Effects of hyperbaric oxygen therapy on the morphological characteristics and survival of MCF-7 breast cancer cells

Gürkan Yiğittürk; Serkan Ergözen; Hülya Elbe; Anıl Yücel; Türker Çavuşoğlu; Tuba Baygar; Yiğit Uyanıkgil

doi:10.19161/etd.1235892

EN TR

Effects of hyperbaric oxygen therapy on the morphological characteristics and survival of MCF-7 breast cancer cells

Abstract

Aim: This study aims to determine the effects of hyperbaric oxygen therapy at different pressure values on cell morphology and cell survival in the MCF-7 breast cancer cell line. Materials and Methods: The experimental groups were formed by applying 100% oxygen to MCF-7 breast cancer cells at 1.5, 2, and 2.5 atmospheres for 2 hours. The control group did not receive treatment. At the end of the experiment, cell survival was investigated by CCK-8 analysis, cell shapes were determined by cresyl violet staining, and cell surface morphologies were determined by scanning electron microscope. Results: Cell viability was significantly reduced at atmospheric pressure of 1.5, 2, and 2.5 compared to the control group (p < 0.005). As pressure increased, the surface area of the cell decreased, nuclear condensation increased, and the cell borders became irregular. Cell membrane bleb and cell membrane porosity increased at 2 and 2.5 atmospheres. Conclusion: Hyperbaric oxygen therapy severely reduces the viability of MCF-7 breast cancer cells under increased pressure. It can induce apoptosis and change the shape and surface morphology of MCF-7 breast cancer cells. Although further studies are needed, our study supports the potential use of hyperbaric oxygen therapy in the treatment of breast cancer.

Keywords

Hiperbarik oksijen tedavisinin MCF-7 meme kanseri hücrelerinin morfolojik özellikleri ve sağkalımı üzerindeki etkileri

Öz

Amaç: Bu çalışma, MCF-7 meme kanseri hücre hattına farklı basınçlar altında uygulanan hiperbarik oksijen tedavisinin hücre morfolojisi ve hücre sağkalımı üzerindeki etkilerini belirlemeyi amaçlamaktadır. Gereç ve Yöntem: MCF-7 meme kanser hücrelerine 1,5, 2 ve 2,5 atmosfer basınç altında 2 saat boyunca %100 oksijen uygulanarak deney grupları oluşturuldu. Kontrol grubuna ise herhangi bir tedavi uygulanmadı. Deney sonunda; hücre sağkalımları CCK-8 analizi hücre şekilleri cristal viyole boyama ve hücre yüzey morfolojileri taramalı elektron mikroskobu ile belirlendi. Bulgular: Hücre canlılığı, kontrol grubuna kıyasla 1,5, 2 ve 2,5 atmosfer basıncında önemli ölçüde azaldı (p < 0.005). Basınç arttıkça hücre yüzey alanının azaldığı, nükleer yoğunlaşmanın arttığı ve hücre sınırlarının düzensizleştiği görüldü. İki ve 2,5 atmosfer basınçta, hücre zarının kabarcık şekilli protrüzyonu ve orasyonunda artış tespit edildi. Sonuç: Hiperbarik oksijen tedavisi artan basınçla orantılı olarak MCF-7 meme kanseri hücrelerinin canlılığını ciddi şekilde azaltır. Hücre şeklini ve yüzey morfolojilerini değiştirir. MCF-7 meme kanseri hücrelerinde apoptozu tetikleyebilir. Daha ileri çalışmalara ihtiyaç duyulmasına rağmen, çalışmamız meme kanseri tedavisinde hiperbarik oksijen tedavisinin kullanım potansiyelini desteklemektedir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Gürkan Yiğittürk ^*
0000-0002-5315-253X
Türkiye

Serkan Ergözen
0000-0003-1171-3703
Türkiye

Hülya Elbe
0000-0002-1254-0683
Türkiye

Anıl Yücel
0000-0002-6018-2737
Türkiye

Türker Çavuşoğlu
0000-0001-7100-7080
Türkiye

Tuba Baygar
0000-0002-1238-3227
Türkiye

Yiğit Uyanıkgil
0000-0002-4016-0522
Türkiye

Publication Date

March 15, 2023

Submission Date

October 5, 2022

Acceptance Date

December 27, 2022

Published in Issue

Year 2023 Volume: 62 Number: 1

DOI

https://doi.org/10.19161/etd.1235892

IZ

https://izlik.org/JA64LS37EN

Cite

RIS / Bibtex

Vancouver

1.Gürkan Yiğittürk, Serkan Ergözen, Hülya Elbe, Anıl Yücel, Türker Çavuşoğlu, Tuba Baygar, Yiğit Uyanıkgil. Effects of hyperbaric oxygen therapy on the morphological characteristics and survival of MCF-7 breast cancer cells. EJM. 2023 Mar. 1;62(1):12-7. doi:10.19161/etd.1235892