  • 7. Giufrè M, Cardines R, Accogli M, Pardini M, Cerquetti M. Identification of Haemophilus influenzae clones associated with invasive disease a decade after introduction of H. influenzae serotype b vaccination in Italy. Clin Vaccine Immunol 2013; 20(8): 1223-9. [CrossRef] google scholar
  • 8. Barbosa AR, Giufre M, Cerquetti M, Bajanca-Lavado MP. Polymorphism in FtsI gene and ß-lactam susceptibility in Portuguese Haemophilus influenzae strains: Clonal dissemination of ß-lactamase-positive isolates with decreased susceptibility to amoxicillin/clavulanic acid. J Antimicrob Chemoth 2011; 66(4): 788-96. [CrossRef] google scholar
  • 9. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33(9): 2233-9. [CrossRef] google scholar
  • 10. Choi DH, Park J, Choi JK, Lee KE, Lee WH, Yang J, et al. Association between the microbiomes of tonsil and saliva samples isolated from pediatric patients subjected to tonsillectomy for the treatment of tonsillar hyperplasia. Exp Mol Med 2020; 52(9): 156473. [CrossRef] google scholar
  • 11. Oliveira Branco AA, Castro Correa C, Souza Neves D, Huehara T, Theresa Weber SA. Swallowing patterns after adenotonsillectomy in children. Pediatr Investigation 2019; 3(3): 153-8. [CrossRef] google scholar
  • 12. Fuji N, Pichichero M, Kaur R. Haemophilus influenzae prevalence, proportion of capsulated strains and antibiotic susceptibility during colonization and acute otitis media in children, 2019-2020. Pediatr Infect Dis J 2021; 27; 40(9): 792-6. [CrossRef] google scholar
  • 13. Galli J, Calò L, Posteraro B, Rossi G, Sterbini FP, Paludetti G, et al. Pediatric oropharyngeal microbiome: Mapping in chronic tonsillitis and tonsillar hypertrophy. Int J Pediatr Otorhinolaryngol 2020; 139: 110478. [CrossRef] google scholar
  • 14. Jensen A, Fagö-Olsen H, S0rensen CH, Kilian M. Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis. PloS One 2013; 8(2): e56418. [CrossRef] google scholar
  • 15. Chekrouni N, Koelman DLH, Brouwer MC, van der Ende A, van de Beek D. Community-acquired Haemophilus influenzae meningitis in adults. J Infect 2021; 82(5): 145-50. [CrossRef] google scholar
  • 16. Öncül O, Özsoy FM, İnci E, Pahsa A. Rekürren akut tonsillit olgularında tonsil yüzey, çekirdek ve arka yüz mikroflorasının karşılaştırılması. Flora 2001; 6: 101-7. google scholar
  • 17. Ünal A, Küçükoğlu S, Aslan A, Işılak I, Nalça Y. Kronik rekürren tonsillit olgularında yüzeyel ve derin tonsil florası. KBB ve BBC Derg 1998; 6: 33-6. google scholar
  • 18. Hadi U, El-Hajj M, Uwaydah M, Fuleihan N, Matar GM. Characteristics of pathogens recovered from the tonsils and adenoids in a group of Lebanese children undergoing tonsillectomy and adenoidectomy. J Appl Res 2005; 5(3); 473-80. google scholar
Year 2023, Volume: 13 Issue: 1, 39 - 44, 11.05.2023
https://doi.org/10.26650/experimed.1221218

