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Ege Journal of Medicine
2017, Cilt 56, Sayı 2, Sayfa(lar) 077-081
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The investigation of effects of tyrosine kinase inhibitor on testicular tissue in terms of pluripotent capacity and cell adhesion in experimental diabetic mice
Kaan Özdedeli1, Hüseyin Aktuğ2, Fatih Oltulu2, Gülperi Öktem2, Altuğ Yavaşoğlu2, Eda Açıkgöz3, Gürkan Yiğittürk2, Kenan Demir2, Ayşegül Uysal2
1Trakya Üniversitesi Tıp Fakültesi, Üroloji Anabilim Dalı, Edirne, Türkiye
2Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye
3Yüzüncüyıl Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Van, Türkiye
Keywords: Tyrosine kinase inhibition, diyabetes mellitus, testis, experimental study

Aim: The aim of the study is to investigate the effects of tyrosine kinase inhibition on testicular tissue under the effects of diabetes mellitus.

Materials and Methods: Thirty one CD-1 male mice were divided into four groups: Group 1 (control group) consited 7, Group 2 (tyrosine kinase inhibitor given group) 7, Group 3 (diabetic group treated with SF) 8 and Group 4 (diabetic group treated with tyrosine kinase inhibitor) 9 experimental mice. No application was made to the animals in Group 1. In Group 2, mice were given tyrosine kinase inhibitor for 3 weeks. Diabetes was induced by intraperitoneal injection of single dose of streptozotocin 0.1mol/L. Mice with 250 mg/dL and higher blood glucose levels were accepted as diabetic. After waiting for 1 week, experimental diabetic mice in Group 3 were treated with SF and those in Group 4 were treated with tyrosine kinase inhibitor for 3 weeks. Then, all the experimental animals were sacrified and the testicular tissue was removed for histopathological examination. The one-way analysis of variance (ANOVA) is used for statistical analysis, a p value less than 0.05 was considered statistically significant.

Results: Histopathologic examination of the testes revealed loss of germ cells series in the seminiferous tubules and deterioration in cellular integrity depending on experimental diabetes.

Conclusion: By the study, it was demonstrated that diabetes lead to a decrease in the number of germ cells in testes and deterioration in cell adhesion mechanism. The administration of tyrosine kinase inhibitor could be considered to have a regenerative effect in this damage. The degree of treatment of this injury varies depending to the dose and time of the tyrosine kinase inhibitors. But, for the use of tyrosine kinase inhibitors in clinical practice of diabetes mellitus, more molecular studies are needed.


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