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Ege Journal of Medicine
2018, Cilt 57, Sayı 3, Sayfa(lar) 157-162
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Significant increase in ZNF304 and decrease in CXCR4 gene expressions may alter anoikis in prostate cancer
Şule Ayla1, Gülperi Öktem2, Cüneyd Parlayan3
1İstanbul Medipol Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER), İstanbul, Türkiye
2Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye
3İstanbul Medipol Üniversitesi Doğa Bilimleri ve Mühendislik Fakültesi, Biyomedikal Mühendislik Anabilim Dalı, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER), İstanbul, Türkiye
Keywords: Apoptosis, anoikis, cancer, mesenchymal transition

Aim: To analyze genes that may increase or inhibit the anoikis mechanism between prostate cancer cell line and prostate normal epithelial cell line and examine the possible role of cancer in cancer development.

Materials and Methods: Human prostate epithelial cell line (RWPE) and prostate cancer cell line (DU145) were acquired from ATCC. Both cell lines were maintanied in RPMI 1640 (Biological Industries) medium. Total RNA were isolated and fragmented. Adapters were ligated to prepare RNA library for whole trasncriptome experiments. Statistics and bioinformatics analysis including mapping, clustering, sequencing were done by using Genomics Workbench v 8. (Qiagen) software.

Results: As we compared the normal prostate ephitalial cells (RWPE) and prosatate cancer cells (DU 145); ZNF304,PYCARD ve NOTCH3 were significantly (p<0.05) up-regulated in DU145 cells, on the other hand, CXCR4, PAK3, SERPINB1 genes were significantly down-regulated.

Conclusion: We found that there are significant differential gene expressions in DU-145 cells which may lead to metastatic state via evasion of anoikis process.


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