Naringin CCl4 indüklü hepatosit hasarını endoplazmik retikulum stresini engelleyerek hafifletir

Umut Kerem Kolaç

doi:10.19161/etd.1133788

TR EN

Naringin CCl4 indüklü hepatosit hasarını endoplazmik retikulum stresini engelleyerek hafifletir

Öz

Amaç: Karaciğerin çeşitli kimyasallara maruz kalması hepatik hasar oluşmasına neden olur. Karbon tetraklorür (CCl4), kimyasal toksin kaynaklı hepatik hasarı araştırmak için yaygın şekilde kullanılmaktadır. Çalışmamızda turunçgillerde bol bulunan bir flavanon olan naringinin (NRG), CCl4 ile indüklenen karaciğer hasarında endoplazmik retikulum (ER) stresi ve stres aracılı apoptoz üzerine etkileri incelenmiştir. Gereç ve Yöntem: THLE-3 hücreleri 24 saat boyunca değişen konsantrasyonlarda CCl4’ e maruz bırakıldıktan sonra farklı dozlarda NRG ile 4 saat tedavi edildi. Konsantrasyonların hücre canlılığı üzerindeki etkileri belirlendi. Ardından hepatositlerde ER stres belirteçlerinin protein ifadeleri tespit edildi. Son olarak akış sitometri ile Bcl2 aktif / inaktif hücre oranları belirlendi. Bulgular: NRG tedavisi (5 ve 10 µM), CCl4 uygulaması ile azalan hücre canlılıklarında anlamlı bir artış sağladı. Benzer biçimde ER stres belirteçlerinde CCl4 uygulaması sonucu artan seviyeler NRG tedavisiyle anlamlı biçimde azaldı. Son olarak NRG, Bcl2 inaktif hücre oranını anlamlı seviyede düşürerek apoptozun önüne geçilmesinde faydalı oldu. Sonuç: NRG tedavisi CCl4 ile indüklenen hepatosit hasarında ER stresinin bastırılmasında ve ER stresi kaynaklı apoptozun önlenmesinde etkilidir.

Anahtar Kelimeler

Naringin attenuates CCl4-Induced hepatocyte damage through inhibiting endoplasmic reticulum stress

Abstract

Aim: Exposure of the liver to diverse chemicals induce hepatic damage. Carbon tetrachloride (CCl4) is widely used toxin to investigate hepatic injury. In our study, the effects of naringin (NRG), a flavanone abundant in citrus fruits, on endoplasmic reticulum (ER) stress and stress-mediated apoptosis in CCl4- induced liver injury were investigated. Materials and Methods: THLE-3 cells were exposed to varying concentrations of CCl4 for 24 hours and then treated with different doses of NRG for 4 hours. The effects of varying concentrations on cell viability were determined. Then, protein expressions of ER stress markers were detected in hepatocytes. Finally, Bcl2 active / inactive cell ratios were determined by flow cytometry. Results: NRG treatment (5 and 10 µM) resulted in a significant increase in cell viability, which decreased with CCl4 administration. Similarly, the increased levels of ER stress markers as a result of CCl4 application were significantly reduced with NRG treatment. Finally, NRG prevented apoptosis by significantly reducing ratio of Bcl2 inactive cells. Conclusion: NRG treatment is effective in suppressing ER stress in CCl4-induced hepatocyte damage and preventing ER stress-induced apoptosis.

Keywords

Supporting Institution

Çalışmayı destekleyen kurum bulunmamaktadır.

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Umut Kerem Kolaç ^*
0000-0003-0266-9069
Türkiye

Publication Date

March 15, 2023

Submission Date

June 21, 2022

Acceptance Date

July 18, 2022

Published in Issue

Year 2023 Volume: 62 Number: 1

DOI

https://doi.org/10.19161/etd.1133788

IZ

https://izlik.org/JA74RL53BD

Cite

RIS / Bibtex

Vancouver

1.Umut Kerem Kolaç. Naringin CCl4 indüklü hepatosit hasarını endoplazmik retikulum stresini engelleyerek hafifletir. EJM. 2023 Mar. 1;62(1):48-54. doi:10.19161/etd.1133788