In this study, the effects of p97/VCP and its interacting partners Ufd1 and Npl4 proteins, which function at the retrotranslocation step of endoplasmic reticulum-associated degradation were investigated on the NF-κB pathway in the prostate cancer cell line.
Materials and Methods: The expressions of p97/VCP, Ufd1 and Npl4 were silenced in LNCaP cells using RNAi (RNA interference) technology. NF-κB activity was evaluated by dual luciferase assay system and the expression of NF-κB pathway proteins were investigated using immunoblotting.
Results: Silencing of expression of p97/VCP, Ufd1 and Npl4 significantly diminished NF-κB activity and increased the level of IκBα protein while decreased phosphorylated NF-κB and phosphorylated IκBα levels in LNCaP cells.
Conclusion: The decrease NF-κB activity upon silencing the expressions of retrotranslocation complex members in LNCaP cells might be associated with the increased level of NF-κB inhibitor IκBα. Our data suggests that p97/VCP and its interacting partners might be important factors on the regulation of NF-κB pathway in the prostate cancer.