Yıl 2021, Cilt 60 , Sayı 1, Sayfalar 39 - 50 2021-03-31

Effect of ferulic acid on cytokine release in human leukemia monocytic cells induced with lipopolysaccharides
Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi

Şahin ÖZTÜRK [1] , Burak DURMAZ [2] , Hikmet MEMMEDOV [3] , Latife Merve OKTAY [4] , Selvi Nur GÜNEL [5] , Murat OLUKMAN [6] , Eser Yıldırım SÖZMEN [7]


Aim: Macrophages are accepted as cells that initially contact with the pathogens and initiate the innate immune response. They play effective roles in innate immune and inflammatory responses by intercellular relations and inflammatory mediator secretion. Human THP-1 leukemia monocytic cells are frequently used for the in vitro determination of the signal pathways, and the functions of macrophages. Lipopolysaccharide is commonly used to induce macrophage differentiation of monocytic cell lines but the extent of differentiation in comparison to primary tissue macrophages is unclear. Ferulic acid (FA) is known to inhibit the expression and / or activity of proinflammatory cytokines, including inducible nitric oxide synthase, caspases, and cyclooxygenase (COX)-2. In this study, we aimed to investigate the effect of Ferulic acid on cytokine (COX-1, IL-1α, IL-1β, TNF-α, IL-6, IL-10, NF-kβ and IFN-γ) levels in lipopolysaccharide induced THP-1 monocyte cells. Materials and Methods: TNF alpha levels were measured by performing applications at different concentrations and times, and the most appropriate concentration and duration were determined. Cytokine levels of COX-1, IL-1α, IL-1β, TNF-α, IL-6, IL-10, NF-kβ and IFN-γ were measured by ELISA in supernatants collected at the end of incubation times from wells. Results: We have found that ferulic acid inhibits the expression of TNF-α, IL-1α and IL-1β in LPS-induced macrophages by inhibition of NF-kB. Conclusion: It has been found that the effect of ferulic acid on cytokine release in LPS-induced THP-1 cells may be effective in the treatment of excessive inflammatory response and protection against auto-immune diseases.
Amaç: Makrofajlar doğal immün cevabı başlatan ve patojenle ilk temasta bulunan hücreler olarak kabul edilmektedir. Hem hücreler arası ilişkiler hem de inflamatuar mediyatörlerin salınımı yoluyla doğal immün ve inflamatuar yanıtta etkin rol oynamaktadırlar. İnsan THP-1 lösemi monositik hücreleri, makrofajların in vitro olarak fonksiyonlarını, mekanizmalarını ve sinyal yollarını araştırmak için en çok kullanılan hücre dizisidir. Lipopolisakkarit makrofaj farklılaşmasını başlatmak için yaygın olarak kullanılan uyarıcılar arasında yer almaktadır. Ferulik asidin, indüklenebilir nitrik oksit sentaz, kaspazlar ve siklooksijenaz (COX)-2 dahil olmak üzere proinflamatuar sitokinlerin ekspresyonunu ve/veya aktivitesini inhibe ettiği bilinmektedir. Bu çalışmada, lipopolisakkarit ile indüklenen THP-1 monosit hücrelerinde Ferulik asidin sitokin (COX-1, IL-lα, IL-1β, TNF-α, IL-6, IL-10, NF-kβ ve IFN-y) düzeyleri üzerindeki etkisini araştırmayı amaçladık. Gereç ve Yöntem: TNF alfa seviyeleri farklı konsantrasyonlarda ve zamanlarda LPS eklenerek ölçüldü ve en uygun konsantrasyon ve süre belirlendi. COX-1, IL-lα, IL-lβ, TNF-α, IL-6, IL-10, NF-kβ ve IFN-y'nin sitokin miktarları, inkübasyon sürelerinin sonunda toplanan süpernatanlarda ELISA ile ölçüldü. Bulgular: Ferulik asidin, NF-kβ inhibisyonu ile lipopolisakkarit ile indüklenen THP-1 hücrelerinde artan TNF-α, IL-lα ve IL-lβ ekspresyonunu inhibe ettiğini bulduk. Sonuç: Ferulik asidin LPS ile indüklenen THP-1 hücrelerinde sitokin salınımı üzerindeki etkisinin gösterilmesi, aşırı inflamatuar yanıtın tedavisinde ve oto-immün hastalıklara karşı korunmada etkili olabileceğini düşündürdü.
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Birincil Dil tr
Konular Sağlık Bilimleri ve Hizmetleri
Bölüm Araştırma Makaleleri
Yazarlar

