Abstract
Aim: Burns are one of the most difficult physical and psychological traumas that people face. Generally, protection
and prevention strategies from burns are practiced. The young and the elderly are most likely to be affected
tragically. The aim of the present study is to investigate the effect of Hypericum perforatum methanol extractcontaining gel on the healing of burn wounds.
Materials and Methods: Forty male rats of the Sprague-Dawley strain were divided into four groups after a 4x4 area
of their back was shaved, and an experimental burn was created with the direct contact of an aluminum metal stamp
heated in boiling water for 15 seconds. There was no implementation on or treatment of the control group (Group 1).
Burn wounds were irrigated with saline solution (Saline group, Group 2), and silver sulphadiazine 1% (Silverdin®)
cream (Group 3), Hypericum perforatum methanol extract-containing gel (Group 4), and a placebo gel (Group 5)
were applied topically 4 times a day after the contact burn. Histopathological analyses of the burned area were made
at 4, 8, and 24 hours.
Results: The topical use of Hypericum perforatum methanol extract-containing gel in the experimental contact burns,
histologically; resulted in the reduction of collagen discoloration, vascular damage and hair follicle and glandula
sebaceous damage while preserving total number of hair follicles, number of vessels and epidermal thickness
compared to Silver Sulphadiazine 1% (Silverdin®) cream treatment.
Conclusion: Positive effects of topical Hypericum perforatum gel were detected on experimental burns and its use
might have beneficial effects on acute burn wounds.
Keywords
References
- Şengezer M, Selmanpakoğlu N, Duman H, Çetin C. Epidemiological analysis of burn injuries in Gülhane Military Medical Academy Burn Center. Türk Plast Cer Derg 1995;3(1):74-7.
- Colasanti A, Kisslinger A, Liuzzi R, et al. Hypericin photosensitization of tumor and metastatic cell lines of human prostate. J Photochem Photobiol B 2000;54(2-3):103-7.
- Tang J, Colacino J.M, Larsen S.H. Virucidal activity of hypericin against enveloped and non-enveloped DNA and RNA viruses. Antiviral Res 1990;13(6):313-25.
- Reichling J, Weseler A, Saller R. A current review of the antimicrobial activity of Hypericum perforatum L. Pharmacopsychiatry 2001;34(1):126-9.
- Bayramoglu G, Bayramoglu A, Engur S, Senturk H, Ozturk N, Colak S. The hepatoprotective effects of Hypericum perforatum L. on hepatic ischemia/reperfusion injury in rats. Cytotechnology 2014;66(3):443-8.
- Otto M, Bach FW, Jensen TS, Sindrup SH. Health-related quality of life and its predictive role for analgesic effect in patients with painful polyneuropathy. Eur J Pain 2007;11(5):572-8.
- Pabuccuoğlu A, Konyalioğlu S, Bas M, Meral GE. The in vitro effects of Hypericum species on human leukocyte myeloperoxidase activity. J Ethnopharmacol 2003;57(1):59-92.
- Albert D, Zundorf I, Dingermann T, Muller WE, Steinhilber D, Werz O. Hyperforin is a dual inhibitor of cyclooxygenase-1 and 5-lipoxygenase. Biochem Pharmacol 2002;15(12):1767-75.
- Tedeschi E, Menegazzi M, Margotto D, Suzuki H, Forstermann U, Kleinert H. Antiinflammatory actions of St. John's Wort: Inhibition of human inducible nitric-oxide synthase expression by down-regulating signal transducer and activator of transcription-1alpha (STAT-1alpha) activation. J Pharmacol Exp Ther 2003;307(1):254-61.
- Miller AL. St. John's Wort (Hypericum perforatum) clinical effects on depression and other conditions. Altern Med Rev 1998;3(1):18-26.
- Rupal J, Kaushal J, Setty C, Mallikarjuna SC, Dipti P. Preparation and evaluation of topical gel of valdecoxib. Int J Pharmac Sci Drug Res 2010;2(1):51-4.
- Singer AJ, Berruti L, Thode HC Jr, McClain SA. Standardized burn model using a multiparametric histologic analysis of burn depth. Acad Emerg Med 2000;7(1):1-6.
