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In Vitro Anti-Helicobacter Pylori and Antimycobacterial Activity Evaluation Of Selected Plants From Turkey

Year 2020, Volume: 5 Issue: 2, 231 - 235, 30.06.2020
https://doi.org/10.35229/jaes.689341

Abstract

In this study, the extracts of Ulmus minor Mill. subsp. minor (Ulmaceae), Lathyrus pratensis L. (Leguminosae), Glaucium leiocarpum Bois. (Papaveraceae), and Echium vulgare L. (Boraginacea) were investigated for their in vitro anti-Helicobacter pylori and anti-mycobacterial activity.
The air-dried plant material was powdered and extracted with 70% ethanol by maceration. The extract was filtered and evaporated to dryness under the vacuum and then dissolved in a water-ethanol (90:10) mixture and extracted with dichloromethane and ethyl acetate, respectively. Antibacterial activity was investigated by microdilution method against Helicobacter pylori ATCC 43504, Mycobacterium smegmatis ATCC 14468 and Mycobacterium avium ATCC 25291.
The ethyl acetate extract of U. minor showed activity with MIC of 250 µg / mL against H. pylori. The other extracts showed no or weak inhibitory activity at >2000 µg/mL concentrations against the tested microorganisms. In conclusion, ethyl acetate extract of U. minor may be used for the treatment of H. pylori infections.

References

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  • Baytop T. (1984). Therapy with medicinal plants in Turkey (Past and Present), (p.189) Publication no 325, Istanbul University, Istanbul.
  • Bournine, L., Bensalem, S., Wauters, J. N., Iguer-Ouada, M., Maiza-Benabdesselam, F., Bedjou, F., ... & Frédérich, M. (2013). Identification and quantification of the main active anticancer alkaloids from the root of Glaucium flavum. International journal of molecular sciences, 14(12), 23533-23544.
  • Bulut, G., Haznedaroğlu, M. Z., Doğan, A., Koyu, H., & Tuzlacı, E. (2017). An ethnobotanical study of medicinal plants in Acipayam (Denizli-Turkey). Journal of Herbal Medicine, 10, 64-81.
  • Choi, S. Y., Lee, S., Choi, W. H., Lee, Y., Jo, Y. O., & Ha, T. Y. (2010). Isolation and anti-inflammatory activity of Bakuchiol from Ulmus davidiana var. japonica. Journal of medicinal food, 13(4), 1019-1023.
  • Cullen, J. (1965) Glaucium Mill. in: Davis P.H. (Ed.) Flora of Turkey and the East Aegean islands, Vol 1. Edinburgh University Press, Edinburgh, pp. 214–217.
  • Davis PH. (1982). Flora of Turkey and East Aegean Islands, University Press, Edinburgh.
  • Eşen, B. (2008). Aydınlar Köyü ve çevresinin (Erdemli/Mersin) etnobotanik özellikleri, (p.110), (Doctoral dissertation, Selçuk Üniversitesi Fen Bilimleri Enstitüsü).
  • Hakemi-Vala, M., Mehrara, M., Pourramezan, M., Rahimifard, J. A. N., Khoshnood, S., & Heidary, M. (2017). Comparison the Antimicrobial Effects of the Flowering Aerial Parts of Glaucium vitellinum Boiss. and Buhse and Gaillonia aucheri Jaub. and Spach. Novelty in Biomedicine, 5, 24-29.
  • Heydari, H., Saltan İşcan, G., Eryılmaz, M., Bahadır Acıkara, Ö., Yılmaz Sarıaltın, S., Tekin, M., & Çoban, T. (2019). Antimicrobial and Anti-Inflammatory Activity of Some Lathyrus L. (Fabaceae) Species Growing in Turkey. Turkish Journal of Pharmaceutical Sciences, 16(2).
