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Cytotoxicity assay of Turkish rare endemic Helianthemum germanicopolitanum Bornm. plant extract on HT-29 cell line

Year 2024, Volume: 63 Issue: 3, 441 - 448, 09.09.2024

Kubilay Doğan Kılıç , Ayşenur Kayabaş Avşar , Cemre Özkanca , Dila Hatun Sal , Fatih Karabey , Zeynep Simge Yılmaz , Yiğit Uyanıkgil

https://doi.org/10.19161/etd.1441204

Abstract

Objective: The aim of this study was to investigate the cytotoxic effect of Helianthem germanicopolitanum Bornm., a rare endemic plant in Turkey, against colon cancer.
Materials and Methods: After the extraction of Helianthemum germanicopolitanum Bornm. plant, the phytochemical profile of the plant was analyzed by High Performance Liquid Chromatography (HPLC). Cytotoxicity analysis on HT-29 human colon cancer cell line was performed by WST 1 assay. The results were compared with other studies on similar plant species in the literature.
Results: H. germanicopolitanum plant contains various flavonoids and these flavonoids have cytotoxic effects on colorectal cancer cells. These cytotoxic effects provide anticarcinogenic effects by activating cell death pathways at certain concentrations. These findings provide an important insight into the therapeutic potential of the plant.
Conclusion: Cytotoxicity studies of flavonoids in extracts obtained from extraction procedures on colorectal cancer cell lines show that flavonoids offer anticarcinogenic effects by activating cell death pathways at certain concentrations.

Keywords

Helianthemum germanicopolitanum Colon Cancer HT-29 Cell Line Flavonoids Cytotoxicity

