Araştırma Makalesi
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Hidrojen sülfür, karbon monoksit ve nitrik oksidin sıçanlarda pentilentetrazol indüklü nöbetler üzerindeki etkileri

Yıl 2020, Cilt: 15 Sayı: 3, 1 - 6, 16.10.2020
https://doi.org/10.17517/ksutfd.681282

Öz

Amaç: Sıçanlarda pentilentetrazol indüklü konvülsiyonlarda biyoaktif gaz mediyatörler hidrojen sülfür (H2S), karbon monoksid (CO) ve nitrik oksid (NO)'in anti-konvülsan etkilerinin araştırılması amaçlanmıştır.
Gereç Yöntem: Seksen erkek Wistar-Albino sıçan rastgele on gruba ayrılmıştır. Konvülsiyonlar, intraperitonal olarak verilen 60 mg/kg pentilentetrazol ile indüklendi. Pentilentetrazolden 60 dakika sonra Diazepam, NaHS, CORM-2, L-arginin ve L-NAME uygulandı. Nöbet latensi, nöbet süresi ve nöbet skoru değerlendirildi. Veriler Kruskal Wallis testi ve ardından Tukey ile analiz edildi. P <0.05 istatistiksel olarak anlamlı kabul edildi.
Bulgular: Diazepam (2 mg/kg) nöbet latensini uzattı, diğer gruplarda ise anlamlı bir fark bulunmadı. Diazepam (1 ve 2 mg/kg) ve NaHS (10 mg/kg) nöbet süresini azalttı. NaHS (5 mg/kg), CORM-2 (5 ve 10 mg/kg), L-Arjinin (30 ve 100 mg / kg) ve L-NAME (30 mg / kg), nöbet skorunu diazepam ile karşılaştırıldığında önemli ölçüde artırdı.
Sonuç: NaHS (10 mg/kg) pentilentetrazole bağlı nöbetlerde koruyucu olabilir.

Destekleyen Kurum

Eskişehir Osmangazi Üniversitesi Tıp Fakültesi Bilimsel Araştırma Projeleri Komisyonu