Orcid: 0000-0002-5793-4993
Yazar: Şahin ÖZTÜRK
Kurum: Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Orcid: 0000-0002-5285-3641
Yazar: Burak DURMAZ (Sorumlu Yazar)
Kurum: Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Orcid: 0000-0002-8012-0445
Yazar: Hikmet MEMMEDOV
Kurum: Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Orcid: 0000-0002-7110-3379
Yazar: Latife Merve OKTAY
Kurum: Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Orcid: 0000-0003-0612-2263
Yazar: Selvi Nur GÜNEL
Kurum: Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Orcid: 0000-0002-9868-4716
Yazar: Murat OLUKMAN
Kurum: Ege Üniversitesi Tıp Fakültesi, Farmakoloji Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Orcid: 0000-0002-6383-6724
Yazar: Eser Yıldırım SÖZMEN
Kurum: Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, İzmir, Türkiye
Ülke: Turkey


Tarihler

Başvuru Tarihi : 20 Ekim 2020
Kabul Tarihi : 30 Kasım 2020
Yayımlanma Tarihi : 31 Mart 2021

Bibtex @araştırma makalesi { etd887360, journal = {Ege Tıp Dergisi}, issn = {1016-9113}, eissn = {2147-6500}, address = {}, publisher = {Ege Üniversitesi}, year = {2021}, volume = {60}, pages = {39 - 50}, doi = {10.19161/etd.887360}, title = {Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi}, key = {cite}, author = {Öztürk, Şahin and Durmaz, Burak and Memmedov, Hikmet and Oktay, Latife Merve and Günel, Selvi Nur and Olukman, Murat and Sözmen, Eser Yıldırım} }
APA Öztürk, Ş , Durmaz, B , Memmedov, H , Oktay, L , Günel, S , Olukman, M , Sözmen, E . (2021). Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi . Ege Tıp Dergisi , 60 (1) , 39-50 . DOI: 10.19161/etd.887360
MLA Öztürk, Ş , Durmaz, B , Memmedov, H , Oktay, L , Günel, S , Olukman, M , Sözmen, E . "Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi" . Ege Tıp Dergisi 60 (2021 ): 39-50 <http://egetipdergisi.com.tr/tr/pub/issue/60217/887360>
Chicago Öztürk, Ş , Durmaz, B , Memmedov, H , Oktay, L , Günel, S , Olukman, M , Sözmen, E . "Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi". Ege Tıp Dergisi 60 (2021 ): 39-50
RIS TY - JOUR T1 - Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi AU - Şahin Öztürk , Burak Durmaz , Hikmet Memmedov , Latife Merve Oktay , Selvi Nur Günel , Murat Olukman , Eser Yıldırım Sözmen Y1 - 2021 PY - 2021 N1 - doi: 10.19161/etd.887360 DO - 10.19161/etd.887360 T2 - Ege Tıp Dergisi JF - Journal JO - JOR SP - 39 EP - 50 VL - 60 IS - 1 SN - 1016-9113-2147-6500 M3 - doi: 10.19161/etd.887360 UR - https://doi.org/10.19161/etd.887360 Y2 - 2020 ER -
EndNote %0 Ege Tıp Dergisi Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi %A Şahin Öztürk , Burak Durmaz , Hikmet Memmedov , Latife Merve Oktay , Selvi Nur Günel , Murat Olukman , Eser Yıldırım Sözmen %T Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi %D 2021 %J Ege Tıp Dergisi %P 1016-9113-2147-6500 %V 60 %N 1 %R doi: 10.19161/etd.887360 %U 10.19161/etd.887360
ISNAD Öztürk, Şahin , Durmaz, Burak , Memmedov, Hikmet , Oktay, Latife Merve , Günel, Selvi Nur , Olukman, Murat , Sözmen, Eser Yıldırım . "Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi". Ege Tıp Dergisi 60 / 1 (Mart 2021): 39-50 . https://doi.org/10.19161/etd.887360
AMA Öztürk Ş , Durmaz B , Memmedov H , Oktay L , Günel S , Olukman M , Sözmen E . Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi. ETD. 2021; 60(1): 39-50.
Vancouver Öztürk Ş , Durmaz B , Memmedov H , Oktay L , Günel S , Olukman M , Sözmen E . Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi. Ege Tıp Dergisi. 2021; 60(1): 39-50.
IEEE Ş. Öztürk , B. Durmaz , H. Memmedov , L. Oktay , S. Günel , M. Olukman ve E. Sözmen , "Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi", Ege Tıp Dergisi, c. 60, sayı. 1, ss. 39-50, Mar. 2021, doi:10.19161/etd.887360