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Ege Journal of Medicine
2018, Cilt 57, Sayı 3, Sayfa(lar) 142-146
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Investigating the effect of p97/VCP and Ufd1-Npl4 proteins functioning in the endoplasmic reticulum-associated degradation on the NF-κB pathway
Petek Ballar Kırmızıbayrak1, Burcu Erbaykent Tepedelen2
1Ege Üniversitesi Eczacılık Fakültesi, Biyokimya Anabilim Dalı, İzmir, Türkiye
2Uludağ Üniversitesi Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Bursa, Türkiye
Keywords: Endoplasmic reticulum-associated degradation, retrotranslocation, p97/VCP, NF-κB, prostate cancer

Aim: In this study, the effects of p97/VCP and its interacting partners Ufd1 and Npl4 proteins, which function at the retrotranslocation step of endoplasmic reticulum-associated degradation were investigated on the NF-κB pathway in the prostate cancer cell line.

Materials and Methods: The expressions of p97/VCP, Ufd1 and Npl4 were silenced in LNCaP cells using RNAi (RNA interference) technology. NF-κB activity was evaluated by dual luciferase assay system and the expression of NF-κB pathway proteins were investigated using immunoblotting.

Results: Silencing of expression of p97/VCP, Ufd1 and Npl4 significantly diminished NF-κB activity and increased the level of IκBα protein while decreased phosphorylated NF-κB and phosphorylated IκBα levels in LNCaP cells.

Conclusion: The decrease NF-κB activity upon silencing the expressions of retrotranslocation complex members in LNCaP cells might be associated with the increased level of NF-κB inhibitor IκBα. Our data suggests that p97/VCP and its interacting partners might be important factors on the regulation of NF-κB pathway in the prostate cancer.


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