References

  • Jain A, Jain P, Soni P, Tiwari A, Tiwari SP. Design and characterization of silver nanoparticles of different species of Curcuma in the treatment of cancer using human colon cancer cell line (HT-29). J Gastrointest Cancer 2023;54(1):90-5.
  • TSCRS (Turkish Society of Colon and Rectal Surgery). (2023). https://www.tkrcd.org.tr/en/ [cited 21 November 2023].
  • WHO (World Health Organization). (2023). https://www.who.int/news-room/fact-sheets/detail/colorectal-cancer?gclid=Cj0KCQiApOyqBhDlARIsAGfnyMpXMoYCnlou8M2JjUr3FnbCTQPCrGRl7bDBWM1fV16Ob9fmpINYiocaArbpEALw_wcB [cited 21 November 2023].
  • Karahan F. Evaluation of trace element and heavy metal levels of some ethnobotanically important medicinal plants used as remedies in Southern Turkey in terms of human health risk. Biol Trace Elem Res 2023; 201(1): 493-513.
  • Bordoloi C, Kumar S, Barbhuiya AM, Kushari S, Kalita JM, Sahu BP, Laloo D. Herbal medicine used for wound healing by the tribes of the North Eastern States of India: A comprehensive review. J Herbal Med 2023;100697.
  • Kına E, Uysal İ, Mohammed FS, Doğan M, Sevindik M. In-vitro antioxidant and oxidant properties of Centaurea rigida. Turk J Agric Food Sci Technol 2021;9(10):1905-7.
  • Gong P, Long H, Guo Y, Wang Z, Yao W, Wang J, et al. Chinese herbal medicines: The modulator of nonalcoholic fatty liver disease targeting oxidative stress. J Ethnopharmacol 2024;318(Pt B):116927.
  • Kuruppu AI, Paranagama P, Goonasekara CL. Medicinal plants commonly used against cancer in traditional medicine formulae in Sri Lanka. Saudi Pharm J 2019;27(4):565-573.
  • Máthé Á, Turgut K. Introduction to Medicinal and Aromatic Plants in Türkiye. In: Medicinal and Aromatic Plants of Turkey. Cham: Springer International Publishing;2023:1-30.
  • Ağca A, Yılmaz Sarıaltın S, Sever Yılmaz B. Evaluation of free radical scavenging, anti-inflammatory and hypoglycemic activity of Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 2023;26(2):227-233.
  • Küpeli Akkol E, Kosar M, Baldemir A, Şeker Karatoprak G, Demirpolat E, Yerer Aycan MB, et al. The wound-healing potential of the endemic plant Helianthemum canum (L.) Baumg: Preclinical studies supported with phytochemical profiling. Chem Biodivers 2023;20(12):e202301529.
  • Laib I, Djahra AB. Phytochemical investigation of Helianthemum lippii l. aerial Dum. Cours part and evaluation for its antioxidant activities. International Journal of Secondary Metabolite 2022;9(2):229-237.
  • Djemam N, Lassed S, Gül F, Altun M, Monteiro M, Menezes-Pinto D, et al. Characterization of ethyl acetate and n-butanol extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon. J Ethnopharmacol 2020;252:112613.
  • Güner A, Aslan S, Ekim T, Vural M, Babaç MT (Editörler). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını; 2012:370.
  • Yeşilyurt EB, Erik S, Özmen E, Akaydın G. Comparative morphological, palynological and anatomical characteristics of Turkish rare endemics Helianthemum germanicopolitanum and Helianthemum antitauricum (Cistaceae). Plant Systematics and Evolution 2015;301:125-137.
  • İnan E, İpek G, İpek A. Çankırı’nın Endemik Tıbbi Bitkileri. Türk Bilimsel Derlemeler Dergisi 2012;(2):38-40
  • Öztürk M, Altay V. An overview of the endemic medicinal and aromatic plants of Turkiye-conservation and sustainable use. In: Full Text Proceedings Book (I. International Congress on Medicinal and Aromatic plants ‘Natural and Healthy Life’). 2017:11-39.
  • Kapdan E, Sezgin M. In vitro propagation to conserve the local endemic and endangered medicinal plant Helianthemum germanicopolitanum Bornm. Brazilian Archives of Biology and Technology 2021;64:1-14.
  • Coode MEJ. Helianthemum Adans. In: Davis PH, ed. Flora of Turkey and the East Aegean Islands, vol 1. Edinburgh University Press; 1965:506-512.
  • Lefebvre T, Destandau E, Lesellier E. Selective extraction of bioactive compounds from plants using recent extraction techniques: A review. J Chromatogr A 2021;1635:461770.
  • Ping W, Tinglan Z, Guohua Y, Mengjie L, Jin S, Jiaqi Z, et al. Poly-pharmacokinetic strategy-delineated metabolic fate of bioactive compounds in a traditional Chinese medicine formula, Yuanhu Zhitong tablets, using parallel reaction monitoring mode. Phytomedicine. 2019;53:53-61.
  • https://www.abcam.com/ps/products/65/ab65475/documents/Quick-Cell-Proliferation-Assay-Kit-II-Protocol-book-%20v2b-ab65475%20(website).pdf [cited 20 February 2024].
  • Kılıç AB, Karaman U, Yusufbeyoglu S, Yesilyurt EB, Yar TM. Investigation of in vitro anti-trichomoniasis effect of Helianthemum ledifolium L.(Mill.) varieties against Trichomonas vaginalis. Ann Med Res 2023;30(12):1557-62.
  • Mouffouk S, Mouffouk C, Mouffouk S, Haba H. Medicinal, pharmacological and biochemical progress on the study of genus Helianthemum: A review. Current Chemical Biology 2023;17(3):147-159.
  • Atınç M, Kalkan İ. Flavonoidler ve sağlık üzerine etkileri. Aydın Gastronomy 2018;2(1):31-38.
  • Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: A review. Tropical Journal of Pharmaceutical Research 2008;7(3):1089-1099.
  • Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M. Herbal antioxidant in clinical practice: A review. Asian Pac J Trop Biomed 2014;4(1):78-84.
  • Baldemir A, Gökşen N, Ildız N, Karatoprak GŞ, Koşar M. Phytochemical profile and biological activities of Helianthemum canum l. Baumg. from Turkey. Chem Biodivers 2017;14(7):10.1002/cbdv.201700052.
  • Muema FW, Liu Y, Zhang Y, Chen G, Guo M. Flavonoids from Selaginella doederleinii Hieron and their antioxidant and antiproliferative activities. Antioxidants (Basel) 2022;11(6):1189.
  • Zaidun NH, Thent ZC, Latiff AA. Combating oxidative stress disorders with citrus flavonoid: Naringenin. Life Sci 2018;208:111-122.
  • Terahara N. Flavonoids in foods: A review. Nat Prod Commun 2015;10(3):521-528.
  • Khongkaew P, Wattanaarsakit P, Papadopoulos KI, Chaemsawang W. Antioxidant effects and in vitro cytotoxicity on human cancer cell lines of flavonoid-rich flamboyant (Delonix regia (Bojer) Raf.) flower extract. Curr Pharm Biotechnol 2021;22(13):1821-1831.
  • Kamiloğlu S, Paslı AA, Çapanoğlu E, Özçelik B. Kuru meyvelerin kuruyemişler ile birlikte tüketiminin flavonoidlerin in vitro biyoyararlılığına etkisinin incelenmesi. Gıda 2014;39(4):227-233.
  • Viskupi ová , ndrejovi M, turd k E. Bioavailability and metabolism of flavonoids. ournal of Food Nutrition Research 2008;47(4).
  • Cahyana Y, Adiyanti T. Flavonoids as antidiabetic agents. Indonesian Journal of Chemistry 2021;21(2):512-526.
  • Pei R, Liu X, Bolling B. Flavonoids and gut health. Current Opinion in Biotechnology 2020;61:153-159.
  • Terfassi S, Dauvergne X, Cérantola S, Lemoine C, Bensouici C, Fadila B, Magné C, Marchioni E, Benayache S. First report on phytochemical investigation, antioxidant and antidiabetic activities of Helianthemum getulum. Natural Product Research. 2022;36(11):2806-2813.
  • Plescia F, Venturella F, D’Anneo A, Catania V, Gargano ML, Polito G, Schillaci D, Palumbo Piccionello A, Lauricella M, Venturella G, Raffa D. Phytochemical-rich extracts of Helianthemum lippii possess antimicrobial, anticancer, and anti-biofilm activities. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2022;156(6):1314-1324.
  • Kuntz S, Wenzel U, Daniel H. Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity, and apoptosis in human colon cancer cell lines. Eur J Nutr 1999;38(3):133-142.