- Li HL, Chen LP, Hu YH, et al. Crocodile oil enhances cutaneous burn wound healing and reduces scar formation in rats. Acad Emerg Med 2012;19(3):265-73.
- Demling RH. Burns and other thermal injuries. In: Lawrence W, Lang GM (eds). Current Surgical Diagnosis and Treatment. New York; Medical Books/McGraw-Hill; 2002:267-81.
- Taddonio TE, Thomson PD, Smith DJ, Prasad JK. A survey of wound monitoring and topical antimicrobial therapy practices in the treatment of burn injury. J Burn Care Rehabil 1990;11(5):423-7.
- Lee AR, Moon HK. Effect of topically applied silver sulfadiazine on fibroblast cell proliferation and biomechanical properties of the wound. Arch Pharm Res 2003;26(10):855-60.
- Stern HS. Silver sulphadiazine and the healing of partial thickness burns: A prospective clinical trial. Br J Plast Surg 1989;42(5):581-5.
- Akdemir O, Hede Y, Zhang F, Lineaweaver WC, Arslan Z, Songur E. Effects of taurine on reperfusion injury. J Plast Reconstr Aesthet Surg 2011;64(7):921-28.
- Burn incidence and treatment in the United States fact sheet, American Burn Association. Available at http://www.ameriburn.org. 2011.
- Meyerholz DK, Piester TL, Sokolich JC, Zamba GK, Light TD. Morphological parameters for assessment of burn severity in an acute burn injury rat model. Int J Exp Pathol 2009;90(1):26-33.
- Lavagna SM, Secci D, Chimenti P, Bonsignore L, Ottaviani A, Bizzarri B. Efficacy of Hypericum and Calendula oils in the epithelial reconstruction of surgical wounds in childbirth with caesarean section. II Farmaco 2001;56(5-7):451-3.
Abstract
Amaç: Yanık insanların karşılaştığı en zor fiziksel ve psikolojik travmalardan biridir. Genellikle yanıktan koruma ve
önleme stratejileri uygulanmaktadır. Yaşlılar ve gençler yanıktan en trajik olarak etkilenen popülasyondur. Bu
çalışmada, metanol ekstraksiyonu yöntemiyle elde edilen Hypericum perforatum (sarı kantaron) jelinin uzun süreli
olumlu etkilerinin araştırılması amaçlandı.
Gereç ve Yöntem: Kırk adet Sprague-Dawley cinsi erkek sıçanlar 100°C de 15 saniye kaynamış suda ısıtılmış
metalle dağlanarak yakılıp 5 gruba ayrıldı. Kontrol grubuna hiçbir işlem ve tedavi uygulanmadı (Grup 1). Salin
grubuna (Grup 2), yanık uygulaması yapılıp % 0, 9' luk NaCl solüsyonu ile irrigasyon yapıldı. Ticari bir ürün olan
Silverdin® kremi (Grup 3), metanol ekstrasyonu ile elde eldilmiş Hypericum perforatum jeli (Grup 4) ve plasebo jel
(Grup 5), aynı gün topikal olarak 4 kez uygulandı. Yanık alanı ve histopatolojik analizler 4., 8. ve 24. saatte biyopsi
alınıp değerlendirildi.
Bulgular: Metanol ekstrasyonu ile elde eldilmiş Hypericum perforatum jelinin deneysel yanık modeli sonrası topikal
uygulaması; kollajen diskolorizasyonunu, vasküler hasar, kıl folikülü ve sebasöz bezlerde meydana gelen hasarı
düşürürken ayrıca tüm damarların epidermal kalınlığının Silverdin®’e göre daha tedavi edici özellikte olduğu
gözlemlendi.
Sonuç: Deneysel yanıklar üzerinde Hypericum perforatum jelinin olumlu etkilerinin olduğu ve kullanımının akut
yanıklar üzerinde yararlı etkileri olabileceği görüldü.
Keywords
References
- Şengezer M, Selmanpakoğlu N, Duman H, Çetin C. Epidemiological analysis of burn injuries in Gülhane Military Medical Academy Burn Center. Türk Plast Cer Derg 1995;3(1):74-7.
- Colasanti A, Kisslinger A, Liuzzi R, et al. Hypericin photosensitization of tumor and metastatic cell lines of human prostate. J Photochem Photobiol B 2000;54(2-3):103-7.