Proje Numarası

2019/11D05

Kaynakça

  • 1. Stafstrom CE, Carmant L. Seizures and epilepsy: an overview for neuroscientists. Cold Spring Harb Perspect Med. 2015;5(6).
  • 2. Singh A, Trevick S. The Epidemiology of Global Epilepsy. Neurol Clin. 2016;34(4):837-47.
  • 3. Laxer KD, Trinka E, Hirsch LJ, Cendes F, Langfitt J, Delanty N, et al. The consequences of refractory epilepsy and its treatment. Epilepsy Behav. 2014;37:59-70.
  • 4. Newton RW. When is drug treatment not necessary in epilepsy? Factors that should influence the decision to prescribe. J R Soc Med. 2004;97(1):15-9.
  • 5. Lazarini-Lopes W, Do Val-da Silva RA, da Silva-Junior RMP, Leite JP, Garcia-Cairasco N. The anticonvulsant effects of cannabidiol in experimental models of epileptic seizures: from behavior and mechanisms to clinical insights. Neurosci Biobehav Rev. 2020.
  • 6. Johannessen SI, Landmark CJ. Antiepileptic drug interactions - principles and clinical implications. Curr Neuropharmacol. 2010;8(3):254-67.
  • 7. Loscher W. Critical review of current animal models of seizures and epilepsy used in the discovery and development of new antiepileptic drugs. Seizure. 2011;20(5):359-68.
  • 8. Samokhina E, Samokhin A. Neuropathological profile of the pentylenetetrazol (PTZ) kindling model. Int J Neurosci. 2018;128(11):1086-96.
  • 9. Li B, Wang L, Sun Z, Zhou Y, Shao D, Zhao J, et al. The anticonvulsant effects of SR 57227 on pentylenetetrazole-induced seizure in mice. PLoS One. 2014;9(4):e93158.
  • 10. Farrugia G, Szurszewski JH. Carbon monoxide, hydrogen sulfide, and nitric oxide as signaling molecules in the gastrointestinal tract. Gastroenterology. 2014;147(2):303-13.
  • 11. Shefa U, Yeo SG, Kim MS, Song IO, Jung J, Jeong NY, et al. Role of Gasotransmitters in Oxidative Stresses, Neuroinflammation, and Neuronal Repair. Biomed Res Int. 2017;2017:1689341.
  • 12. Shefa U, Kim D, Kim MS, Jeong NY, Jung J. Roles of Gasotransmitters in Synaptic Plasticity and Neuropsychiatric Conditions. Neural Plast. 2018;2018:1824713.
  • 13. Yang ZX, Qin J, Du JB, Chang XZ, Han Y. [Effect of endogenous heme oxygenase-carbon monoxide on brain damage induced by recurrent febrile seizures]. Zhonghua Er Ke Za Zhi. 2005;43(4):252-5.
  • 14. Luo Y, Wu PF, Zhou J, Xiao W, He JG, Guan XL, et al. Aggravation of seizure-like events by hydrogen sulfide: involvement of multiple targets that control neuronal excitability. CNS Neurosci Ther. 2014;20(5):411-9.
  • 15. Herberg LJ, Grottick A, Rose IC. Nitric oxide synthesis, epileptic seizures and kindling. Psychopharmacology (Berl). 1995;119(1):115-23.
  • 16. Osonoe K, Mori N, Suzuki K, Osonoe M. Antiepileptic effects of inhibitors of nitric oxide synthase examined in pentylenetetrazol-induced seizures in rats. Brain Res. 1994;663(2):338-40.
  • 17. Buisson A, Lakhmeche N, Verrecchia C, Plotkine M, Boulu RG. Nitric oxide: an endogenous anticonvulsant substance. Neuroreport. 1993;4(4):444-6. 18. de Oliveira CC, de Oliveira CV, Grigoletto J, Ribeiro LR, Funck VR, Grauncke AC, et al. Anticonvulsant activity of beta-caryophyllene against pentylenetetrazol-induced seizures. Epilepsy Behav. 2016;56:26-31.
  • 19. Kang X, Shen X, Xia Y. Electroacupuncture-induced attenuation of experimental epilepsy: a comparative evaluation of acupoints and stimulation parameters. Evid Based Complement Alternat Med. 2013;2013:149612.
  • 20. Dhir A, Rogawski MA. Determination of minimal steady-state plasma level of diazepam causing seizure threshold elevation in rats. Epilepsia. 2018;59(5):935-44.
  • 21. Polhemus DJ, Lefer DJ. Emergence of hydrogen sulfide as an endogenous gaseous signaling molecule in cardiovascular disease. Circ Res. 2014;114(4):730-7.
  • 22. Panthi S, Manandhar S, Gautam K. Hydrogen sulfide, nitric oxide, and neurodegenerative disorders. Transl Neurodegener. 2018;7:3.
  • 23. Leffler CW, Parfenova H, Jaggar JH, Wang R. Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation. J Appl Physiol (1985). 2006;100(3):1065-76.
  • 24. Hu LF, Lu M, Hon Wong PT, Bian JS. Hydrogen sulfide: neurophysiology and neuropathology. Antioxid Redox Signal. 2011;15(2):405-19.
  • 25. Kimura H, Nagai Y, Umemura K, Kimura Y. Physiological roles of hydrogen sulfide: synaptic modulation, neuroprotection, and smooth muscle relaxation. Antioxid Redox Signal. 2005;7(5-6):795-803.
  • 26. Abe K, Kimura H. The possible role of hydrogen sulfide as an endogenous neuromodulator. J Neurosci. 1996;16(3):1066-71.
  • 27. Tang G, Wu L, Wang R. Interaction of hydrogen sulfide with ion channels. Clin Exp Pharmacol Physiol. 2010;37(7):753-63.
  • 28. Otterbein LE. Carbon monoxide: innovative anti-inflammatory properties of an age-old gas molecule. Antioxid Redox Signal. 2002;4(2):309-19.
  • 29. Otterbein LE, Soares MP, Yamashita K, Bach FH. Heme oxygenase-1: unleashing the protective properties of heme. Trends Immunol. 2003;24(8):449-55.
  • 30. Fredenburgh LE, Merz AA, Cheng S. Haeme oxygenase signalling pathway: implications for cardiovascular disease. Eur Heart J. 2015;36(24):1512-8.
  • 31. Joshi HP, Kim SB, Kim S, Kumar H, Jo MJ, Choi H, et al. Nanocarrier-mediated Delivery of CORM-2 Enhances Anti-allodynic and Anti-hyperalgesic Effects of CORM-2. Mol Neurobiol. 2019;56(8):5539-54.
  • 32. Al-Huseini LM, Aw Yeang HX, Hamdam JM, Sethu S, Alhumeed N, Wong W, et al. Heme oxygenase-1 regulates dendritic cell function through modulation of p38 MAPK-CREB/ATF1 signaling. J Biol Chem. 2014;289(23):16442-51.
  • 33. Crespy D, Landfester K, Schubert US, Schiller A. Potential photoactivated metallopharmaceuticals: from active molecules to supported drugs. Chem Commun (Camb). 2010;46(36):6651-62.
  • 34. Queiroga CS, Vercelli A, Vieira HL. Carbon monoxide and the CNS: challenges and achievements. Br J Pharmacol. 2015;172(6):1533-45.
  • 35. Zimmermann A, Leffler CW, Tcheranova D, Fedinec AL, Parfenova H. Cerebroprotective effects of the CO-releasing molecule CORM-A1 against seizure-induced neonatal vascular injury. Am J Physiol Heart Circ Physiol. 2007;293(4):H2501-7.
  • 36. Banach M, Piskorska B, Czuczwar SJ, Borowicz KK. Nitric oxide, epileptic seizures, and action of antiepileptic drugs. CNS Neurol Disord Drug Targets. 2011;10(7):808-19.
  • 37. Jelenkovic A, Jovanovic M, Ninkovic M, Maksimovic M, Bokonjic D, Boskovic B. Nitric oxide (NO) and convulsions induced by pentylenetetrazol. Ann N Y Acad Sci. 2002;962:296-305.