Abstract

Project Number

17434

References

  • 1. Doğu F. Tonsil ve adenoidlerin immunobiyolojisi. J Ankara Univ Fac Med 2002; 55(4) :291-6. [CrossRef] google scholar
  • 2. Gross CW, Harrison SE. Tonsils and adenoids. Pediatr Rev 2000; 21(3): 75-8. [CrossRef] google scholar
  • 3. Zalzal GH, Cotton RA. Otolaryngology. In: Cummings CW, Fredrickson MF, Harker LA, editors. Head and Neck Surgery. St. Louis: Toronto; 1986. p.1189-211. google scholar
  • 4. Bailey BJ, Johnson JT, Newlands SD. Head & neck surgeryotolaryngology: Lippincott Williams & Wilkins; 2006. google scholar
  • 5. Özerol I, Aşgın N, Kalicioğlu M. Üst solunum yolu infeksiyonlarında Moraxella catarrhalis’in önemi. J Inonu Univ Fac Med 2002; 9(1): 25-7. google scholar
  • 6. Nakipoglu Y, Gunel G, Kanliada D, Aydemir L, Gurler N, Gurler B. An investigation of the frequency of upper respiratory tract infection pathogens and their antibiotic patterns in tonsils and adenoids of adenotonsillectomy patients. Clin Lab 2016; 62: 1547-52. [CrossRef] google scholar
  • 7. Giufrè M, Cardines R, Accogli M, Pardini M, Cerquetti M. Identification of Haemophilus influenzae clones associated with invasive disease a decade after introduction of H. influenzae serotype b vaccination in Italy. Clin Vaccine Immunol 2013; 20(8): 1223-9. [CrossRef] google scholar
  • 8. Barbosa AR, Giufre M, Cerquetti M, Bajanca-Lavado MP. Polymorphism in FtsI gene and ß-lactam susceptibility in Portuguese Haemophilus influenzae strains: Clonal dissemination of ß-lactamase-positive isolates with decreased susceptibility to amoxicillin/clavulanic acid. J Antimicrob Chemoth 2011; 66(4): 788-96. [CrossRef] google scholar
  • 9. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33(9): 2233-9. [CrossRef] google scholar
  • 10. Choi DH, Park J, Choi JK, Lee KE, Lee WH, Yang J, et al. Association between the microbiomes of tonsil and saliva samples isolated from pediatric patients subjected to tonsillectomy for the treatment of tonsillar hyperplasia. Exp Mol Med 2020; 52(9): 156473. [CrossRef] google scholar
  • 11. Oliveira Branco AA, Castro Correa C, Souza Neves D, Huehara T, Theresa Weber SA. Swallowing patterns after adenotonsillectomy in children. Pediatr Investigation 2019; 3(3): 153-8. [CrossRef] google scholar
  • 12. Fuji N, Pichichero M, Kaur R. Haemophilus influenzae prevalence, proportion of capsulated strains and antibiotic susceptibility during colonization and acute otitis media in children, 2019-2020. Pediatr Infect Dis J 2021; 27; 40(9): 792-6. [CrossRef] google scholar
  • 13. Galli J, Calò L, Posteraro B, Rossi G, Sterbini FP, Paludetti G, et al. Pediatric oropharyngeal microbiome: Mapping in chronic tonsillitis and tonsillar hypertrophy. Int J Pediatr Otorhinolaryngol 2020; 139: 110478. [CrossRef] google scholar
  • 14. Jensen A, Fagö-Olsen H, S0rensen CH, Kilian M. Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis. PloS One 2013; 8(2): e56418. [CrossRef] google scholar
  • 15. Chekrouni N, Koelman DLH, Brouwer MC, van der Ende A, van de Beek D. Community-acquired Haemophilus influenzae meningitis in adults. J Infect 2021; 82(5): 145-50. [CrossRef] google scholar
  • 16. Öncül O, Özsoy FM, İnci E, Pahsa A. Rekürren akut tonsillit olgularında tonsil yüzey, çekirdek ve arka yüz mikroflorasının karşılaştırılması. Flora 2001; 6: 101-7. google scholar
  • 17. Ünal A, Küçükoğlu S, Aslan A, Işılak I, Nalça Y. Kronik rekürren tonsillit olgularında yüzeyel ve derin tonsil florası. KBB ve BBC Derg 1998; 6: 33-6. google scholar
  • 18. Hadi U, El-Hajj M, Uwaydah M, Fuleihan N, Matar GM. Characteristics of pathogens recovered from the tonsils and adenoids in a group of Lebanese children undergoing tonsillectomy and adenoidectomy. J Appl Res 2005; 5(3); 473-80. google scholar
There are 18 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Article
Authors

Gülşen Günel 0000-0003-1574-0231

Levent Aydemir 0000-0002-5836-4304

Özlem Ünaldı 0000-0002-5560-6558

Yasar Nakipoğlu 0000-0001-7979-7291

Project Number 17434
Publication Date May 11, 2023
Submission Date January 10, 2023
Published in Issue Year 2023 Volume: 13 Issue: 1

Cite

Vancouver Günel G, Aydemir L, Ünaldı Ö, Nakipoğlu Y. The Comparative Molecular Typing of Haemophilus Influenzae Strains Isolated from the Adenoid and Tonsils in Patients Undergoing Adenotonsillectomy. Experimed. 2023;13(1):39-44.