Öz
Amaç: Düşükler günümüzde giderek daha önemli bir sorun haline gelmektedir. Üreme çağındaki kadınlarda ilk trimester gebelik kaybının sıklığı artmaktadır. Bu çalışmada, ilk trimester gebelik kayıpları ile PON1 ve ARY düzeyleri arasındaki olası ilişkinin gösterilmesi amaçlanmıştır.
Gereçler ve Yöntem: 6-14 gebelik haftasında 25 gebe sağlıklı kadın (Grup 1) ve ilk trimester düşüklü (Grup 2) 25 kadın çalışmaya dahil edildi. Serum örneklerinde paraoksonaz 1 ve arilesteraz aktiviteleri, tam otomatik kolorimetrik yöntemler kullanılarak araştırıldı. Grupları karşılaştırmak için ANOVA testi kullanıldı.
Bulgular: PON1 ve ARY aktiviteleri, ilk trimesterde düşük yapan gebelerde Anova testi ile analiz edilen ilk trimesterdeki sağlıklı gebe kadınlara göre istatistiksel olarak anlamlı derecede düşüktür (p <0,05).
Sonuç: Bu çalışmanın sonuçları, ilk trimester gebelik kayıplarında paraoksonaz 1 ve arilesteraz antioksidan enzim aktivitelerinin anlamlı prediktif klinik laboratuvar parametreleri olarak değerlendirilebildiğini göstermiştir. Bu çalışma daha büyük hasta gruplarıyla doğrulanırsa, araştırma sonuçlarımız değer kazanacaktır.
Anahtar Kelimeler
Destekleyen Kurum
-
Proje Numarası
-
Teşekkür
-
Kaynakça
- 1. Fang J, Xie B, Chen B, Qiao C, Zheng B, Luan X, Liu J, Yan Y, Zheng Q, Wang M, Chen W, He Z, Shen C, Li H, Chen X, Yu J. Biochemical clinical factors associated with missed abortion independent of maternal age: A retrospective study of 795 cases with missed abortion and 694 cases with normal pregnancy. Medicine (Baltimore). 2018;97(50):e13573. doi: 10.1097/MD.0000000000013573.
- 2. Akkaya H, Uysal G, Büke B, Gök G, Erel Ö, Karakükçü Ç. Evaluation of thiol/disulphide homeostasis as a novel predictor testing tool of early pregnancy viability. Taiwan J Obstet Gynecol. 2018;57(3):427-431. doi: 10.1016/j.tjog.2018.04.034.
- 3. Gültekin F, Nazıroğlu M, Savaş HB, Çiğ B. Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats. Metab Brain Dis. 2018;33(5):1761-1774. doi: 10.1007/s11011-018-0289-0.
- 4. Savaş HB, Yüksel Ö, Şanlidere Aloğlu H, Öner Z, Demir Özer E, Gultekin F. Effects of food based yeast supplementation on oxidative stress in rats fed by high cholesterol diet. Cell Membranes and Free Radical Research. 5:3 2013; 252-255.
- 5. Seflek HN, Kalkan S, Cuce G, Kılınc I, Sozen ME. Effects of Nigella sativa oil on ovarian volume, oxidant systems, XIAP and NF-kB expression in an experimental model of diabetes. Biotech Histochem. 2019;94(5):325-333. doi: 10.1080/10520295.2019.1566571.
- 6. Cuce G, Canbaz HT, Sozen ME, Yerlikaya FH, Kalkan S. Vitamin E and selenium treatment of monocrotaline induced hepatotoxicity in rats. Biotech Histochem. 2017;92(1):59-67. doi: 10.1080/10520295.2016.1267798.
- 7. Savas HB, Gultekin F, Ciris İM. Positive effects of meal frequency and calorie restriction on antioxidant systems in rats. North Clin Istanbul. 2017;4(2):109–116. doi: 10.14744/nci.2017.21548.
- 8. Savas HB. Klinik Biyokimyada Kanıta Dayalı Yaklaşım. 1. Edition. Ankara: Akademisyen Publishing, 2019, p: 20-32.
- 9. Bednarz-Misa I, Berdowska I, Zboch M, Misiak B, Zieliński B, Płaczkowska S, Fleszar M, Wiśniewski J, Gamian A, Krzystek-Korpacka M. Paraoxonase 1 decline and lipid peroxidation rise reflect a degree of brain atrophy and vascular impairment in dementia. Adv Clin Exp Med. 2020;29(1):71-78. doi: 10.17219/acem/111377.
- 10. Ayan D, Şeneş M, Çaycı AB, Söylemez S, Eren N, Altuntaş Y, Öztürk FY. Evaluation of Paraoxonase, Arylesterase, and Homocysteine Thiolactonase Activities in Patients with Diabetes and Incipient Diabetes Nephropathy. J Med Biochem. 2019;26;38(4):481-488. doi: 10.2478/jomb-2019-0014.
