Abstract
Aim:
Brucellosis is a zoonotic infection that is common in both animals and humans
all over the world, caused by Brucella bacteria. In this study, it was aimed to
identify species of Brucella bacterial isolates isolated in Adıyaman province
and to determine biotype distribution and profiles.
Material and Methods: Fourty patients (22 males, 18
females) that Brucella grew in their
blood cultures,
were included in the study. Patient
samples were incubated for 10 days for blood culture. Positive signaling
samples were plated on 5% sheep blood agar medium. Then, they were incubated
for 48 hours at 37 °C. For those who were identified as Gram negative
coccobacilli, using by Gram staining, serologic confirmation was performed. The
bacterial strains were identified using an antibiogram device. For the
genotyping, the Polymerase Chain Reaction method was used.
Results: Of the
40 patients included in the study, 22 were male and 18 were female. All of the
isolated bacteria were detected as B.
melitensis with fully automated culture antibiogram device. The biovar
studying failed due to an unknown inhibition in the amplification of the
nucleic acid material.
Conclusion: As in our country, B. melitensis was detected as the causative factor in the majority
of cases of brucellosis in humans, in our province too.
References
- 1. Bilgehan H: Klinik Mikrobiyoloji Ozel Bakteriyoloji ve Bakteri Enfeksiyonları. 199-204, 10. Baskı, Barıs Yayınları, Fakulteler Kitabevi, İzmir, 2000. 2. Gotuzzo E, Cellillo C. Brucella. In: Gorbach SL, Bartlett JG, Blacklow NR (eds). Infectious Diseases. 2nd Edition. W.B. Saunders Co., Philadelphia, 1992; 1513- 1521. 3. Yuce A, Alp Cavus S. Brucellosis in Turkey. A review. Klimik Journal 2006;19:87–97. 4. Lindquist D, Chu MC and Probert WS. (Ceviren Z.Cetinkaya): Francisella ve Brucella, “Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (eds) (Ceviri ed: A.Basustaoğlu): Klinik Mikrobiyoloji, 9.baskı, s.815-834, Atlas Kitapcılık, Ankara 2009. 5. Kose S, Kilic S and Ozbel Y: Identification of Brucella species isolated from proven brucellosis patients in Izmir, Turkey, J Basic Microbiol; 45(4): 323-327, 2005. 6. Cerekci A, Kilic S, Bayraktar M, Uyanik MH, Ekrem Yasar E, Esen B. Comparison of Conventional Methods and Real-Time Multiplex Polymerase Chain Reaction for Identification and Typing of Brucella Isolates of Human Origin. Mikrobiyol Bul 2011; 45(3): 392-400) 7. Hall WH: Modern chemotherapy for brucellosis in humans, Rev Infect Dis; 12(6): 1060-1099, 1990. 8. Ozsut H: Bruselloz tedavisi. Klimik Derg; 3(1): 26-29, 1990. 9. Rolain JM, Maurin M and Raoult D: Bactericidal effect of antibiotics on Bartonella and Brucella spp.: clinical implications, J Antimicrob Chemother; 46(5): 811- 814, 2000. 10. Anğ O ve Yumuk Z: Bruselloz. Cev: Madkour’s Brucellosis. Ed: M.M.Madkour, Nobel Tıp Kitabevleri, İstanbul, 2008. 11. Smith LD and Ficht TA: Pathogenesis of Brucella. Crit Rev Microbiol; 17: 209-230, 1990. 12. Morata P, Queipo-Ortuno MI, Reguera JM, Miralles F, Lopez-Gonzalez JJ and Colmenero JD: Diagnostic yield of a PCR assay in focal complications of brucellosis, J Clin Microbiol. 