The morphological analysis of anaplastic thyroid cancer cell line

Hatice Özışık; Berrin Ozdil; Merve Ozdemir; Murat Sipahi; Mehmet Erdoğan; Şevki Çetinkalp; Ahmet Gökhan Özgen; Füsun Saygılı; Gülgün Oktay; Hüseyin Aktuğ

doi:10.19161/etd.1168177

TR EN

Anaplastik tiroid kanseri hücre hattının morfolojik analizi

Öz

Amaç: Tiroid foliküler hücre kaynaklı kanserler papiller tiroid kanseri (%85), foliküler tiroid kanseri (%12) ve anaplastik tiroid kanseri (ATK) (%3) olmak üzere üç kategoriye ayrılır. ATK'lerin çok hızlı seyri, kötü tedavi sonuçları vardır ve çok agresiftirler. Bu çalışmanın amacı, ATK hücre hattının normal tiroid hücre hattı (NTH) ile olan morfolojik farklılıklarının analizini değerlendirmektir. Gereç ve Yöntem: NTH ve ATK hücrelerinde hematoksilen-eosin boyama ile inceleme yapıldı, nükleus sitoplazma (N:S) oranlarına bakıldı ve hücre döngüleri incelendi. Bu hücre hatları N:S oranlarına ve hücre döngüsü fazlarındaki bulunma oranlarına göre karşılaştırıldı. Bulgular: ATK hızlı seyri ve nadir görülmesi nedeniyle tedavisi zor bir kanser türüdür. Kültürlenmiş hücre hatlarında çalışmak, ATK hücrelerinin biyolojisindeki davranışları keşfetmek (hücre siklusu gibi), hastalığın seyrini anlamak ve tedavi için etkili bir hedef bulmak açısından önem taşımaktadır. Sonuç: N:S oranı ATK'de NTH'den daha yüksekti. Her iki hücre grubu da çoğunlukla G0/G1 fazında (68.4; 82.8) bulundu ve hem G0/G1 hem de S fazlarında istatistiksel farklılıklara sahipti.

Anahtar Kelimeler

The morphological analysis of anaplastic thyroid cancer cell line

Abstract

Aim: Thyroid follicular cell derived cancers are classified into three groups such as papillary thyroid cancer (85%), follicular thyroid cancer (12%) and anaplastic (undifferentiated) thyroid cancer (ATC) (3%). ATCs have very rapid course, poor treatment outcomes and they are very aggressive. The aim of current study was to assess the analysis of the morphological differences of ATC cell line with the normal thyroid cell line (NTC). Materials and Methods: NTH and ATC cells were examined with haematoxylin and eosin, the nucleus: cytoplasm (N:C) ratios were detected, and cell cycles were investigated. These cell lines were compared according to their N:C ratio and their abundance in cell cycle phases. Results: The N:C ratio was higher in ATC than NTC. Both cell groups were mostly found in G0/G1 phase (68.4; 82.8) and have statistical difference in both G0/G1 and S phases. Conclusion: The rapid course and the rarity of ATC are significant barriers for clinical trials. Cultured cell lines are very important to explore the behaviour in the biology of ATC cells (such as the cell cycle), to understand the course of the disease, and to find an effective target for treatment.

Keywords

Supporting Institution

yok

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Hatice Özışık ^*
0000-0003-4336-814X
Türkiye

Berrin Ozdil
0000-0001-6081-2308
Türkiye

Merve Ozdemir
0000-0003-2639-9980
Türkiye

Murat Sipahi
0000-0003-1993-6741
Türkiye

Mehmet Erdoğan
0000-0002-0300-3923
Türkiye

Şevki Çetinkalp
0000-0001-6072-2062
Türkiye

Ahmet Gökhan Özgen
0000-0002-4451-6530
Türkiye

Füsun Saygılı
0000-0002-4451-6530
Türkiye

Gülgün Oktay
0000-0002-0481-6029
Türkiye

Hüseyin Aktuğ
0000-0003-4150-8495
Türkiye

Publication Date

September 12, 2022

Submission Date

June 29, 2022

Acceptance Date

July 28, 2022

Published in Issue

Year 2022 Volume: 61 Number: 3

DOI

https://doi.org/10.19161/etd.1168177

IZ

https://izlik.org/JA94GZ74MB

Cite

RIS / Bibtex

Vancouver

1.Hatice Özışık, Berrin Ozdil, Merve Ozdemir, Murat Sipahi, Mehmet Erdoğan, Şevki Çetinkalp, Ahmet Gökhan Özgen, Füsun Saygılı, Gülgün Oktay, Hüseyin Aktuğ. The morphological analysis of anaplastic thyroid cancer cell line. EJM. 2022 Sep. 1;61(3):460-4. doi:10.19161/etd.1168177