Astım ve alerjik rinit komorbiditesi olan çocuklarda nazofaringeal mikrobiyotanın araştırılması

Cengiz Çavuşoğlu; Hasan Yüksel; Adem Yaşar; Tarık İnci; Furkan Polat; Ayça Aydın Uysal; Ayça Aykut

doi:10.19161/etd.1167440

TR EN

Astım ve alerjik rinit komorbiditesi olan çocuklarda nazofaringeal mikrobiyotanın araştırılması

Öz

Amaç: Çalışmada sağlıklı, astımlı ve alerjik rinitli çocukların nazofaringeal bakteriyel mikrobiyotasının karşılaştırılması, hastalarda olası mikrobiyal disbiyozisin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmaya toplam 15 olgu alınmıştır. Olguların beşi astımlı, beşi alerjik rinitli ve beşi sağlıklı kontrol grubudur. Nazal lavaj örneklerinden 16S metagenomiks ile üst solunum yolu mikrobiyotası belirlenmiştir. Bulgular: Üst solunum yolu mikrobiyotasında en baskın şube astım hastalarında Firmucutes, sağlıklı kontrol grubu ve alerjik rinit grubunda ise Proteobacteria olarak saptanmıştır. Üst solunum yolu mikrobiyotasındaki en baskın cins ise astım hastalarında Dolosigranulum, sağlıklı kontrol grubunda Moraxella olarak saptanmıştır. Kontrol grubuyla karşılaştırıldığında astım hastalarında Moraxella cinsinin oranın azaldığı; Staphylococcus, Streptococcus ve Corynebacterium cinslerinin oranlarının arttığı belirlenmiştir. Sonuç: Sonuç olarak; çocukluk çağında üst solunum yolu mikrobiyotasının alerjik rinit ve astım patogenezini belirlemedeki rolü kesin olarak saptanamamıştır. Gruplar arası oransal fark bulunması, tüm havayolu mikrobiyomunun çalışılması durumunda olası bir farkın olabileceğini desteklemektedir.

Anahtar Kelimeler

Investigation of nasopharyngeal microbiota in children with asthma and allergic rhinitis comorbidity

Abstract

Aim: The goal of this study was to compare the nasopharyngeal bacterial microbiota of healthy children with asthma and allergic rhinitis, identify potential microbial dysbiosis in patients. Materials and Methods: The study included a total of 15 patients. There were five patients with asthma, five with allergic rhinitis, and five healthy controls. The upper respiratory tract microbiota were identified using 16S metagenomics analysis of nasal lavage samples. Results: Firmucutes was the most prevalent phylum in the upper respiratory tract microbiota of asthma patients, while Proteobacteria were found in the healthy control and allergic rhinitis groups. Dolosigranulum was identified as the most dominant genus in the upper respiratory tract microbiota of asthma patients. Moraxella was the most prevalent genera in the upper respiratory tract microbiota of the healthy control group. When asthma patients were compared to the control group, the ratio of the Moraxella genus decreased while the ratios of Staphylococcus, Streptococcus, and Corynebacterium species increased. Conclusion: In conclusion, it has not been determined that upper respiratory tract microbiota has a role in determining the pathogenesis of allergic rhinitis and asthma in childhood. The fact that there is a proportional difference between groups’ supports that there may be a possible difference if the entire airway microbiome is studied.

Keywords

References

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Cengiz Çavuşoğlu ^*
0000-0001-7152-5216
Türkiye

Hasan Yüksel
0000-0001-9577-8445
Türkiye

Adem Yaşar
Türkiye

Tarık İnci
0000-0002-5139-1262
Türkiye

Furkan Polat
0000-0003-4640-3991
Türkiye

Ayça Aydın Uysal
0000-0003-0192-7126
Türkiye

Ayça Aykut
0000-0002-1460-0053
Türkiye

Publication Date

September 12, 2022

Submission Date

September 15, 2021

Acceptance Date

March 8, 2022

Published in Issue

Year 2022 Volume: 61 Number: 3

DOI

https://doi.org/10.19161/etd.1167440

IZ

https://izlik.org/JA42NT83MP

Cite

RIS / Bibtex

Vancouver

1.Cengiz Çavuşoğlu, Hasan Yüksel, Adem Yaşar, Tarık İnci, Furkan Polat, Ayça Aydın Uysal, Ayça Aykut. Astım ve alerjik rinit komorbiditesi olan çocuklarda nazofaringeal mikrobiyotanın araştırılması. EJM. 2022 Sep. 1;61(3):371-8. doi:10.19161/etd.1167440