Öz
Aim: The purpose of this study was to investigate the effects of varying doses of Cefazolin on in vitro fibroblast culture.
Material and Methods: In this study, the 3T3 mouse embryonic fibroblast cell line, which is widely used in in-vitro healing models, was used. Different doses of cefazolin, determined as 0 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml, 500 μg/ml and 750 μg/ml, were applied in-vitro on 3T3 mouse fibroblast cells. Experiments were planned as 24 and 48 hours. At the end of these periods, each group was evaluated with CCK-8 cell proliferation, migration and oxidative stress tests.
Results: The IC50 values of the groups were found to be in the range of 500- 750 μg/ml at the end of the 24th and 48th hours, respectively. On the other hand, it was observed that cefazolin administered in the dose range of 50-500 μg/ml did not have a negative effect on cell proliferation. While it was determined that the migration rate decreased depending on dose and time, no significant difference was found in terms of oxidative stress in the experimental groups in which cefazolin was administered at different dose intervals.
Conclusion: Cefazolin, administered pre- and postoperatively within therapeutic limits, did not cause any adverse effects on 3T3 mouse fibroblast cells in vitro.
Anahtar Kelimeler
Kaynakça
- Ponce B, Raines BT, Reed RD, Vick C, Richman J, Hawn M. Surgical Site Infection After Arthroplasty: Comparative Effectiveness of Prophylactic Antibiotics: Do Surgical Care Improvement Project Guidelines Need to Be Updated? J Bone Joint Surg Am. 2014 Jun 18; 96 (12): 970-7.
- Ramo BA, Roberts DW, Tuason D et al. Surgical site infections after posterior spinal fusion for neuromuscular scoliosis: a thirty-year experience at a single institution. J Bone Joint Surg Am. 2014 Dec 17; 96 (24): 2038-48.
- Yazdi IK, Murphy MB, Loo C et al. Cefazolin-loaded mesoporous silicon microparticles show sustained bactericidal effect against Staphylococcus aureus. J Tissue Eng. 2014 May 19; 5: 2041731414536573.
- Williams DN, Gustilo RB, Beverly R, Kind AC. Bone and serum concentrations of five cephalosporin drugs. Relevance to prophylaxis and treatment in orthopedic surgery. Clin Orthop Relat Res. 1983 Oct; (179): 253-65.
- Courtney PM, Melnic CM, Zimmer Z, Anari J, Lee GC. Addition of Vancomycin to Cefazolin Prophylaxis Is Associated With Acute Kidney Injury After Primary Joint Arthroplasty. Clin Orthop Relat Res. 2015 Jul; 473 (7): 2197-203.
- Bicanic G, Crnogaca K, Barbaric K, Delimar D. Cefazolin should be administered maximum 30 min before incision in total knee arthroplasty when tourniquet is used. Med Hypotheses. 2014 Jun;82 (6): 766-8.
- Southwell-Keely JP, Russo RR, March L, Cumming R, Cameron I, Brnabic AJ. Antibiotic prophylaxis in hip fracture surgery: a metaanalysis. Clin Orthop Relat Res. 2004 Feb; (419): 179-84.
- Pilge H, Fröbel J, Lensing-Höhn S, Zilkens C, Krauspe R. Cefazolin Irreversibly Inhibits Proliferation and Migration of Human Mesenchymal Stromal Cells. Biomed Res Int. 2016; 2016: 2042687.
- Salzmann GM, Naal FD, von Knoch F et al. Effects of cefuroxime on human osteoblasts in vitro. J Biomed Mater Res A. 2007 Aug; 82 (2): 462-8
- Naal FD, Salzmann GM, von Knoch F et al. The effects of clindamycin on human osteoblasts in vitro. Arch Orthop Trauma Surg. 2008 Mar; 128 (3): 317-23.
- Zhang Z, Wang X, Luo F et al. Effects of rifampicin on osteogenic differentiation and proliferation of human mesenchymal stem cells in the bone marrow. Genet Mol Res. 2014 Aug 25; 13 (3): 6398-410.
- Wang JH, Guo Q, Li B. Tendon biomechanics and mechanobiology--a minireview of basic concepts and recent advancements. J Hand Ther. 2012 Apr-Jun; 25 (2): 133-40; quiz 141.
- Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008 May 15; 453 (7193): 314-21.
- desJardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update. Regen Med. 2018 Jul 1; 13 (5): 491-5.
- Kaleci B, Koyuturk M. Efficacy of resveratrol in the wound healing process by reducing oxidative stress and promoting fibroblast cell proliferation and migration. Dermatol Ther. 2020 Nov; 33 (6): e14357. Pulido L, Ghanem E, Joshi A, Purtill JJ, Parvizi J. Periprosthetic joint infection: the incidence, timing, and predisposing factors. Clin Orthop Relat Res. 2008 Jul; 466 (7): 1710-5.
- Haasters F, Docheva D, Gassner C et al. Mesenchymal stem cells from osteoporotic patients reveal reduced migration and invasion upon stimulation with BMP-2 or BMP-7. Biochem Biophys Res Commun. 2014 Sep 12; 452 (1): 118-23.
- Siengdee P, Pradit W, Euppayo T, Chomdej S, Nganvongpanit K. Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture. J Vet Pharmacol Ther. 2017 Dec; 40 (6): 604-17.
Öz
Amaç: Sefazolin’in değişen dozlarının in vitro fibroblast kültürü üzerine olan etkilerinin araştırılması amaçlandı.