- Tang J, Colacino J.M, Larsen S.H. Virucidal activity of hypericin against enveloped and non-enveloped DNA and RNA viruses. Antiviral Res 1990;13(6):313-25.
- Reichling J, Weseler A, Saller R. A current review of the antimicrobial activity of Hypericum perforatum L. Pharmacopsychiatry 2001;34(1):126-9.
- Bayramoglu G, Bayramoglu A, Engur S, Senturk H, Ozturk N, Colak S. The hepatoprotective effects of Hypericum perforatum L. on hepatic ischemia/reperfusion injury in rats. Cytotechnology 2014;66(3):443-8.
- Otto M, Bach FW, Jensen TS, Sindrup SH. Health-related quality of life and its predictive role for analgesic effect in patients with painful polyneuropathy. Eur J Pain 2007;11(5):572-8.
- Pabuccuoğlu A, Konyalioğlu S, Bas M, Meral GE. The in vitro effects of Hypericum species on human leukocyte myeloperoxidase activity. J Ethnopharmacol 2003;57(1):59-92.
- Albert D, Zundorf I, Dingermann T, Muller WE, Steinhilber D, Werz O. Hyperforin is a dual inhibitor of cyclooxygenase-1 and 5-lipoxygenase. Biochem Pharmacol 2002;15(12):1767-75.
- Tedeschi E, Menegazzi M, Margotto D, Suzuki H, Forstermann U, Kleinert H. Antiinflammatory actions of St. John's Wort: Inhibition of human inducible nitric-oxide synthase expression by down-regulating signal transducer and activator of transcription-1alpha (STAT-1alpha) activation. J Pharmacol Exp Ther 2003;307(1):254-61.
- Miller AL. St. John's Wort (Hypericum perforatum) clinical effects on depression and other conditions. Altern Med Rev 1998;3(1):18-26.
- Rupal J, Kaushal J, Setty C, Mallikarjuna SC, Dipti P. Preparation and evaluation of topical gel of valdecoxib. Int J Pharmac Sci Drug Res 2010;2(1):51-4.
- Singer AJ, Berruti L, Thode HC Jr, McClain SA. Standardized burn model using a multiparametric histologic analysis of burn depth. Acad Emerg Med 2000;7(1):1-6.
- Li HL, Chen LP, Hu YH, et al. Crocodile oil enhances cutaneous burn wound healing and reduces scar formation in rats. Acad Emerg Med 2012;19(3):265-73.
- Demling RH. Burns and other thermal injuries. In: Lawrence W, Lang GM (eds). Current Surgical Diagnosis and Treatment. New York; Medical Books/McGraw-Hill; 2002:267-81.
- Taddonio TE, Thomson PD, Smith DJ, Prasad JK. A survey of wound monitoring and topical antimicrobial therapy practices in the treatment of burn injury. J Burn Care Rehabil 1990;11(5):423-7.
- Lee AR, Moon HK. Effect of topically applied silver sulfadiazine on fibroblast cell proliferation and biomechanical properties of the wound. Arch Pharm Res 2003;26(10):855-60.
- Stern HS. Silver sulphadiazine and the healing of partial thickness burns: A prospective clinical trial. Br J Plast Surg 1989;42(5):581-5.
- Akdemir O, Hede Y, Zhang F, Lineaweaver WC, Arslan Z, Songur E. Effects of taurine on reperfusion injury. J Plast Reconstr Aesthet Surg 2011;64(7):921-28.
- Burn incidence and treatment in the United States fact sheet, American Burn Association. Available at http://www.ameriburn.org. 2011.
- Meyerholz DK, Piester TL, Sokolich JC, Zamba GK, Light TD. Morphological parameters for assessment of burn severity in an acute burn injury rat model. Int J Exp Pathol 2009;90(1):26-33.
- Lavagna SM, Secci D, Chimenti P, Bonsignore L, Ottaviani A, Bizzarri B. Efficacy of Hypericum and Calendula oils in the epithelial reconstruction of surgical wounds in childbirth with caesarean section. II Farmaco 2001;56(5-7):451-3.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | June 28, 2019 |
| Submission Date | March 8, 2018 |
| Published in Issue | Year 2019 |
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