Cilde uygulanan ağrılı elektrik uyarısıyla üst ekstremite kaslarında oluşan eşzamanlı refleks yanıtların elektromiyografi ile incelenmesi

Nasim Eftekhari; Mehmet Cemal Kahya; Sibel Kocaaslan Atlı

doi:10.19161/etd.1481645

EN TR

Examination of simultaneous reflex responses in the upper extremity muscles by recording electromyography with painful electrical stimulation on the skin

Abstract

Materials and Methods: Records of the single motor unit and surface electromyography were obtained by administering painful electrical stimulation to the dorsal surface of the dominant hand of nine healthy participants. The electrical stimulus, delivered in the form of a 0,2 ms square pulse, was randomly applied at intervals of 1-2 seconds. Averages are computed throughout 400 ms before and 500 ms after the stimulus. Analyses were conducted by selecting 16 suitable motor units from among the recordings. Reflex latency and duration were obtained from the cumulative sum graphs of surface electromyography, peristimulus time histogram, and peristimulus frequencygram analyses. Results: A cutaneous silent period was observed in all motor units of the dominant arm. According to the peristimulus frequencygram approach, the inhibition time observed in motor units was found to be 94.8 ms longer than that found by the peristimulus time histogram approach (p<0,05). Excitation was observed in both the histogram and surface electromyography, but not in the frequencygram. Inhibition was observed in the muscle on the dominant side, but not on the other side. Conclusion: The peristimulus frequencygram method has been shown in the literature to sample the postsynaptic potential developed on a motor nöron. In our study, the finding of long inhibition with the frequencygram method has supported that inhibitory circuits in the medulla spinalis create long-term inhibition. The excitation observed in the peristimulus time histogram was not seen in the peristimulus frequencygram analysis. We recommend the use of the frequencygram method in addition to classical methods in reflex examinations.

Keywords

Cilde uygulanan ağrılı elektrik uyarısıyla üst ekstremite kaslarında oluşan eşzamanlı refleks yanıtların elektromiyografi ile incelenmesi

Öz

Amaç: Bu araştırmada, sağlıklı bireylerde ağrılı elektriksel uyarımın bilateral m. brachioradialis kasındaki refleks yanıtını klasik analiz ve frekans analiz yöntemlerini birlikte kullanarak incelemek amaçlanmıştır. Gereç ve Yöntem: Dokuz sağlıklı katılımcının dominant el sırtına ağrılı elektrik uyaranı verilerek tek motor ünite ve yüzeyel elektromiyografi kayıtları alındı. Elektrik uyarısı 0,2 ms süreli kare puls şeklinde, 1-2 saniye aralıklarla rastgele uygulandı. Uyarıdan 400 ms öncesi ve 500 ms sonrasındaki kısmın ortalaması alındı. Kayıtlarda 16 motor ünite seçilerek analizi yapıldı. Yüzeyel elektromiyografi, peristimulus time histogram ve peristimulus frekansgram analizlerinin kümülatif toplam grafiklerinden refleks latans ve süre bilgileri elde edildi. Bulgular: Dominant kolda motor ünitelerin hepsinde kütanöz sessiz periyot gözlemlendi. Peristimulus frekansgram’da motor ünitelerde gözlenen inhibisyon süresi peristimulus time histograma göre 94,8 ms daha uzun olduğu görüldü (p<0,05). Histogram ve yüzeyel elektromiyografide eksitasyon görülürken, frekansgramda gözlenmedi. Dominant taraftaki kasta inhibisyon gözlemlenirken diğerinde görülmedi. Sonuçlar: Peristimulus frekansgram yöntemi motor nöronda oluşan postsinaptik potansiyeli doğru bir şekilde gösterir. Araştırmamızda frekansgram yöntemiyle inhibisyonun uzun bulunması medulla spinaliste inhibitör devrelerin uzun süreli inhibisyon oluşturduğunu desteklemiştir. Peristimulus time histogramda görülen eksitasyon peristimulus frekansgram analizinde görülmedi. Refleks incelemelerinde klasik yöntemlere ek olarak frekansgram yönteminin de kullanılması uygun olacaktır. NOT: Bu çalışmanın ön raporu, 6-9 Eylül 2023 tarihleri arasında Türkiye Biyofizik Derneği tarafından İzmir Teknoloji Enstitüsü'nde düzenlenen 5. Uluslararası/34. Ulusal Biyofizik Kongresi’nde poster bildiri olarak sunulmuştur.

Anahtar Kelimeler

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, İzmir Kâtip Çelebi Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

1002-A Hızlı Destek 123S213, 2023-TYL-SABE-0019

Thanks

Yazarlar makalenin kritik değerlendirmesi ve katkıları için Prof. Dr. Kemal Sıtkı Türker’e teşekkür eder. Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu tarafından 1002-A Hızlı Destek kapsamında 123S213 Numaralı proje ve İzmir Kâtip Çelebi Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından 2023-TYL-SABE-0019 proje numarası ile desteklenmiştir. Projeye verdikleri destekten ötürü teşekkürlerimizi sunarız.

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Details

Primary Language

Turkish

Subjects

Human Biophysics

Journal Section

Research Article

Authors

Nasim Eftekhari
0000-0002-6553-6004
Türkiye

Mehmet Cemal Kahya ^*
0000-0002-3920-0574
Türkiye

Sibel Kocaaslan Atlı
0000-0002-7604-3870
Türkiye

Publication Date

March 12, 2025

Submission Date

May 13, 2024

Acceptance Date

October 8, 2024

Published in Issue

Year 2025 Volume: 64 Number: 1

DOI

https://doi.org/10.19161/etd.1481645

IZ

https://izlik.org/JA88KX46JS

Cite

RIS / Bibtex

Vancouver

1.Nasim Eftekhari, Mehmet Cemal Kahya, Sibel Kocaaslan Atlı. Cilde uygulanan ağrılı elektrik uyarısıyla üst ekstremite kaslarında oluşan eşzamanlı refleks yanıtların elektromiyografi ile incelenmesi. EJM. 2025 Mar. 1;64(1):21-3. doi:10.19161/etd.1481645