2001; 39(10): 3743-3746 13. Bricker BJ, Ewalt DR and Halling SM: Brucella 'HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs), BMC Microbiol.; 11(3): 15, 2003. 14. Coppola N: New diagnostic frontiers in brucellosis, Infez Med; 9(3): 130- 136, 2001. 15. Eşel D, Sümerkan B, Ayangil D, Telli M. Brucella melitensıs suşlarının antibiyotik duyarlılıklarının belirlenmesinde agar dilüsyon ve e-test yöntemlerinin karşılaştırılması. ANKEM Derg 2004;18(4):196-199. 16. Navarro E, Casao MA, Solera J. Diagnosis of human brucellosis using PCR. Expert Rev Mol Diagn 2004; 4(1): 115-23. 17. Cengiz AT ve Dolapcı Gİ: Brucella’ların ozellikleri ve brusellozda tanı yontemleri, Ankara U Tıp Fak Mec; 50(1): 41-46, 1997. 18. Aktas O: Brusellozda Mikrobiyolojik Tanı, ANKEM Derg; 17(3): 336-339, 2003. 19. Young EJ. Brucella species. In: Mandel Gl, Bennet JE, Dolin R. Principles and Practice of Infectious Diseases. Churchill Livingstone. Philadelphia, 2000; 2386-2393. 20. Sozen TH. Bruselloz. In “İnfeksiyon Hastalıkları ve Mikrobiyolojisi” Ed. Willke Topcu A, Soyletir G, Doğanay M. 486-91, Nobel Tıp Kitabevleri, İstanbul, 1996. 21. Kılıc D, Kurt H, Sozen TH ve Kandilci S: Kan Kulturlerinden izole edilen Brucella cinsi bakterilerin antibiyotiklere duyarlılıkları ve klinik yönünden değerlendirilmesi, İnfeksiyon Derg; 8(1-2): 59-62, 1994. 22. Cekovska Z, Petrovska M, Jankoska G, Panovski N, Kaftandzieva A. Isolation, identification and antimicrobial susceptibility of brucella blood culture isolates. Prilozi. 2010;31(1):117-32. 23. Zahidi JM, Yong TB, Hashim R, Noor AM, Hamzah SH, Ahmad N. Identification of Brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis. Diagnostic Microbiology and Infectious Disease 81 (2015) 227–233 24. Mustafa AS, Habibi N, Osman A, Shaheed F, and Khan MW. Species identification and molecular typing of human Brucella isolates from Kuwait. PLoS One. 2017; 12(8): e0182111. 25. Simsek H, Erdenlig S, Oral B, Tülek N. İnsan Kaynaklı Brucella izolatlarının Tip-Biyotip Tayini ve Epidemiyolojik Olarak irdelenmesi. Klimik Derg 2004; 17(2):103-106 26. Mugizi DR, Muradrasoli S, Boqvist S, Erume J, Nasinyama GW, Waiswa C, Mboowa G, Klint M, and Magnusson U. Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda. Biomed Res Int. 2015; 2015: 720413.
Abstract
Amaç: Brucellosis, Brucella cinsi bakterilerin neden olduğu tüm
dünyada hem hayvanlarda hem de insanlarda yaygın olarak görülen zoonotik bir
enfeksiyondur. Bu çalışmada, Adıyaman ilinde izole edilen Brucella cinsi
bakterilerin türlerinin identifikasyonu yapılarak, biyotip dağılımı ve
profillerinin belirlenmesi amaçlandı.
Bulgular: Çalışmaya
alınan 40 hastanın 22’si erkek, 18’i kadındı. İzole edilen bakterilerin tümü,
tam otomatize kültür antibiyogram cihazı ile
Brucella melitensis (B. melitensis) olarak saptandı. Biyovar tiplendirme
çalışması, nedeni anlaşılamayan bir inhibisyondan dolayı, nükleik materyal
amplifikasyonu başarısızlıkla sonuçlandı.
Sonuçlar: Ülkemizde olduğu gibi ilimizde de insanlarda Bruselloz
olgularının büyük çoğunluğunda etken olarak Brucella
melitensis saptandı.
