Hiperbarik oksijen tedavisinin MCF-7 meme kanseri hücrelerinin morfolojik özellikleri ve sağkalımı üzerindeki etkileri
Öz
Amaç: Bu çalışma, MCF-7 meme kanseri hücre hattına farklı basınçlar altında uygulanan hiperbarik oksijen tedavisinin hücre morfolojisi ve hücre sağkalımı üzerindeki etkilerini belirlemeyi amaçlamaktadır.
Gereç ve Yöntem: MCF-7 meme kanser hücrelerine 1,5, 2 ve 2,5 atmosfer basınç altında 2 saat boyunca %100 oksijen uygulanarak deney grupları oluşturuldu. Kontrol grubuna ise herhangi bir tedavi uygulanmadı. Deney sonunda; hücre sağkalımları CCK-8 analizi hücre şekilleri cristal viyole boyama ve hücre yüzey morfolojileri taramalı elektron mikroskobu ile belirlendi.
Bulgular: Hücre canlılığı, kontrol grubuna kıyasla 1,5, 2 ve 2,5 atmosfer basıncında önemli ölçüde azaldı (p < 0.005). Basınç arttıkça hücre yüzey alanının azaldığı, nükleer yoğunlaşmanın arttığı ve hücre sınırlarının düzensizleştiği görüldü. İki ve 2,5 atmosfer basınçta, hücre zarının kabarcık şekilli protrüzyonu ve orasyonunda artış tespit edildi.
Sonuç: Hiperbarik oksijen tedavisi artan basınçla orantılı olarak MCF-7 meme kanseri hücrelerinin canlılığını ciddi şekilde azaltır. Hücre şeklini ve yüzey morfolojilerini değiştirir. MCF-7 meme kanseri hücrelerinde apoptozu tetikleyebilir. Daha ileri çalışmalara ihtiyaç duyulmasına rağmen, çalışmamız meme kanseri tedavisinde hiperbarik oksijen tedavisinin kullanım potansiyelini desteklemektedir.
Anahtar Kelimeler
Meme kanseri MCF-7 Hiperbarik oksijen tedavisi Taramalı elektron mikroskobu CCK-8
Kaynakça
- Ferlay J, Colombet M, Soerjomataram I, et al. Cancer statistics for the year 2020: An overview. Int J Cancer 2021;Apr 5.
- Trayes KP, Cokenakes SEH. Breast Cancer Treatment. Am Fam Physician 2021;Aug 1;104(2):171-8.
- Leach RM, Rees PJ, Wilmshurst P. Hyperbaric oxygen therapy. BMJ 1998;Oct 24;317(7166):1140-3.
- Ortega MA, Fraile-Martinez O, García-Montero C, et al. A General Overview on the Hyperbaric Oxygen Therapy: Applications, Mechanisms and Translational Opportunities Medicina (Kaunas). 2021;Aug 24;57(9):864.
- Lyons SM, Alizadeh E, Mannheimer J, et al. Changes in cell shape are correlated with metastatic potential in murine and human osteosarcomas. Biol Open 2016;Feb 12;5(3):289-99.
- Ishikura N, Sugimoto M, Yorozu K, Kurasawa M, Wakita D, Kondoh O. Effect of Bevacizumab on a Human Breast Cancer Model that Exhibited Palbociclib-resistance by RB Knockout. Cancer Diagn Progn 2022;Sep 3;2(5):533-41.
- Tang W, Peng W, Zhang H, Zhang Y, Li B, Duan C. Period 3, a tumor suppressor in non-small cell lung cancer, is silenced by hypermethylation. Int J Clin Exp Pathol 2018;Jan 1;11(1):120-8.
- Geserick P, Hupe M, Moulin M, et al. Cellular IAPs inhibit a cryptic CD95-induced cell death by limiting RIP1 kinase recruitment. J Cell Biol 2009;Dec 28;187(7):1037-54.
- Muz B, de la Puente P, Azab F, Azab AK. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia (Auckl) 2015;Dec 11;3:83-92.
- Tutzauer J, Sjöström M, Holmberg E, et al. Breast cancer hypoxia in relation to prognosis and benefit from radiotherapy after breast-conserving surgery in a large, randomised trial with long-term follow-up. Br J Cancer 2022;May;126(8):1145-56.
- Xue T, Ding JS, Li B, Cao DM, Chen G. A narrative review of adjuvant therapy for glioma: hyperbaric oxygen therapy. Med Gas Res 2021;Oct-Dec;11(4):155-7.
- Granowitz EV, Tonomura N, Benson RM, et al. Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation. Anticancer Res 2005;Nov-Dec;25(6B):3833-42.