  • Klemow, K. M., Clements, D. R., Threadgill, P. F., & Cavers, P. B. (2002). The biology of Canadian weeds. 116. Echium vulgare L. Canadian Journal of Plant Science, 82(1), 235-248.
  • Kuruüzüm-Uz, A., Güvenalp, Z., Ströch, K., Demirezer, L. Ö., & Zeeck, A. (2004). Phytochemical and antimicrobial investigation of Echium vulgare growing in Turkey. Biochemical Systematics and Ecology, 32(9), 833-836.
  • Lee, G. Y., Jang, D. S., Kim, J., Kim, C. S., Kim, Y. S., Kim, J. H., & Kim, J. S. (2008). Flavan-3-ols from Ulmus davidiana var. japonica with inhibitory activity on protein glycation. Planta medica, 74(15), 1800-1802.
  • Manganelli, R. U., Camangi, F., & Tomei, P. E. (2001). Curing animals with plants: traditional usage in Tuscany (Italy). Journal of Ethnopharmacology, 78(2-3), 171-191.
  • Morteza-Semnani, K., Amin, G., Shidfar, M. R., Hadizadeh, H., & Shafiee, A. (2003). Antifungal activity of the methanolic extract and alkaloids of Glaucium oxylobum. Fitoterapia, 74(5), 493-496.
  • Orhan, I., Şener, B., Choudhary, M. I., & Khalid, A. (2004). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. Journal of ethnopharmacology, 91(1), 57-60.
  • Papageorgiou, V. P., Assimopoulou, A. N., Couladouros, E. A., Hepworth, D., & Nicolaou, K. C. (1999). The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. Angewandte Chemie International Edition, 38(3), 270-301.
  • Parsons, W. T., Parsons, W. T., & Cuthbertson, E. G. (2001). Noxious weeds of Australia. CSIRO publishing.
  • Soureshjan, E. H., & Heidari, M. (2014). In vitro Variation in antibacterial activity plant extracts on Glaucium elegans and Saffron (Crocus sativus L.) Onios. Electronic Journal of Biology, 10, 64-67.
  • Tağ, Ö., Yaşa, I., Polat, E., Özgökçe, F., & Karayıldırım, T. (2011). Anti-microbial activities of leaves and stems of Ulmus minor Miller subsp. minor. Planta Medica, 77(12), PM151.
  • Whitmire, J. M., & Merrell, D. S. (2012). Successful culture techniques for Helicobacter species: general culture techniques for Helicobacter pylori. In Helicobacter Species (pp. 17-27). Humana Press, Totowa, NJ.
  • Wikler, M. A. (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. CLSI (NCCLS), 26, M7-A7.
  • Yeşilada, E., Sezik, E., Honda, G., Takaishi, Y., Takeda, Y., & Tanaka, T. (1999). Traditional medicine in Turkey IX: Folk medicine in north-west Anatolia. Journal of Ethnopharmacology, 64(3), 195-210.
  • Zheng, M. S., Yang, J. H., Li, Y., Li, X., Chang, H. W., & Son, J. K. (2010). Anti-inflammatory activity of constituents isolated from Ulmus davidiana var. japonica. Biomolecules & Therapeutics, 18(3), 321-328.
  • Zhu, X., Skoneczny, D., Weidenhamer, J. D., Mwendwa, J. M., Weston, P. A., Gurr, G. M., ... & Weston, L. A. (2016). Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson's curse (Echium plantagineum), a noxious invader. Journal of Experimental Botany, 67(12), 3777-3788.
Year 2020, Volume: 5 Issue: 2, 231 - 235, 30.06.2020
https://doi.org/10.35229/jaes.689341