Keywords
References
- 1. Bilgehan H: Klinik Mikrobiyoloji Ozel Bakteriyoloji ve Bakteri Enfeksiyonları. 199-204, 10. Baskı, Barıs Yayınları, Fakulteler Kitabevi, İzmir, 2000. 2. Gotuzzo E, Cellillo C. Brucella. In: Gorbach SL, Bartlett JG, Blacklow NR (eds). Infectious Diseases. 2nd Edition. W.B. Saunders Co., Philadelphia, 1992; 1513- 1521. 3. Yuce A, Alp Cavus S. Brucellosis in Turkey. A review. Klimik Journal 2006;19:87–97. 4. Lindquist D, Chu MC and Probert WS. (Ceviren Z.Cetinkaya): Francisella ve Brucella, “Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (eds) (Ceviri ed: A.Basustaoğlu): Klinik Mikrobiyoloji, 9.baskı, s.815-834, Atlas Kitapcılık, Ankara 2009. 5. Kose S, Kilic S and Ozbel Y: Identification of Brucella species isolated from proven brucellosis patients in Izmir, Turkey, J Basic Microbiol; 45(4): 323-327, 2005. 6. Cerekci A, Kilic S, Bayraktar M, Uyanik MH, Ekrem Yasar E, Esen B. Comparison of Conventional Methods and Real-Time Multiplex Polymerase Chain Reaction for Identification and Typing of Brucella Isolates of Human Origin. Mikrobiyol Bul 2011; 45(3): 392-400) 7. Hall WH: Modern chemotherapy for brucellosis in humans, Rev Infect Dis; 12(6): 1060-1099, 1990. 8. Ozsut H: Bruselloz tedavisi. Klimik Derg; 3(1): 26-29, 1990. 9. Rolain JM, Maurin M and Raoult D: Bactericidal effect of antibiotics on Bartonella and Brucella spp.: clinical implications, J Antimicrob Chemother; 46(5): 811- 814, 2000. 10. Anğ O ve Yumuk Z: Bruselloz. Cev: Madkour’s Brucellosis. Ed: M.M.Madkour, Nobel Tıp Kitabevleri, İstanbul, 2008. 11. Smith LD and Ficht TA: Pathogenesis of Brucella. Crit Rev Microbiol; 17: 209-230, 1990. 12. Morata P, Queipo-Ortuno MI, Reguera JM, Miralles F, Lopez-Gonzalez JJ and Colmenero JD: Diagnostic yield of a PCR assay in focal complications of brucellosis, J Clin Microbiol. 2001; 39(10): 3743-3746 13. Bricker BJ, Ewalt DR and Halling SM: Brucella 'HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs), BMC Microbiol.; 11(3): 15, 2003. 14. Coppola N: New diagnostic frontiers in brucellosis, Infez Med; 9(3): 130- 136, 2001. 15. Eşel D, Sümerkan B, Ayangil D, Telli M. Brucella melitensıs suşlarının antibiyotik duyarlılıklarının belirlenmesinde agar dilüsyon ve e-test yöntemlerinin karşılaştırılması. ANKEM Derg 2004;18(4):196-199. 16. Navarro E, Casao MA, Solera J. Diagnosis of human brucellosis using PCR. Expert Rev Mol Diagn 2004; 4(1): 115-23. 17. Cengiz AT ve Dolapcı Gİ: Brucella’ların ozellikleri ve brusellozda tanı yontemleri, Ankara U Tıp Fak Mec; 50(1): 41-46, 1997. 18. Aktas O: Brusellozda Mikrobiyolojik Tanı, ANKEM Derg; 17(3): 336-339, 2003. 19. Young EJ. Brucella species. In: Mandel Gl, Bennet JE, Dolin R. Principles and Practice of Infectious Diseases. Churchill Livingstone. Philadelphia, 2000; 2386-2393. 20. Sozen TH. Bruselloz. In “İnfeksiyon Hastalıkları ve Mikrobiyolojisi” Ed. Willke Topcu A, Soyletir G, Doğanay M. 486-91, Nobel Tıp Kitabevleri, İstanbul, 1996. 21. Kılıc D, Kurt H, Sozen TH ve Kandilci S: Kan Kulturlerinden izole edilen Brucella cinsi bakterilerin antibiyotiklere duyarlılıkları ve klinik yönünden değerlendirilmesi, İnfeksiyon Derg; 8(1-2): 59-62, 1994. 22. Cekovska Z, Petrovska M, Jankoska G, Panovski N, Kaftandzieva A. Isolation, identification and antimicrobial susceptibility of brucella blood culture isolates. Prilozi. 2010;31(1):117-32. 23. Zahidi JM, Yong TB, Hashim R, Noor AM, Hamzah SH, Ahmad N. Identification of Brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis. Diagnostic Microbiology and Infectious Disease 81 (2015) 227–233 24. Mustafa AS, Habibi N, Osman A, Shaheed F, and Khan MW. Species identification and molecular typing of human Brucella isolates from Kuwait. PLoS One. 2017; 12(8): e0182111. 25. Simsek H, Erdenlig S, Oral B, Tülek N. İnsan Kaynaklı Brucella izolatlarının Tip-Biyotip Tayini ve Epidemiyolojik Olarak irdelenmesi. Klimik Derg 2004; 17(2):103-106 26. Mugizi DR, Muradrasoli S, Boqvist S, Erume J, Nasinyama GW, Waiswa C, Mboowa G, Klint M, and Magnusson U. Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda. Biomed Res Int. 2015; 2015: 720413.
Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Original article |
| Authors | |
| Publication Date | December 1, 2017 |
| Published in Issue | Year 2017 Volume: 9 Issue: 4 |
e-ISSN: 2548-0251
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