Determination of Total Phenolic Contents and Antioxidant Activities of Different Extracts Obtained from Morus alba L. (White Mulberry) Leaf
Öz
Objective: The plant known as white mulberry, or Morus alba L., has several uses and is employed in traditional medicine in many different cultures. The objectives of this study are to determine the total phenolic component and antioxidant levels of the methanol and ethanol extracts of leaves of Morus alba L. (White mulberry).
Methods: Using the techniques of DPPH (1,1-diphenyl-2-picrylhydrazyl), FRAP (Iron ion that decreases antioxidant power), and CUPRAC (Copper ion that reduces antioxidant capacity), the antioxidant activities of methanol and ethanol extracts of Morus alba L. leaves were examined. Additionally, the Folin-Ciocalteu Reagent (FCR) technique was used to evaluate the total phenolic content of the ethanol and methanol extracts. To determine the antioxidant capacity of extracts, reference samples were used to prepare various concentrations ranging from 1 to 100 μg/mL. The extract's equivalent antioxidant capacity was determined using sample concentrations of 125, 250, and 500 μg/mL.
Results: The methanol and ethanol extracts of the leaves of the Morus alba L. (White mulberry) plant were found to have the greatest level of phenolic compounds at the 500 μg/mL concentration of extracts. It was determined that the Trolox Eq value of methanol extract was higher in the DPPH, FRAP and FCR methods, and the Trolox Eq value of the ethanol extract was higher in the CUPRAC method.
Conclusion: The possible folk medicinal application of Morus alba L. is supported by this study. Determining the precise mechanisms of action of the extracts, the best extraction technique, the ideal dosage, and any possible adverse effects is needed.
Anahtar Kelimeler
CUPRAC DPPH ethanol extract FRAP methanol extract Morus alba L.
Kaynakça
- 1. Sharifi-Rad M, Anil Kumar NV, Zucca P, et al. Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Frontiers in physiology, 2020, 11: 694. [CrossRef]
- 2. Mititelu RR, Pădureanu R, Băcănoiu M, et al. Inflammatory and oxidative stress markers—mirror tools in rheumatoid arthritis. Biomedicines, 2020, 8: 125. [CrossRef]
- 3. Popović-Djordjević J, Quispe C, Giordo R, et al. Natural products and synthetic analogues against HIV: A perspective to develop new potential anti-HIV drugs. European journal of medicinal chemistry, 2022, 233: 114217. [CrossRef]
- 4. Chen C, Mohamad Razali UH, Saikim FH, Mahyudin A, Mohd Noor NQI. Morus alba L. plant: Bioactive compounds and potential as a functional food ingredient. Foods, 2021, 10: 689. [CrossRef]
- 5. Hao J, Gao Y, Xue J, et al. Phytochemicals, pharmacological effects and molecular mechanisms of mulberry. Foods, 2022, 11: 1170. [CrossRef]
- 6. Wen P, Hu T-G, Linhardt RJ, Liao S-T, Wu H, Zou Y-X. Mulberry: A review of bioactive compounds and advanced processing technology. Trends in Food Science & Technology, 2019, 83: 138-158. [CrossRef]
- 7. Chen C, Zhang B, Fu X, You L-J, Abbasi AM, Liu RH. The digestibility of mulberry fruit polysaccharides and its impact on lipolysis under simulated saliva, gastric and intestinal conditions. Food Hydrocolloids, 2016, 58: 171-178. [CrossRef]
- 8. Choi JW, Synytsya A, Capek P, Bleha R, Pohl R, Park YI. Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). Carbohydrate polymers, 2016, 146: 187-196. [CrossRef]
- 9. Lee JS, Synytsya A, Kim HB, et al. Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). International immunopharmacology, 2013, 17: 858-866. [CrossRef]
- 10. Li E, Long X, Liao S, Pang D, Li Q, Zou Y. Effect of mulberry galacto-oligosaccharide isolated from mulberry on glucose metabolism and gut microbiota in a type 2 diabetic mice. Journal of Functional Foods, 2021, 87: 104836. [CrossRef]
- 11. Lee Y, Hwang KT. Changes in physicochemical properties of mulberry fruits (Morus alba L.) during ripening. Scientia Horticulturae, 2017, 217: 189-196. [CrossRef]
Öz
Objective: The plant known as white mulberry, or Morus alba L., has several uses and is employed in traditional medicine in many different cultures. The objectives of this study are to determine the total phenolic component and antioxidant levels of the methanol and ethanol extracts of leaves of Morus alba L. (White mulberry).