Abstract

References

  • Arabi, Z., & Sardari, S. (2010). An investigation into the antifungal property of Fabaceae using bioinformatics tools. Avicenna journal of medical biotechnology, 2(2), 93.
  • Baytop T. (1984). Therapy with medicinal plants in Turkey (Past and Present), (p.189) Publication no 325, Istanbul University, Istanbul.
  • Bournine, L., Bensalem, S., Wauters, J. N., Iguer-Ouada, M., Maiza-Benabdesselam, F., Bedjou, F., ... & Frédérich, M. (2013). Identification and quantification of the main active anticancer alkaloids from the root of Glaucium flavum. International journal of molecular sciences, 14(12), 23533-23544.
  • Bulut, G., Haznedaroğlu, M. Z., Doğan, A., Koyu, H., & Tuzlacı, E. (2017). An ethnobotanical study of medicinal plants in Acipayam (Denizli-Turkey). Journal of Herbal Medicine, 10, 64-81.
  • Choi, S. Y., Lee, S., Choi, W. H., Lee, Y., Jo, Y. O., & Ha, T. Y. (2010). Isolation and anti-inflammatory activity of Bakuchiol from Ulmus davidiana var. japonica. Journal of medicinal food, 13(4), 1019-1023.
  • Cullen, J. (1965) Glaucium Mill. in: Davis P.H. (Ed.) Flora of Turkey and the East Aegean islands, Vol 1. Edinburgh University Press, Edinburgh, pp. 214–217.
  • Davis PH. (1982). Flora of Turkey and East Aegean Islands, University Press, Edinburgh.
  • Eşen, B. (2008). Aydınlar Köyü ve çevresinin (Erdemli/Mersin) etnobotanik özellikleri, (p.110), (Doctoral dissertation, Selçuk Üniversitesi Fen Bilimleri Enstitüsü).
  • Hakemi-Vala, M., Mehrara, M., Pourramezan, M., Rahimifard, J. A. N., Khoshnood, S., & Heidary, M. (2017). Comparison the Antimicrobial Effects of the Flowering Aerial Parts of Glaucium vitellinum Boiss. and Buhse and Gaillonia aucheri Jaub. and Spach. Novelty in Biomedicine, 5, 24-29.
  • Heydari, H., Saltan İşcan, G., Eryılmaz, M., Bahadır Acıkara, Ö., Yılmaz Sarıaltın, S., Tekin, M., & Çoban, T. (2019). Antimicrobial and Anti-Inflammatory Activity of Some Lathyrus L. (Fabaceae) Species Growing in Turkey. Turkish Journal of Pharmaceutical Sciences, 16(2).
  • Klemow, K. M., Clements, D. R., Threadgill, P. F., & Cavers, P. B. (2002). The biology of Canadian weeds. 116. Echium vulgare L. Canadian Journal of Plant Science, 82(1), 235-248.
  • Kuruüzüm-Uz, A., Güvenalp, Z., Ströch, K., Demirezer, L. Ö., & Zeeck, A. (2004). Phytochemical and antimicrobial investigation of Echium vulgare growing in Turkey. Biochemical Systematics and Ecology, 32(9), 833-836.
  • Lee, G. Y., Jang, D. S., Kim, J., Kim, C. S., Kim, Y. S., Kim, J. H., & Kim, J. S. (2008). Flavan-3-ols from Ulmus davidiana var. japonica with inhibitory activity on protein glycation. Planta medica, 74(15), 1800-1802.
  • Manganelli, R. U., Camangi, F., & Tomei, P. E. (2001). Curing animals with plants: traditional usage in Tuscany (Italy). Journal of Ethnopharmacology, 78(2-3), 171-191.
  • Morteza-Semnani, K., Amin, G., Shidfar, M. R., Hadizadeh, H., & Shafiee, A. (2003). Antifungal activity of the methanolic extract and alkaloids of Glaucium oxylobum. Fitoterapia, 74(5), 493-496.
  • Orhan, I., Şener, B., Choudhary, M. I., & Khalid, A. (2004). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. Journal of ethnopharmacology, 91(1), 57-60.
  • Papageorgiou, V. P., Assimopoulou, A. N., Couladouros, E. A., Hepworth, D., & Nicolaou, K. C. (1999). The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. Angewandte Chemie International Edition, 38(3), 270-301.
  • Parsons, W. T., Parsons, W. T., & Cuthbertson, E. G. (2001). Noxious weeds of Australia. CSIRO publishing.
  • Soureshjan, E. H., & Heidari, M. (2014). In vitro Variation in antibacterial activity plant extracts on Glaucium elegans and Saffron (Crocus sativus L.) Onios. Electronic Journal of Biology, 10, 64-67.
  • Tağ, Ö., Yaşa, I., Polat, E., Özgökçe, F., & Karayıldırım, T. (2011). Anti-microbial activities of leaves and stems of Ulmus minor Miller subsp. minor. Planta Medica, 77(12), PM151.
  • Whitmire, J. M., & Merrell, D. S. (2012). Successful culture techniques for Helicobacter species: general culture techniques for Helicobacter pylori. In Helicobacter Species (pp. 17-27). Humana Press, Totowa, NJ.
  • Wikler, M. A. (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. CLSI (NCCLS), 26, M7-A7.
  • Yeşilada, E., Sezik, E., Honda, G., Takaishi, Y., Takeda, Y., & Tanaka, T. (1999). Traditional medicine in Turkey IX: Folk medicine in north-west Anatolia. Journal of Ethnopharmacology, 64(3), 195-210.
  • Zheng, M. S., Yang, J. H., Li, Y., Li, X., Chang, H. W., & Son, J. K. (2010). Anti-inflammatory activity of constituents isolated from Ulmus davidiana var. japonica. Biomolecules & Therapeutics, 18(3), 321-328.
  • Zhu, X., Skoneczny, D., Weidenhamer, J. D., Mwendwa, J. M., Weston, P. A., Gurr, G. M., ... & Weston, L. A. (2016). Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson's curse (Echium plantagineum), a noxious invader. Journal of Experimental Botany, 67(12), 3777-3788.
There are 25 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Ayşe Esra Karadağ 0000-0002-3412-0807

Ayşegül Çaşkurlu This is me 0000-0001-7277-920X

Fatma Tosun 0000-0003-2533-5141

Publication Date June 30, 2020
Submission Date February 14, 2020
Acceptance Date June 5, 2020
Published in Issue Year 2020 Volume: 5 Issue: 2

Cite

APA Karadağ, A. E., Çaşkurlu, A., & Tosun, F. (2020). In Vitro Anti-Helicobacter Pylori and Antimycobacterial Activity Evaluation Of Selected Plants From Turkey. Journal of Anatolian Environmental and Animal Sciences, 5(2), 231-235. https://doi.org/10.35229/jaes.689341


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