- 11. Savran M, Aslankoc R, Ozmen O, Erzurumlu Y, Savas HB, Temel EN, Kosar PA, Boztepe S. Agomelatine could prevent brain and cerebellum injury against LPS-induced neuroinflammation in rats. Cytokine. 2019;20;127:154957. doi: 10.1016/j.cyto.2019.154957.
- 12. Savran M, Asci H, Ozmen O, Erzurumlu Y, Savas HB, Sonmez Y, Sahin Y. Melatonin protects the heart and endothelium against high fructose corn syrup consumption-induced cardiovascular toxicity via SIRT-1 signaling. Hum Exp Toxicol. 2019;30:960327119860188. doi: 10.1177/0960327119860188.
- 13. Savran M, Ozmen O, Erzurumlu Y, Savas HB, Asci S, Kaynak M. The Impact of Prophylactic Lacosamide on LPS-Induced Neuroinflammation in Aged Rats. Inflammation. 2019;42(5):1913-1924. doi: 10.1007/s10753-019-01053-7.
- 14. Öztürk E, Pehlivan S, Ozcan C, Ugur MG, Balat O. Paraoxonase-1 Polymorphisms (L55M/Q192R) and Activities (PONase/AREase) in Patients with Idiopathic Recurrent Early Pregnancy Loss: A Preliminary Study. Genet Test Mol Biomarkers. 2019;23(7):501-505. doi: 10.1089/gtmb.2018.0245.
- 15. Toy H, Camuzcuoglu H, Celik H, Erel O, Aksoy N. Assessment of serum paraoxonase and arylesterase activities in early pregnancy failure. Swiss Med Wkly. 2009;7;139(5-6):76-81. doi: smw-12495.
- 16. Dikbas L, Dur R, Tas İH, Yapça ÖE, Dikbas N. Low transcriptional activity of PON2 in recurrent abortion: A novel therapeutic agent? J Gynecol Obstet Hum Reprod. 2018;47(8):379-383. doi: 10.1016/j.jogoh.2018.06.006.
- 17. Sami AS, Suat E, Alkis I, Karakus Y, Guler S. The role of trace element, mineral, vitamin and total antioxidant status in women with habitual abortion. J Matern Fetal Neonatal Med. 2019;1-8. doi: 10.1080/14767058.2019.1623872.
Öz
Aim: Miscarriages are becoming increasingly important problem nowadays. First trimester pregnancy loss frequency in women of reproductive age is increasing. In this study, it is aimed to show the possible relationship between first trimester pregnancy losses and PON1 and ARY activities.
Material and Method: 25 pregnant healthy women (Group 1) and 25 women with first trimester miscarriage (Group 2) in 6-14 gestational weeks were included in this study. Paraoxonase 1 and arylesterase activities of the sera samples were investigated using fully automated colorimetric methods. ANOVA test was used to compare the groups.
Results: PON1 and ARY activities are statistically significantly lower in pregnant women with first trimester miscarriage compared to healthy pregnant women in first trimester analyzed by Anova test (p<0.05).
Conclusion: The results of this study showed that paraoxonase 1 and arylesterase antioxidant enzyme activities can be evaluated as significant predictive clinical laboratory parameters in first trimester pregnancy losses. If this study is confirmed with larger patient groups, our research results will gain more value.
Anahtar Kelimeler
Pregnants first trimester miscarriage paraoxonase 1 arylesterase
Proje Numarası
-
Kaynakça
- 1. Fang J, Xie B, Chen B, Qiao C, Zheng B, Luan X, Liu J, Yan Y, Zheng Q, Wang M, Chen W, He Z, Shen C, Li H, Chen X, Yu J. Biochemical clinical factors associated with missed abortion independent of maternal age: A retrospective study of 795 cases with missed abortion and 694 cases with normal pregnancy. Medicine (Baltimore). 2018;97(50):e13573. doi: 10.1097/MD.0000000000013573.
- 2. Akkaya H, Uysal G, Büke B, Gök G, Erel Ö, Karakükçü Ç. Evaluation of thiol/disulphide homeostasis as a novel predictor testing tool of early pregnancy viability. Taiwan J Obstet Gynecol. 2018;57(3):427-431. doi: 10.1016/j.tjog.2018.04.034.
- 3. Gültekin F, Nazıroğlu M, Savaş HB, Çiğ B. Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats. Metab Brain Dis. 2018;33(5):1761-1774. doi: 10.1007/s11011-018-0289-0.
