[ Ana Sayfa | Amaç ve Kapsam | Yayın Kurulu | İçindekiler | Arşiv | Yayın Arama | Yazım Kuralları | İletişim ]
Ege Journal of Medicine
2014, Cilt 53, Sayı 4, Sayfa(lar) 184-188
[ Türkçe Özet ] [ Tam Metin ] [ PDF ] [ Benzer Makaleler ]
Identification of t(15;17) PML-RARA translocation in acute promyelocytic leukemia prediagnosed children and adult cases by real time qRT-PCR with 5 year follow up results
Tezcanlı Kaymaz B1, Bozok Çetintaş V1, Tetik Vardarlı A1, Yılmaz Süslüer S1, Aygüneş D1, Dalmızrak A1, Aktan Ç1, Küçükaslan A Ş1, Balcı T1, Kayabaşı Ç1, Özmen Yelken B1, Selvi Günel N1, Biray Avcı Ç1, Kosova B1, Eroğlu Z1, Aksoylar S2, Çetingül N2, Balkan C3, Yılmaz D3, Aydınok Y3, Kavaklı K3, Töbü M4, Tombuloğlu M4, Şahin F4, Büyükkeçeci F4, Saydam G4, Gündüz C1
1Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İzmir, Türkiye
2Ege Üniversitesi Tıp Fakültesi, Çocuk Onkoloji Bilim Dalı, İzmir, Türkiye
3Ege Üniversitesi Tıp Fakültesi, Çocuk Hematoloji Bilim Dalı, İzmir, Türkiye
4Ege Üniversitesi Tıp Fakültesi, Hematoloji Bilim Dalı, İzmir, Türkiye
Keywords: Acute promyelocytic leukemia, minimal residual disease, PML/RARA, qRT-PCR, t(15;17)

Aim: Acute promyelocytic leukemia (APL) is a well-defined subtype of acute myeloid leukemia (AML) specifically characterized by the t(15;17)(q22;q12) translocation. t(15;17) results in the fusion of the genes, promyelocytic leukemia (PML) on chromosome 15 and retinoic acid receptor alpha (RARA) located on 17th chromosome. Translocation is detected by conventional cytogenetic, fluorescence in situ hybridization analyses (FISH) and often a real time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) method. In this study, quantification of t(15; 17) translocation via real time qRT-PCR was aimed in blood or bone marrow materials belonging to APL pre-diagnosed cases that appealed our department.

Materials and Methods: Seventy nine children (7.28 ± 5.20 years; 45 M, 34 F) and 359 adults (47.71 ± 15.57 years; 193 M, 166 F) were included in the study between the years 2009-2013. Following total RNA isolation from blood or bone marrow materials of the cases, cDNA synthesis was carried out. Then, t(15; 17) translocation was studied by qRT-PCR and quantitated.

Results: Two cases from children (3%), and in total 6 tests (5%) were detected positive for t(15;17). The average t(15;17) quantification value was 0.0002 ± 0.0003. Twenty six cases of the adults (7%), in total 30 tests (8%) were determined as t(15;17) positive. Average t(15;17) quantification value of these cases was 0.067 ± 0.144.

Conclusion: The superiority of qRT-PCR compared to conventional cytogenetic studies can be found in the fact that all working steps can be monitored simultaneously during the test and the resulting amplicons can be quantitated. The clinical significance of t(15;17) qualitative determination is, confirmation of the diagnosis, all trans-retinoic acid and trioxide arsenic treatment response prediction and treatment efficacy knowledge in addition to minimal residual disease (MRD) monitoring and ability to identify relapse at early ages.


[ Türkçe Özet ] [ Tam Metin ] [ PDF ] [ Benzer Makaleler ]