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Ege Journal of Medicine
2019, Cilt 58, Sayı 1, Sayfa(lar) 032-038
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Investigation of the effects of zoledronic acid in breast cancer stem cell line (MCF 7) for autophagic gene level
Fatih Oltulu1, Ayşegül Uysal1, Hadi Rouhrazi2, Kubilay Doğan Kılıç1, Duygu Çalık Kocatürk1, Gülperi Öktem1
1Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye
2Ege Üniversitesi Sağlık Bilimleri Enstitüsü, Tıbbi Biyokimya Anabilim Dalı, İzmir, Türkiye
Keywords: Autophagy, cancer stem cell, zoleodronic acid, TNF, heat shock protein (HSP90)

Aim: In this study we examined differences in the expression of genes associated with autophagy and CSCs for the treatment ZA applied via taking breast cancer stem cells as models.

Materials and Methods: CSCs with CD44high/CD24high surface markers have been isolated from the MCF-7 breast cancer cell line with flow cytometry methods. cDNA was synthesized for ZA treatment applied and not applied CSC group with total RNA isolation. Then, 41 essential autophagy-related gene expression levels were analyzed by qRT-PCR array analysis.

Results: When ZA treatment administrated group compared with non-treatment group, downregulation of TGM2, TNF, HSPA8 and upregulation of HSP90AA1 was detected.

Conclusion: There are differences of expression profiles of genes related to autophagy between ZA treated and non-ZA tread group. These findings may provide important information towards us to target CSCs to develop more effective treatments.


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