- Chen YC, Chen SY, Ho PS, et al. Apoptosis of T-leukemia and B-myeloma cancer cells induced by hyperbaric oxygen increased phosphorylation of p38 MAPK. Leuk Res 2007;Jun;31(6):805-15.
- G Bosco, L Guizzon, Z Yang, E Camporesi, Effect of hyperbaric oxygenation and gemcitabine on apoptosis of pancreatic ductal tumor cells in vitro. Anticancer Res. 2013 Nov;33(11):4827-32.
- Ganguly BJ, Tonomura N, Benson RM, Osborne BA, Granowitz EV. Hyperbaric oxygen enhances apoptosis in hematopoietic cells. Apoptosis 2002; Dec;7(6):499-510.
- Weber SU, Koch A, Kankeleit J, et al. Hyperbaric oxygen induces apoptosis via a mitochondrial mechanism. Apoptosis 2009;14, 97-107.
- Takano KJ, Takano T, Yamanouchi Y, Satou T. Pressure-induced apoptosis in human lymphoblasts. Exp Cell Res 1997;Aug 25;235(1):155-60.
- Chen SY, Tsuneyama K, Yen MH, Lee JT, Chen JL, Huang SM. Hyperbaric oxygen suppressed tumor progression through the improvement of tumor hypoxia and induction of tumor apoptosis in A549-cell-transferred lung cancer. Sci Rep 2021;Jun 8;11(1):12033.
- Tang H, Zhang ZY, Ge JP, Zhou WQ, Gao JP. Effects of hyperbaric oxygen on tumor growth in the mouse model of LNCaP prostate cancer cell line. Zhonghua Nan Ke Xue 2009;Aug;15(8):713-6. Chinese
- Qi Y, Ruan J, Wang M, et al. Effects of hyperbaric oxygen treatment on gastric cancer cell line SGC7901. Biomed Rep 2017;Apr;6(4):475-9.
- Flores Romero H, Ros U, Garcia Saez AJ. Pore formation in regulated cell death. EMBO J 2020;Dec 1;39(23):e105753.
- Aguilar AA, Ho MC, Chang E, et al. Permeabilizing Cell Membranes with Electric Fields. Cancers (Basel) 2021;May 10;13(9):2283.
- He S, Singh D, Helfield B. An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery. Pharmaceutics 2022;Apr 18;14(4):886.
- Khajah MA, Luqmani YA. Involvement of Membrane Blebbing in Immunological Disorders and Cancer. Med Princ Pract 2016;25 Suppl 2(Suppl 2):18-27.
- Aoki K, Satoi S, Harada S, Uchida S, Iwasa Y, Ikenouchi J. Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb. Mol Biol Cell 2020;Apr 1;31(8):833-44.
Effects of hyperbaric oxygen therapy on the morphological characteristics and survival of MCF-7 breast cancer cells
Öz
Aim: This study aims to determine the effects of hyperbaric oxygen therapy at different pressure values on cell morphology and cell survival in the MCF-7 breast cancer cell line.
Materials and Methods: The experimental groups were formed by applying 100% oxygen to MCF-7 breast cancer cells at 1.5, 2, and 2.5 atmospheres for 2 hours. The control group did not receive treatment. At the end of the experiment, cell survival was investigated by CCK-8 analysis, cell shapes were determined by cresyl violet staining, and cell surface morphologies were determined by scanning electron microscope.
Results: Cell viability was significantly reduced at atmospheric pressure of 1.5, 2, and 2.5 compared to the control group (p < 0.005). As pressure increased, the surface area of the cell decreased, nuclear condensation increased, and the cell borders became irregular. Cell membrane bleb and cell membrane porosity increased at 2 and 2.5 atmospheres.
Conclusion: Hyperbaric oxygen therapy severely reduces the viability of MCF-7 breast cancer cells under increased pressure. It can induce apoptosis and change the shape and surface morphology of MCF-7 breast cancer cells. Although further studies are needed, our study supports the potential use of hyperbaric oxygen therapy in the treatment of breast cancer.
Anahtar Kelimeler
Breast cancer MCF-7 Hyperbaric oxygen therapy Scanning electron microscope CCK-8
Kaynakça
- Ferlay J, Colombet M, Soerjomataram I, et al. Cancer statistics for the year 2020: An overview. Int J Cancer 2021;Apr 5.
- Trayes KP, Cokenakes SEH. Breast Cancer Treatment. Am Fam Physician 2021;Aug 1;104(2):171-8.
- Leach RM, Rees PJ, Wilmshurst P. Hyperbaric oxygen therapy. BMJ 1998;Oct 24;317(7166):1140-3.
- Ortega MA, Fraile-Martinez O, García-Montero C, et al. A General Overview on the Hyperbaric Oxygen Therapy: Applications, Mechanisms and Translational Opportunities Medicina (Kaunas). 2021;Aug 24;57(9):864.