Gereç ve Yöntem: Bu çalışmada, in vitro iyileşme modellemelerinde yaygın olarak kullanılan 3T3 fare embriyonik fibroblast hücre soyu kullanıldı ve sefazolinin 0 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml, 500 μg/ml ve 750 μg/ml olarak belirlenen farklı dozları in-vitro olarak 3T3 fare fibroblast hücreleri üzerine uygulandı. Deneyler 24 ve 48 saat olarak planlandı. Bu sürelerin sonunda her bir grup, CCK-8 hücre proliferasyon, migrasyon ve oksidatif stres testleri ile değerlendirildi.
Bulgular: Gruplara ait IC50 değerleri 24 ve 48. saatler sonunda sırası ile 500- 750 μg/ml doz aralığında olarak bulundu. Buna karşın 50-500 μg/ml doz aralığında uygulanan sefazolinin ise hücre proliferasyonu üzerine olumsuz bir etki göstermediği görüldü. Migrasyon hızının doz ve zaman bağımlı olarak azaldığı tespit edilirken, buna karşın sefazolinin farklı doz aralıklarında uygulandığı deney gruplarında oksidatif stres açısından anlamlı bir fark tespit edilmedi.
Sonuç: Terapötik sınırlarda pre ve postoperatif olarak uygulanan sefazolin in vitro koşullarda 3T3 fare fibroblast hücreleri üzerinde olumsuz bir etki oluşturmamıştır.
Anahtar Kelimeler
Kaynakça
- Ponce B, Raines BT, Reed RD, Vick C, Richman J, Hawn M. Surgical Site Infection After Arthroplasty: Comparative Effectiveness of Prophylactic Antibiotics: Do Surgical Care Improvement Project Guidelines Need to Be Updated? J Bone Joint Surg Am. 2014 Jun 18; 96 (12): 970-7.
- Ramo BA, Roberts DW, Tuason D et al. Surgical site infections after posterior spinal fusion for neuromuscular scoliosis: a thirty-year experience at a single institution. J Bone Joint Surg Am. 2014 Dec 17; 96 (24): 2038-48.
- Yazdi IK, Murphy MB, Loo C et al. Cefazolin-loaded mesoporous silicon microparticles show sustained bactericidal effect against Staphylococcus aureus. J Tissue Eng. 2014 May 19; 5: 2041731414536573.
- Williams DN, Gustilo RB, Beverly R, Kind AC. Bone and serum concentrations of five cephalosporin drugs. Relevance to prophylaxis and treatment in orthopedic surgery. Clin Orthop Relat Res. 1983 Oct; (179): 253-65.
- Courtney PM, Melnic CM, Zimmer Z, Anari J, Lee GC. Addition of Vancomycin to Cefazolin Prophylaxis Is Associated With Acute Kidney Injury After Primary Joint Arthroplasty. Clin Orthop Relat Res. 2015 Jul; 473 (7): 2197-203.
- Bicanic G, Crnogaca K, Barbaric K, Delimar D. Cefazolin should be administered maximum 30 min before incision in total knee arthroplasty when tourniquet is used. Med Hypotheses. 2014 Jun;82 (6): 766-8.
- Southwell-Keely JP, Russo RR, March L, Cumming R, Cameron I, Brnabic AJ. Antibiotic prophylaxis in hip fracture surgery: a metaanalysis. Clin Orthop Relat Res. 2004 Feb; (419): 179-84.
- Pilge H, Fröbel J, Lensing-Höhn S, Zilkens C, Krauspe R. Cefazolin Irreversibly Inhibits Proliferation and Migration of Human Mesenchymal Stromal Cells. Biomed Res Int. 2016; 2016: 2042687.
- Salzmann GM, Naal FD, von Knoch F et al. Effects of cefuroxime on human osteoblasts in vitro. J Biomed Mater Res A. 2007 Aug; 82 (2): 462-8
- Naal FD, Salzmann GM, von Knoch F et al. The effects of clindamycin on human osteoblasts in vitro. Arch Orthop Trauma Surg. 2008 Mar; 128 (3): 317-23.
- Zhang Z, Wang X, Luo F et al. Effects of rifampicin on osteogenic differentiation and proliferation of human mesenchymal stem cells in the bone marrow. Genet Mol Res. 2014 Aug 25; 13 (3): 6398-410.
- Wang JH, Guo Q, Li B. Tendon biomechanics and mechanobiology--a minireview of basic concepts and recent advancements. J Hand Ther. 2012 Apr-Jun; 25 (2): 133-40; quiz 141.
- Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008 May 15; 453 (7193): 314-21.
- desJardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update. Regen Med. 2018 Jul 1; 13 (5): 491-5.
- Kaleci B, Koyuturk M. Efficacy of resveratrol in the wound healing process by reducing oxidative stress and promoting fibroblast cell proliferation and migration. Dermatol Ther. 2020 Nov; 33 (6): e14357. Pulido L, Ghanem E, Joshi A, Purtill JJ, Parvizi J. Periprosthetic joint infection: the incidence, timing, and predisposing factors. Clin Orthop Relat Res. 2008 Jul; 466 (7): 1710-5.
- Haasters F, Docheva D, Gassner C et al. Mesenchymal stem cells from osteoporotic patients reveal reduced migration and invasion upon stimulation with BMP-2 or BMP-7. Biochem Biophys Res Commun. 2014 Sep 12; 452 (1): 118-23.
- Siengdee P, Pradit W, Euppayo T, Chomdej S, Nganvongpanit K. Comparison of the effects of cefazolin and ceftriaxone on canine chondrocyte culture. J Vet Pharmacol Ther. 2017 Dec; 40 (6): 604-17.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Mart 2022 |
| Gönderilme Tarihi | 19 Aralık 2021 |
| Yayımlandığı Sayı | Yıl 2022 |