Türkiye’nin Nadir Endemiği Helianthemum germanicopolitanum Bornm. Bitki Ekstraktının HT-29 Hücre Hattı Üzerindeki Sitotoksisite Analizi

Year 2024, Volume: 63 Issue: 3, 441 - 448, 09.09.2024

Kubilay Doğan Kılıç , Ayşenur Kayabaş Avşar , Cemre Özkanca , Dila Hatun Sal , Fatih Karabey , Zeynep Simge Yılmaz , Yiğit Uyanıkgil

https://doi.org/10.19161/etd.1441204

Abstract

Amaç: Bu çalışmada endemik Helianthemum germanicopolitanum Bornm. bitkisinin kolon kanserine karşı sitotoksik potansiyeli araştırılmıştır.
Gereç ve Yöntem: Bu çalışma, özellikle HT-29 insan kolon kanseri hücre hattını hedef alarak H. germanicopolitanum bitkisinin tıbbi uygulamalarının araştırılmasına öncülük etmektedir. Bitkinin topraküstü kısımlarının fitokimyasal profili, özellikle de flavonoid içeriği Yüksek Performanslı Sıvı Kromatografisi (HPLC) kullanılarak analiz edilmiştir. Sitotoksik etkiler daha sonra HT-29 hücre hattı üzerinde WST-1 deneyleri ile değerlendirilmiştir; bu, kanser hücresi büyümesinin zamana ve doza bağlı inhibisyonunu ortaya çıkarmıştır.
Bulgular: Flavonoid profillerinin ve sitotoksisitenin karşılaştırmalı analizleri, benzer türler üzerindeki mevcut literatüre göre yapılmıştır. Bu çalışmanın bulguları, H. germanicopolitanum’un kolon kanseri tedavisinde terapötik uygulamaları olan biyoaktif bileşiklerin kaynağı olarak tıbbi açıdan potansiyelinin belirlenmesine ışık tutmaktadır.
Sonuç: Bu sonuçlar ayrıca, H. germanicopolitanum bitkisinin eşsiz flavonoid bileşimi ve kanser tedavisindeki daha geniş etkileri hakkında kapsamlı araştırmalara duyulan ihtiyacı vurgulamaktadır.

Keywords

Helianthemum germanicopolitanum Kolon kanseri HT-29 Hücre Hattı Flavonoidler Sitotoksisite

