Assessment of Resveratrol's effects comparatively with zinc in experimental rat testicular damage induced by Cyclophosphamide

Cansın Şirin; Canberk Tomruk; Elif Karadadaş; Burcu Denizlioğlu; Gülinnaz Ercan; Aslı Suner; Özlem Yılmaz

doi:10.19161/etd.1201643

EN TR

Assessment of Resveratrol's effects comparatively with zinc in experimental rat testicular damage induced by Cyclophosphamide

Abstract

Aim: To investigate protective effects of Resveratrol in combination or comparison with zinc in experimental testicular injury induced by Cyclophosphamide is studied for the first time in literature. Materials and Methods: Rats (n=63) were randomly divided into 9 groups. After 21 days of drug administration biochemical and histological analysis were performed. Daily water consumption, body weights and weight of testes were measured. Johnsen’s testicular scoring and sperm morphology were evaluated. Hematoxylin&Eosin, Periodic Acid-Schiff and Masson's Trichrome stainings and iNOS, eNOS and CD34 antibodies were applied histologically. To determine oxidative stress, MDA and CAT values were determined. Statistically, one-way ANOVA with post hoc Tukey HSD test for multiple comparisons was performed via IBM SPSS Version 25.0. Results: Cyclophosphamide caused an increase in testicular MDA levels due to elevated oxidant stress. Testicular MDA levels significantly decreased in zinc and Resveratrol groups which revealed protective effects related to Cyclophosphamide treatment, while no significant improvement was observed for control and saline groups. However, the most significant decrease was observed in MDA for Cyclophosphamide+zinc+Resveratrol group in comparison to Cyclophosphamide. Telocytes, which are lately defined novel cells, were detected in the interstitium encircling seminiferous tubules as a sheath immunohistochemically. Conclusion: Not only Resveratrol and zinc, but also their optimum administration separately protects testes in Cyclophosphamide treatment groups. Clinical adaptations of this in vivo model may lead to novel futuristic ideas in preventing infertility due to cancer chemotherapy.

Keywords

Siklofosfamid’in neden olduğu deneysel sıçan testis hasarında Resveratrol’ün etkilerinin çinko ile karşılaştırmalı olarak değerlendirilmesi

Öz

Amaç: Siklofosfamid'in neden olduğu deneysel testis hasarında Resveratrol’ün olası koruyucu etkilerini çinko ile kombine ya da kıyaslamalı olarak araştırılması amaçlanmıştır. Gereç ve Yöntem: Sıçanlar (n=63) rastgele 9 gruba ayrıldı. 21 günlük ilaç uygulaması sonunda; tüm gruplar biyokimyasal ve histolojik olarak incelendi. Günlük su tüketimleri ile deneklerin vücut ağırlıkları ve testis ağırlıkları ölçüldü. Johnsen'in testis skorlaması ve sperm morfolojileri değerlendirildi. Histolojik olarak Hematoksilen&Eozin, Periyodik Asit-Schiff ve Masson'un Trikrom boyaları ve iNOS, eNOS ve CD34 antikorları uygulandı. Doku oksidatif stres düzeyleri için MDA ve CAT değerleri biyokimyasal olarak saptandı. İstatistiksel değerlendirmelerde çoklu karşılaştırmalar için post hoc Tukey HSD testi ile tek yönlü ANOVA, IBM SPSS Sürüm 25.0 kullanıldı. Bulgular: Siklofosfamid, yüksek oksidan stres nedeniyle testis MDA düzeylerini arttırdı. Siklofosfamid tedavisine bağlı koruyucu etkiler ortaya çıkaran çinko ve Resveratrol gruplarında testis MDA seviyelerinde anlamlı düşüşe karşılık, kontrol ve salin gruplarında anlamlı bir iyileşme gözlenmedi. Ancak MDA'da en belirgin azalma Siklofosfamid’e göre Siklofosfamid+çinko+Resveratrol grubunda saptandı. Son zamanlarda yeni tanımlanan hücreler olan telositlerin interstisyumda kılıf şeklinde seminifer tübülleri çevreledikleri immünohistokimyasal olarak gösterildi. Sonuç: Siklofosfamid tedavisi altında testis dokularını korumada sadece Resveratrol ve çinkonun kombine kullanımı değil, optimum dozlarda ayrı ayrı uygulamaları da değerlidir. Bu in vivo modelin klinik uyarlamaları, kanser tedavisine bağlı potansiyel infertilitenin önlenmesinde yeni fikirlerin gelişmesine gelecekte öncülük edebilir.

Anahtar Kelimeler

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Cansın Şirin
Türkiye

Canberk Tomruk
Türkiye

Elif Karadadaş
Türkiye

Burcu Denizlioğlu
Türkiye

Gülinnaz Ercan
Türkiye

Aslı Suner
Türkiye

Özlem Yılmaz ^*
Türkiye

Yayımlanma Tarihi

15 Mart 2023

Gönderilme Tarihi

9 Kasım 2022

Kabul Tarihi

18 Aralık 2022

Yayımlandığı Sayı

Yıl 1970 Cilt: 62 Sayı: 1

DOI

https://doi.org/10.19161/etd.1201643

IZ

https://izlik.org/JA36GU45FY

Kaynak Göster

RIS / Bibtex

Vancouver

1.Cansın Şirin, Canberk Tomruk, Elif Karadadaş, Burcu Denizlioğlu, Gülinnaz Ercan, Aslı Suner, Özlem Yılmaz. Assessment of Resveratrol’s effects comparatively with zinc in experimental rat testicular damage induced by Cyclophosphamide. ETD. 01 Mart 2023;62(1):18-29. doi:10.19161/etd.1201643