Methods: Using the techniques of DPPH (1,1-diphenyl-2-picrylhydrazyl), FRAP (Iron ion that decreases antioxidant power), and CUPRAC (Copper ion that reduces antioxidant capacity), the antioxidant activities of methanol and ethanol extracts of Morus alba L. leaves were examined. Additionally, the Folin-Ciocalteu Reagent (FCR) technique was used to evaluate the total phenolic content of the ethanol and methanol extracts. To determine the antioxidant capacity of extracts, reference samples were used to prepare various concentrations ranging from 1 to 100 μg/mL. The extract's equivalent antioxidant capacity was determined using sample concentrations of 125, 250, and 500 μg/mL.
Results: The methanol and ethanol extracts of the leaves of the Morus alba L. (White mulberry) plant were found to have the greatest level of phenolic compounds at the 500 μg/mL concentration of extracts. It was determined that the Trolox Eq value of methanol extract was higher in the DPPH, FRAP and FCR methods, and the Trolox Eq value of the ethanol extract was higher in the CUPRAC method.
Conclusion: The possible folk medicinal application of Morus alba L. is supported by this study. Determining the precise mechanisms of action of the extracts, the best extraction technique, the ideal dosage, and any possible adverse effects is needed.
Anahtar Kelimeler
Etik Beyan
Ethical approval was not required as this study was conducted in vitro.
Kaynakça
- 1. Sharifi-Rad M, Anil Kumar NV, Zucca P, et al. Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Frontiers in physiology, 2020, 11: 694. [CrossRef]
- 2. Mititelu RR, Pădureanu R, Băcănoiu M, et al. Inflammatory and oxidative stress markers—mirror tools in rheumatoid arthritis. Biomedicines, 2020, 8: 125. [CrossRef]
- 3. Popović-Djordjević J, Quispe C, Giordo R, et al. Natural products and synthetic analogues against HIV: A perspective to develop new potential anti-HIV drugs. European journal of medicinal chemistry, 2022, 233: 114217. [CrossRef]
- 4. Chen C, Mohamad Razali UH, Saikim FH, Mahyudin A, Mohd Noor NQI. Morus alba L. plant: Bioactive compounds and potential as a functional food ingredient. Foods, 2021, 10: 689. [CrossRef]
- 5. Hao J, Gao Y, Xue J, et al. Phytochemicals, pharmacological effects and molecular mechanisms of mulberry. Foods, 2022, 11: 1170. [CrossRef]
- 6. Wen P, Hu T-G, Linhardt RJ, Liao S-T, Wu H, Zou Y-X. Mulberry: A review of bioactive compounds and advanced processing technology. Trends in Food Science & Technology, 2019, 83: 138-158. [CrossRef]
- 7. Chen C, Zhang B, Fu X, You L-J, Abbasi AM, Liu RH. The digestibility of mulberry fruit polysaccharides and its impact on lipolysis under simulated saliva, gastric and intestinal conditions. Food Hydrocolloids, 2016, 58: 171-178. [CrossRef]
- 8. Choi JW, Synytsya A, Capek P, Bleha R, Pohl R, Park YI. Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). Carbohydrate polymers, 2016, 146: 187-196. [CrossRef]
- 9. Lee JS, Synytsya A, Kim HB, et al. Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). International immunopharmacology, 2013, 17: 858-866. [CrossRef]
- 10. Li E, Long X, Liao S, Pang D, Li Q, Zou Y. Effect of mulberry galacto-oligosaccharide isolated from mulberry on glucose metabolism and gut microbiota in a type 2 diabetic mice. Journal of Functional Foods, 2021, 87: 104836. [CrossRef]
- 11. Lee Y, Hwang KT. Changes in physicochemical properties of mulberry fruits (Morus alba L.) during ripening. Scientia Horticulturae, 2017, 217: 189-196. [CrossRef]
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri (Diğer) |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 23 Nisan 2024 |
| Gönderilme Tarihi | 6 Aralık 2023 |
| Kabul Tarihi | 11 Ocak 2024 |
| Yayımlandığı Sayı | Yıl 2024 Cilt: 4 Sayı: 2 |
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