The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats

Yıl 2020, Cilt: 15 Sayı: 3, 1 - 6, 16.10.2020
https://doi.org/10.17517/ksutfd.681282

Öz

Objective: It is aimed to investigate the anti-convulsant effects of bioactive gas mediators hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) in pentylenetetrazole-induced convulsions in rats.
Material Methods: Eighty male Wistar-Albino rats randomly divided into ten groups. Convulsions was induced by administering 60 mg/kg pentylentetrazole intraperitonally. Diazepam, NaHS, CORM-2, L-arginine and L-NAME were administred 60 minutes after pentylentetrazole. Seizure latency, seizure duration and seizure score were evaluated. Data were analyzed by the Kruskal Wallis test followed by posthoc analyses with Tukey. P <0.05 was considered statistically significant.
Results: Diazepam (2 mg/kg) extended the seizure latency and no significant difference was found in other groups. Diazepam (1 and 2 mg/kg) and NaHS (10 mg/kg) reduced seizure duration. NaHS (5 mg/kg), CORM-2 (5 and 10 mg/kg), L-arginine (30 and 100 mg/kg) and L-NAME (30 mg/kg) increased the seizure score significantly as compared to diazepam (1 and 2 mg/kg) groups.
Conclusion: NaHS (10 mg/kg) may be protective in pentylentetrazole-induced seizures.