References

  • Jain A, Jain P, Soni P, Tiwari A, Tiwari SP. Design and characterization of silver nanoparticles of different species of Curcuma in the treatment of cancer using human colon cancer cell line (HT-29). J Gastrointest Cancer 2023;54(1):90-5.
  • TSCRS (Turkish Society of Colon and Rectal Surgery). (2023). https://www.tkrcd.org.tr/en/ [cited 21 November 2023].
  • WHO (World Health Organization). (2023). https://www.who.int/news-room/fact-sheets/detail/colorectal-cancer?gclid=Cj0KCQiApOyqBhDlARIsAGfnyMpXMoYCnlou8M2JjUr3FnbCTQPCrGRl7bDBWM1fV16Ob9fmpINYiocaArbpEALw_wcB [cited 21 November 2023].
  • Karahan F. Evaluation of trace element and heavy metal levels of some ethnobotanically important medicinal plants used as remedies in Southern Turkey in terms of human health risk. Biol Trace Elem Res 2023; 201(1): 493-513.
  • Bordoloi C, Kumar S, Barbhuiya AM, Kushari S, Kalita JM, Sahu BP, Laloo D. Herbal medicine used for wound healing by the tribes of the North Eastern States of India: A comprehensive review. J Herbal Med 2023;100697.
  • Kına E, Uysal İ, Mohammed FS, Doğan M, Sevindik M. In-vitro antioxidant and oxidant properties of Centaurea rigida. Turk J Agric Food Sci Technol 2021;9(10):1905-7.
  • Gong P, Long H, Guo Y, Wang Z, Yao W, Wang J, et al. Chinese herbal medicines: The modulator of nonalcoholic fatty liver disease targeting oxidative stress. J Ethnopharmacol 2024;318(Pt B):116927.
  • Kuruppu AI, Paranagama P, Goonasekara CL. Medicinal plants commonly used against cancer in traditional medicine formulae in Sri Lanka. Saudi Pharm J 2019;27(4):565-573.
  • Máthé Á, Turgut K. Introduction to Medicinal and Aromatic Plants in Türkiye. In: Medicinal and Aromatic Plants of Turkey. Cham: Springer International Publishing;2023:1-30.
  • Ağca A, Yılmaz Sarıaltın S, Sever Yılmaz B. Evaluation of free radical scavenging, anti-inflammatory and hypoglycemic activity of Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 2023;26(2):227-233.
  • Küpeli Akkol E, Kosar M, Baldemir A, Şeker Karatoprak G, Demirpolat E, Yerer Aycan MB, et al. The wound-healing potential of the endemic plant Helianthemum canum (L.) Baumg: Preclinical studies supported with phytochemical profiling. Chem Biodivers 2023;20(12):e202301529.
  • Laib I, Djahra AB. Phytochemical investigation of Helianthemum lippii l. aerial Dum. Cours part and evaluation for its antioxidant activities. International Journal of Secondary Metabolite 2022;9(2):229-237.
  • Djemam N, Lassed S, Gül F, Altun M, Monteiro M, Menezes-Pinto D, et al. Characterization of ethyl acetate and n-butanol extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon. J Ethnopharmacol 2020;252:112613.
  • Güner A, Aslan S, Ekim T, Vural M, Babaç MT (Editörler). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını; 2012:370.
  • Yeşilyurt EB, Erik S, Özmen E, Akaydın G. Comparative morphological, palynological and anatomical characteristics of Turkish rare endemics Helianthemum germanicopolitanum and Helianthemum antitauricum (Cistaceae). Plant Systematics and Evolution 2015;301:125-137.
  • İnan E, İpek G, İpek A. Çankırı’nın Endemik Tıbbi Bitkileri. Türk Bilimsel Derlemeler Dergisi 2012;(2):38-40
  • Öztürk M, Altay V. An overview of the endemic medicinal and aromatic plants of Turkiye-conservation and sustainable use. In: Full Text Proceedings Book (I. International Congress on Medicinal and Aromatic plants ‘Natural and Healthy Life’). 2017:11-39.
  • Kapdan E, Sezgin M. In vitro propagation to conserve the local endemic and endangered medicinal plant Helianthemum germanicopolitanum Bornm. Brazilian Archives of Biology and Technology 2021;64:1-14.
  • Coode MEJ. Helianthemum Adans. In: Davis PH, ed. Flora of Turkey and the East Aegean Islands, vol 1. Edinburgh University Press; 1965:506-512.
  • Lefebvre T, Destandau E, Lesellier E. Selective extraction of bioactive compounds from plants using recent extraction techniques: A review. J Chromatogr A 2021;1635:461770.
  • Ping W, Tinglan Z, Guohua Y, Mengjie L, Jin S, Jiaqi Z, et al. Poly-pharmacokinetic strategy-delineated metabolic fate of bioactive compounds in a traditional Chinese medicine formula, Yuanhu Zhitong tablets, using parallel reaction monitoring mode. Phytomedicine. 2019;53:53-61.