- 4. Savaş HB, Yüksel Ö, Şanlidere Aloğlu H, Öner Z, Demir Özer E, Gultekin F. Effects of food based yeast supplementation on oxidative stress in rats fed by high cholesterol diet. Cell Membranes and Free Radical Research. 5:3 2013; 252-255.
- 5. Seflek HN, Kalkan S, Cuce G, Kılınc I, Sozen ME. Effects of Nigella sativa oil on ovarian volume, oxidant systems, XIAP and NF-kB expression in an experimental model of diabetes. Biotech Histochem. 2019;94(5):325-333. doi: 10.1080/10520295.2019.1566571.
- 6. Cuce G, Canbaz HT, Sozen ME, Yerlikaya FH, Kalkan S. Vitamin E and selenium treatment of monocrotaline induced hepatotoxicity in rats. Biotech Histochem. 2017;92(1):59-67. doi: 10.1080/10520295.2016.1267798.
- 7. Savas HB, Gultekin F, Ciris İM. Positive effects of meal frequency and calorie restriction on antioxidant systems in rats. North Clin Istanbul. 2017;4(2):109–116. doi: 10.14744/nci.2017.21548.
- 8. Savas HB. Klinik Biyokimyada Kanıta Dayalı Yaklaşım. 1. Edition. Ankara: Akademisyen Publishing, 2019, p: 20-32.
- 9. Bednarz-Misa I, Berdowska I, Zboch M, Misiak B, Zieliński B, Płaczkowska S, Fleszar M, Wiśniewski J, Gamian A, Krzystek-Korpacka M. Paraoxonase 1 decline and lipid peroxidation rise reflect a degree of brain atrophy and vascular impairment in dementia. Adv Clin Exp Med. 2020;29(1):71-78. doi: 10.17219/acem/111377.
- 10. Ayan D, Şeneş M, Çaycı AB, Söylemez S, Eren N, Altuntaş Y, Öztürk FY. Evaluation of Paraoxonase, Arylesterase, and Homocysteine Thiolactonase Activities in Patients with Diabetes and Incipient Diabetes Nephropathy. J Med Biochem. 2019;26;38(4):481-488. doi: 10.2478/jomb-2019-0014.
- 11. Savran M, Aslankoc R, Ozmen O, Erzurumlu Y, Savas HB, Temel EN, Kosar PA, Boztepe S. Agomelatine could prevent brain and cerebellum injury against LPS-induced neuroinflammation in rats. Cytokine. 2019;20;127:154957. doi: 10.1016/j.cyto.2019.154957.
- 12. Savran M, Asci H, Ozmen O, Erzurumlu Y, Savas HB, Sonmez Y, Sahin Y. Melatonin protects the heart and endothelium against high fructose corn syrup consumption-induced cardiovascular toxicity via SIRT-1 signaling. Hum Exp Toxicol. 2019;30:960327119860188. doi: 10.1177/0960327119860188.
- 13. Savran M, Ozmen O, Erzurumlu Y, Savas HB, Asci S, Kaynak M. The Impact of Prophylactic Lacosamide on LPS-Induced Neuroinflammation in Aged Rats. Inflammation. 2019;42(5):1913-1924. doi: 10.1007/s10753-019-01053-7.
- 14. Öztürk E, Pehlivan S, Ozcan C, Ugur MG, Balat O. Paraoxonase-1 Polymorphisms (L55M/Q192R) and Activities (PONase/AREase) in Patients with Idiopathic Recurrent Early Pregnancy Loss: A Preliminary Study. Genet Test Mol Biomarkers. 2019;23(7):501-505. doi: 10.1089/gtmb.2018.0245.
- 15. Toy H, Camuzcuoglu H, Celik H, Erel O, Aksoy N. Assessment of serum paraoxonase and arylesterase activities in early pregnancy failure. Swiss Med Wkly. 2009;7;139(5-6):76-81. doi: smw-12495.
- 16. Dikbas L, Dur R, Tas İH, Yapça ÖE, Dikbas N. Low transcriptional activity of PON2 in recurrent abortion: A novel therapeutic agent? J Gynecol Obstet Hum Reprod. 2018;47(8):379-383. doi: 10.1016/j.jogoh.2018.06.006.
- 17. Sami AS, Suat E, Alkis I, Karakus Y, Guler S. The role of trace element, mineral, vitamin and total antioxidant status in women with habitual abortion. J Matern Fetal Neonatal Med. 2019;1-8. doi: 10.1080/14767058.2019.1623872.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Kadın Hastalıkları ve Doğum |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Proje Numarası | - |
| Yayımlanma Tarihi | 1 Temmuz 2021 |
| Gönderilme Tarihi | 23 Ağustos 2020 |
| Kabul Tarihi | 19 Şubat 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 18 Sayı: 2 |