Proje Numarası

2019/11D05

Kaynakça

  • 1. Stafstrom CE, Carmant L. Seizures and epilepsy: an overview for neuroscientists. Cold Spring Harb Perspect Med. 2015;5(6).
  • 2. Singh A, Trevick S. The Epidemiology of Global Epilepsy. Neurol Clin. 2016;34(4):837-47.
  • 3. Laxer KD, Trinka E, Hirsch LJ, Cendes F, Langfitt J, Delanty N, et al. The consequences of refractory epilepsy and its treatment. Epilepsy Behav. 2014;37:59-70.
  • 4. Newton RW. When is drug treatment not necessary in epilepsy? Factors that should influence the decision to prescribe. J R Soc Med. 2004;97(1):15-9.
  • 5. Lazarini-Lopes W, Do Val-da Silva RA, da Silva-Junior RMP, Leite JP, Garcia-Cairasco N. The anticonvulsant effects of cannabidiol in experimental models of epileptic seizures: from behavior and mechanisms to clinical insights. Neurosci Biobehav Rev. 2020.
  • 6. Johannessen SI, Landmark CJ. Antiepileptic drug interactions - principles and clinical implications. Curr Neuropharmacol. 2010;8(3):254-67.
  • 7. Loscher W. Critical review of current animal models of seizures and epilepsy used in the discovery and development of new antiepileptic drugs. Seizure. 2011;20(5):359-68.
  • 8. Samokhina E, Samokhin A. Neuropathological profile of the pentylenetetrazol (PTZ) kindling model. Int J Neurosci. 2018;128(11):1086-96.
  • 9. Li B, Wang L, Sun Z, Zhou Y, Shao D, Zhao J, et al. The anticonvulsant effects of SR 57227 on pentylenetetrazole-induced seizure in mice. PLoS One. 2014;9(4):e93158.
  • 10. Farrugia G, Szurszewski JH. Carbon monoxide, hydrogen sulfide, and nitric oxide as signaling molecules in the gastrointestinal tract. Gastroenterology. 2014;147(2):303-13.
  • 11. Shefa U, Yeo SG, Kim MS, Song IO, Jung J, Jeong NY, et al. Role of Gasotransmitters in Oxidative Stresses, Neuroinflammation, and Neuronal Repair. Biomed Res Int. 2017;2017:1689341.
  • 12. Shefa U, Kim D, Kim MS, Jeong NY, Jung J. Roles of Gasotransmitters in Synaptic Plasticity and Neuropsychiatric Conditions. Neural Plast. 2018;2018:1824713.
  • 13. Yang ZX, Qin J, Du JB, Chang XZ, Han Y. [Effect of endogenous heme oxygenase-carbon monoxide on brain damage induced by recurrent febrile seizures]. Zhonghua Er Ke Za Zhi. 2005;43(4):252-5.
  • 14. Luo Y, Wu PF, Zhou J, Xiao W, He JG, Guan XL, et al. Aggravation of seizure-like events by hydrogen sulfide: involvement of multiple targets that control neuronal excitability. CNS Neurosci Ther. 2014;20(5):411-9.
  • 15. Herberg LJ, Grottick A, Rose IC. Nitric oxide synthesis, epileptic seizures and kindling. Psychopharmacology (Berl). 1995;119(1):115-23.
  • 16. Osonoe K, Mori N, Suzuki K, Osonoe M. Antiepileptic effects of inhibitors of nitric oxide synthase examined in pentylenetetrazol-induced seizures in rats. Brain Res. 1994;663(2):338-40.
  • 17. Buisson A, Lakhmeche N, Verrecchia C, Plotkine M, Boulu RG. Nitric oxide: an endogenous anticonvulsant substance. Neuroreport. 1993;4(4):444-6. 18. de Oliveira CC, de Oliveira CV, Grigoletto J, Ribeiro LR, Funck VR, Grauncke AC, et al. Anticonvulsant activity of beta-caryophyllene against pentylenetetrazol-induced seizures. Epilepsy Behav. 2016;56:26-31.
  • 19. Kang X, Shen X, Xia Y. Electroacupuncture-induced attenuation of experimental epilepsy: a comparative evaluation of acupoints and stimulation parameters. Evid Based Complement Alternat Med. 2013;2013:149612.
  • 20. Dhir A, Rogawski MA. Determination of minimal steady-state plasma level of diazepam causing seizure threshold elevation in rats. Epilepsia. 2018;59(5):935-44.