  • https://www.abcam.com/ps/products/65/ab65475/documents/Quick-Cell-Proliferation-Assay-Kit-II-Protocol-book-%20v2b-ab65475%20(website).pdf [cited 20 February 2024].
  • Kılıç AB, Karaman U, Yusufbeyoglu S, Yesilyurt EB, Yar TM. Investigation of in vitro anti-trichomoniasis effect of Helianthemum ledifolium L.(Mill.) varieties against Trichomonas vaginalis. Ann Med Res 2023;30(12):1557-62.
  • Mouffouk S, Mouffouk C, Mouffouk S, Haba H. Medicinal, pharmacological and biochemical progress on the study of genus Helianthemum: A review. Current Chemical Biology 2023;17(3):147-159.
  • Atınç M, Kalkan İ. Flavonoidler ve sağlık üzerine etkileri. Aydın Gastronomy 2018;2(1):31-38.
  • Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: A review. Tropical Journal of Pharmaceutical Research 2008;7(3):1089-1099.
  • Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M. Herbal antioxidant in clinical practice: A review. Asian Pac J Trop Biomed 2014;4(1):78-84.
  • Baldemir A, Gökşen N, Ildız N, Karatoprak GŞ, Koşar M. Phytochemical profile and biological activities of Helianthemum canum l. Baumg. from Turkey. Chem Biodivers 2017;14(7):10.1002/cbdv.201700052.
  • Muema FW, Liu Y, Zhang Y, Chen G, Guo M. Flavonoids from Selaginella doederleinii Hieron and their antioxidant and antiproliferative activities. Antioxidants (Basel) 2022;11(6):1189.
  • Zaidun NH, Thent ZC, Latiff AA. Combating oxidative stress disorders with citrus flavonoid: Naringenin. Life Sci 2018;208:111-122.
  • Terahara N. Flavonoids in foods: A review. Nat Prod Commun 2015;10(3):521-528.
  • Khongkaew P, Wattanaarsakit P, Papadopoulos KI, Chaemsawang W. Antioxidant effects and in vitro cytotoxicity on human cancer cell lines of flavonoid-rich flamboyant (Delonix regia (Bojer) Raf.) flower extract. Curr Pharm Biotechnol 2021;22(13):1821-1831.
  • Kamiloğlu S, Paslı AA, Çapanoğlu E, Özçelik B. Kuru meyvelerin kuruyemişler ile birlikte tüketiminin flavonoidlerin in vitro biyoyararlılığına etkisinin incelenmesi. Gıda 2014;39(4):227-233.
  • Viskupi ová , ndrejovi M, turd k E. Bioavailability and metabolism of flavonoids. ournal of Food Nutrition Research 2008;47(4).
  • Cahyana Y, Adiyanti T. Flavonoids as antidiabetic agents. Indonesian Journal of Chemistry 2021;21(2):512-526.
  • Pei R, Liu X, Bolling B. Flavonoids and gut health. Current Opinion in Biotechnology 2020;61:153-159.
  • Terfassi S, Dauvergne X, Cérantola S, Lemoine C, Bensouici C, Fadila B, Magné C, Marchioni E, Benayache S. First report on phytochemical investigation, antioxidant and antidiabetic activities of Helianthemum getulum. Natural Product Research. 2022;36(11):2806-2813.
  • Plescia F, Venturella F, D’Anneo A, Catania V, Gargano ML, Polito G, Schillaci D, Palumbo Piccionello A, Lauricella M, Venturella G, Raffa D. Phytochemical-rich extracts of Helianthemum lippii possess antimicrobial, anticancer, and anti-biofilm activities. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2022;156(6):1314-1324.
  • Kuntz S, Wenzel U, Daniel H. Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity, and apoptosis in human colon cancer cell lines. Eur J Nutr 1999;38(3):133-142.
There are 39 citations in total.

Details

Primary Language English
Subjects Cancer Cell Biology, Cancer Therapy (Excl. Chemotherapy and Radiation Therapy)
Journal Section Research Articles
Authors

Kubilay Doğan Kılıç 0000-0002-9484-0777

Ayşenur Kayabaş Avşar 0000-0003-3555-4399

Cemre Özkanca 0000-0002-0342-2060

Dila Hatun Sal 0009-0007-3840-6347

Fatih Karabey 0000-0003-0305-6192

Zeynep Simge Yılmaz 0000-0002-1460-8530

Yiğit Uyanıkgil 0000-0002-4016-0522

Publication Date September 9, 2024
Submission Date February 26, 2024
Acceptance Date May 30, 2024
Published in Issue Year 2024 Volume: 63 Issue: 3

Cite

Vancouver Kılıç KD, Kayabaş Avşar A, Özkanca C, Sal DH, Karabey F, Yılmaz ZS, et al. Cytotoxicity assay of Turkish rare endemic Helianthemum germanicopolitanum Bornm. plant extract on HT-29 cell line. EJM. 2024;63(3):441-8.
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