Effects of antioxidants used in the experimental premature ovarian insufficiency model on mitophagy proteins and them-associated MicroRNAs

Duygu Uçar Kartal; Naci Kemal Kuşçu; Kemal Özbilgin; Muhammed Yusuf Pekmezci

doi:10.19161/etd.1704479

TR EN

Deneysel prematüre yumurtalık yetmezliği modelinde kullanılan antioksidanların mitofaji proteinleri ve bunlarla ilişkili MikroRNA'lar üzerindeki etkileri

Öz

Amaç: Deneysel olarak Prematür Ovaryen Yetmezlik (POI) oluşturulmuş sıçanlar üzerinde; quercetin ve curcumin ile parkin bağımlı ve bağımsız mekanizmalarda meydana gelen değişiklikler inceledi. Folikül sayısı, immünohistokimyasal proteinler, mRNA'lar ve bunlarla ilişkili mikroRNA'ları inceleyerek POI'de quercetin ve curcuminin koruyucu ve tedavi edici etkilerini araştırmak amaçlandı. Gereç ve Yöntem: Çalışmamızda sıçanlarda siklofosfamid aracılığıyla deneysel prematüre over yetmezliği modeli oluşturuldu. Daha sonra dokular histopatolojik yöntemler ve histokimyasal boyama ile incelendi. Over örneklerinde RNA izolasyonu yapıldı ve cDNA sentezi ve qPCR işlemleri gerçekleştirildi. Bulgular: Elde edilen bulgular ile gruplar arasındaki fark istatistiksel olarak değerlendirildi.

Anahtar Kelimeler

Effects of antioxidants used in the experimental premature ovarian insufficiency model on mitophagy proteins and them-associated MicroRNAs

Abstract

Aim: It was aimed to investigate the protective and therapeutic effects of quercetin and curcumin in POI by examining follicle count, immunohistochemical proteins, mRNAs and their related microRNAs. Materials and Methods: In our study, an experimental premature ovarian insufficiency model was created on rats by means of cyclophosphamide. Then the tissues were examined by histopathological methods and histochemical staining. We studied the changes in parkin-dependent and independent mechanisms that occur with quercetin and curcumin. RNA isolation was performed on ovarian samples and cDNA synthesis, and qPCR processes were performed. The difference between the obtained findings and the groups was evaluated statistically. Results: Cyclophosphamide depleted primordial follicles, which is the main pool for fertility, on ovarian tissue and induced atresia (p<0.001). Curcumin and quercetin acted on parkin-dependent pathways, resulting in significant improvement in the ovarian follicle pool (p<0.001). Conclusion: The significant increase in the primordial follicle pool and the decrease in the rate of atretic follicles observed in the groups given curcumin and quercetin indicate that curcumin and quercetin have protective and therapeutic roles in the pathogenesis of POI by regulating mitophagy in the parkin-mediated pathway on the ovary.

Keywords

References

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Details

Primary Language

English

Subjects

Obstetrics and Gynaecology

Journal Section

Research Article

Authors

Duygu Uçar Kartal ^*
0000-0003-4751-5944
Türkiye

Naci Kemal Kuşçu
0000-0002-7953-9361
Türkiye

Kemal Özbilgin
0000-0001-6627-5443
Türkiye

Muhammed Yusuf Pekmezci
0000-0003-1040-0393
Türkiye

Publication Date

December 8, 2025

Submission Date

May 22, 2025

Acceptance Date

August 28, 2025

Published in Issue

Year 2025 Volume: 64 Number: 4

DOI

https://doi.org/10.19161/etd.1704479

IZ

https://izlik.org/JA77PY77RT

Cite

RIS / Bibtex

Vancouver

1.Duygu Uçar Kartal, Naci Kemal Kuşçu, Kemal Özbilgin, Muhammed Yusuf Pekmezci. Effects of antioxidants used in the experimental premature ovarian insufficiency model on mitophagy proteins and them-associated MicroRNAs. EJM. 2025 Dec. 1;64(4):688-700. doi:10.19161/etd.1704479