- Lyons SM, Alizadeh E, Mannheimer J, et al. Changes in cell shape are correlated with metastatic potential in murine and human osteosarcomas. Biol Open 2016;Feb 12;5(3):289-99.
- Ishikura N, Sugimoto M, Yorozu K, Kurasawa M, Wakita D, Kondoh O. Effect of Bevacizumab on a Human Breast Cancer Model that Exhibited Palbociclib-resistance by RB Knockout. Cancer Diagn Progn 2022;Sep 3;2(5):533-41.
- Tang W, Peng W, Zhang H, Zhang Y, Li B, Duan C. Period 3, a tumor suppressor in non-small cell lung cancer, is silenced by hypermethylation. Int J Clin Exp Pathol 2018;Jan 1;11(1):120-8.
- Geserick P, Hupe M, Moulin M, et al. Cellular IAPs inhibit a cryptic CD95-induced cell death by limiting RIP1 kinase recruitment. J Cell Biol 2009;Dec 28;187(7):1037-54.
- Muz B, de la Puente P, Azab F, Azab AK. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia (Auckl) 2015;Dec 11;3:83-92.
- Tutzauer J, Sjöström M, Holmberg E, et al. Breast cancer hypoxia in relation to prognosis and benefit from radiotherapy after breast-conserving surgery in a large, randomised trial with long-term follow-up. Br J Cancer 2022;May;126(8):1145-56.
- Xue T, Ding JS, Li B, Cao DM, Chen G. A narrative review of adjuvant therapy for glioma: hyperbaric oxygen therapy. Med Gas Res 2021;Oct-Dec;11(4):155-7.
- Granowitz EV, Tonomura N, Benson RM, et al. Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation. Anticancer Res 2005;Nov-Dec;25(6B):3833-42.
- Chen YC, Chen SY, Ho PS, et al. Apoptosis of T-leukemia and B-myeloma cancer cells induced by hyperbaric oxygen increased phosphorylation of p38 MAPK. Leuk Res 2007;Jun;31(6):805-15.
- G Bosco, L Guizzon, Z Yang, E Camporesi, Effect of hyperbaric oxygenation and gemcitabine on apoptosis of pancreatic ductal tumor cells in vitro. Anticancer Res. 2013 Nov;33(11):4827-32.
- Ganguly BJ, Tonomura N, Benson RM, Osborne BA, Granowitz EV. Hyperbaric oxygen enhances apoptosis in hematopoietic cells. Apoptosis 2002; Dec;7(6):499-510.
- Weber SU, Koch A, Kankeleit J, et al. Hyperbaric oxygen induces apoptosis via a mitochondrial mechanism. Apoptosis 2009;14, 97-107.
- Takano KJ, Takano T, Yamanouchi Y, Satou T. Pressure-induced apoptosis in human lymphoblasts. Exp Cell Res 1997;Aug 25;235(1):155-60.
- Chen SY, Tsuneyama K, Yen MH, Lee JT, Chen JL, Huang SM. Hyperbaric oxygen suppressed tumor progression through the improvement of tumor hypoxia and induction of tumor apoptosis in A549-cell-transferred lung cancer. Sci Rep 2021;Jun 8;11(1):12033.
- Tang H, Zhang ZY, Ge JP, Zhou WQ, Gao JP. Effects of hyperbaric oxygen on tumor growth in the mouse model of LNCaP prostate cancer cell line. Zhonghua Nan Ke Xue 2009;Aug;15(8):713-6. Chinese
- Qi Y, Ruan J, Wang M, et al. Effects of hyperbaric oxygen treatment on gastric cancer cell line SGC7901. Biomed Rep 2017;Apr;6(4):475-9.
- Flores Romero H, Ros U, Garcia Saez AJ. Pore formation in regulated cell death. EMBO J 2020;Dec 1;39(23):e105753.
- Aguilar AA, Ho MC, Chang E, et al. Permeabilizing Cell Membranes with Electric Fields. Cancers (Basel) 2021;May 10;13(9):2283.
- He S, Singh D, Helfield B. An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery. Pharmaceutics 2022;Apr 18;14(4):886.
- Khajah MA, Luqmani YA. Involvement of Membrane Blebbing in Immunological Disorders and Cancer. Med Princ Pract 2016;25 Suppl 2(Suppl 2):18-27.
- Aoki K, Satoi S, Harada S, Uchida S, Iwasa Y, Ikenouchi J. Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb. Mol Biol Cell 2020;Apr 1;31(8):833-44.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Mart 2023 |
| Gönderilme Tarihi | 5 Ekim 2022 |
| Yayımlandığı Sayı | Yıl 2023 |