  • 21. Polhemus DJ, Lefer DJ. Emergence of hydrogen sulfide as an endogenous gaseous signaling molecule in cardiovascular disease. Circ Res. 2014;114(4):730-7.
  • 22. Panthi S, Manandhar S, Gautam K. Hydrogen sulfide, nitric oxide, and neurodegenerative disorders. Transl Neurodegener. 2018;7:3.
  • 23. Leffler CW, Parfenova H, Jaggar JH, Wang R. Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation. J Appl Physiol (1985). 2006;100(3):1065-76.
  • 24. Hu LF, Lu M, Hon Wong PT, Bian JS. Hydrogen sulfide: neurophysiology and neuropathology. Antioxid Redox Signal. 2011;15(2):405-19.
  • 25. Kimura H, Nagai Y, Umemura K, Kimura Y. Physiological roles of hydrogen sulfide: synaptic modulation, neuroprotection, and smooth muscle relaxation. Antioxid Redox Signal. 2005;7(5-6):795-803.
  • 26. Abe K, Kimura H. The possible role of hydrogen sulfide as an endogenous neuromodulator. J Neurosci. 1996;16(3):1066-71.
  • 27. Tang G, Wu L, Wang R. Interaction of hydrogen sulfide with ion channels. Clin Exp Pharmacol Physiol. 2010;37(7):753-63.
  • 28. Otterbein LE. Carbon monoxide: innovative anti-inflammatory properties of an age-old gas molecule. Antioxid Redox Signal. 2002;4(2):309-19.
  • 29. Otterbein LE, Soares MP, Yamashita K, Bach FH. Heme oxygenase-1: unleashing the protective properties of heme. Trends Immunol. 2003;24(8):449-55.
  • 30. Fredenburgh LE, Merz AA, Cheng S. Haeme oxygenase signalling pathway: implications for cardiovascular disease. Eur Heart J. 2015;36(24):1512-8.
  • 31. Joshi HP, Kim SB, Kim S, Kumar H, Jo MJ, Choi H, et al. Nanocarrier-mediated Delivery of CORM-2 Enhances Anti-allodynic and Anti-hyperalgesic Effects of CORM-2. Mol Neurobiol. 2019;56(8):5539-54.
  • 32. Al-Huseini LM, Aw Yeang HX, Hamdam JM, Sethu S, Alhumeed N, Wong W, et al. Heme oxygenase-1 regulates dendritic cell function through modulation of p38 MAPK-CREB/ATF1 signaling. J Biol Chem. 2014;289(23):16442-51.
  • 33. Crespy D, Landfester K, Schubert US, Schiller A. Potential photoactivated metallopharmaceuticals: from active molecules to supported drugs. Chem Commun (Camb). 2010;46(36):6651-62.
  • 34. Queiroga CS, Vercelli A, Vieira HL. Carbon monoxide and the CNS: challenges and achievements. Br J Pharmacol. 2015;172(6):1533-45.
  • 35. Zimmermann A, Leffler CW, Tcheranova D, Fedinec AL, Parfenova H. Cerebroprotective effects of the CO-releasing molecule CORM-A1 against seizure-induced neonatal vascular injury. Am J Physiol Heart Circ Physiol. 2007;293(4):H2501-7.
  • 36. Banach M, Piskorska B, Czuczwar SJ, Borowicz KK. Nitric oxide, epileptic seizures, and action of antiepileptic drugs. CNS Neurol Disord Drug Targets. 2011;10(7):808-19.
  • 37. Jelenkovic A, Jovanovic M, Ninkovic M, Maksimovic M, Bokonjic D, Boskovic B. Nitric oxide (NO) and convulsions induced by pentylenetetrazol. Ann N Y Acad Sci. 2002;962:296-305.
Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Ezgi Eroğlu 0000-0002-9878-3431

Çiğdem Çengelli Ünel Bu kişi benim 0000-0001-7680-0141

Nuşin Harmancı Bu kişi benim 0000-0002-5777-2162

Zuhal Kaltuş Bu kişi benim 0000-0003-2281-1712

Cansu Kılıç Bu kişi benim 0000-0003-1100-9417

Hatice Doğan 0000-0002-9616-8910

Rabiye Altınbaş 0000-0003-2535-0480

Kevser Erol 0000-0002-8808-6616

Proje Numarası 2019/11D05
Yayımlanma Tarihi 16 Ekim 2020
Gönderilme Tarihi 29 Ocak 2020
Kabul Tarihi 13 Mart 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 15 Sayı: 3

Kaynak Göster

AMA Eroğlu E, Çengelli Ünel Ç, Harmancı N, Kaltuş Z, Kılıç C, Doğan H, Altınbaş R, Erol K. The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats. KSÜ Tıp Fak Der. Ekim 2020;15(3):1-6. doi:10.17517/ksutfd.681282