In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction

Nilgün Öksel; Mustafa Şanlı; Muzaffer Katar

doi:10.19161/etd.1929918

TR EN

Preeklamptik serum ile indüklenen endotel disfonksiyonunda Kafeik Asit Fenetil Esterin (CAPE) in vitro etkileri

Abstract

Amaç: Bu çalışmanın amacı, propolisin aktif bileşenlerinden biri olan kafeik asit fenetil esterin (CAPE), preeklampsi tanısı almış gebelerden elde edilen serum ile indüklenen in vitro endotel disfonksiyon modelinde endotel hücre fonksiyonları üzerindeki etkilerini araştırmaktır. Gereç ve Yöntem: Preeklampsi tanısı almış kadınlardan elde edilen %10 oranındaki serum (PE) kullanılarak HUVEC’lerde in vitro endotel disfonksiyon modeli oluşturuldu ve endotel hücre yanıtları %10 fetal bovine serum (FBS) ve normotansif gebelerden elde edilen %10 serum (N) ile kültüre edilen hücrelerle karşılaştırıldı. Kafeik asit fenetil esterin PE ile indüklenen endotel disfonksiyonu üzerindeki etkilerini değerlendirmek amacıyla, sitotoksik olmayan CAPE dozları PrestoBlue hücre canlılığı testi ile belirlendi ve elde edilen sonuçlara göre 10 ve 25 µM CAPE konsantrasyonları seçildi. Endotel hücre fonksiyonları, PE ile indüklenen endotel disfonksiyonu koşullarında CAPE’nin koruyucu etkilerini değerlendirmek amacıyla yara iyileşme (migrasyon) ve tüp oluşumu deneyleri ile analiz edildi. Bulgular: HUVEC’lerin PE serum ile muamelesi, FBS ve N gruplarıyla karşılaştırıldığında hücre canlılığını azalttı. PE ile muamele edilen HUVEC’lerde 10 ve 25 µM CAPE uygulaması hücre canlılığında istatistiksel olarak anlamlı bir değişikliğe yol açmadı. Fonksiyonel deneylerde, PE serum endotel migrasyonunu ve tüp oluşum kapasitesini belirgin şekilde bozdu. CAPE uygulaması, PE serum ile muamele edilen HUVEC’lerde yara kapanmasını ve anjiyojenik tüp oluşumunu anlamlı şekilde artırdı. Sonuç: CAPE, preeklamptik seruma maruz bırakılan endotel hücrelerinde migrasyon ve tüp oluşumunu artırarak, PE serum ile indüklenen endotel fonksiyon bozukluklarını iyileştirmiştir. Bu bulgular, CAPE’nin preeklampsi koşullarında endotel bütünlüğünün korunmasında potansiyel bir role sahip olabileceğini göstermektedir.

Keywords

Supporting Institution

Bu çalışma için herhangi bir kurum veya kuruluştan finansal destek alınmamıştır.

Ethical Statement

Tokat Gaziosmanpaşa Üniversitesi Müdahale Olmayan Bilimsel Araştırmalar Etik Kurulu (26-MOBAEK-031).

In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction

Abstract

Aim: The aim of study was to evaluate the potential protective effects of caffeic acid phenethyl ester (CAPE), one of the major bioactive components of propolis, within an in vitro model of preeclampsia-induced endothelial dysfunction. Materials and Methods: An in vitro endothelial dysfunction was modeled by treating Human Umbilical Vein Endothelial Cells (HUVECs) with serum from preeclamptic women (PE) and endothelial responses were then compared to normotensive pregnancy serum (N) and fetal bovin serum (FBS), which functioned as physiological and standard controls, respectively. To evaluate the effects of CAPE on PE serum-induced endothelial dysfunction, non-cytotoxic concentrations were first determined via the PrestoBlue cell viability assay. Based on these results, 10 and 25 µM CAPE were selected for subsequent experiments. The migratory and angiogenic capabilities of HUVECs were analyzed via wound healing and tube formation experiments to investigate the protective role of CAPE against preeclampsia-associated endothelial impairment. Results: Exposure to PE serum led to a significant reduction in HUVEC viability compared to FBS and N control groups. CAPE treatment at 10 and 25 µM did not significantly alter cell viability in PE serum treated HUVECs. In functional assays, PE serum markedly impaired both endothelial migration and tube formation capacity. CAPE treatment significantly attenuated these impairments, as evidenced by enhanced wound closure and restored angiogenic tube formation in PE-treated HUVECs. Conclusions: Our data demonstrate that CAPE treatment effectively counteracts PE serum-mediated endothelial impairment by promoting cell migration and angiogenic tube formation. These observations highlight the potential role of CAPE in maintaining endothelial integrity during preeclamptic conditions.

Keywords

Supporting Institution

No financial support was received for this study.

Ethical Statement

This study was approved by the Non-Interventional Scientific Research Ethics Committee of Tokat Gaziosmanpaşa University (Approval No: 26-MOBAEK-031).

References

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Details

Primary Language

English

Subjects

Clinical Sciences (Other)

Journal Section

Research Article

Authors

Nilgün Öksel ^*
0000-0003-4150-1680
Türkiye

Mustafa Şanlı
0009-0007-2542-5207
Türkiye

Muzaffer Katar
0000-0002-6296-2390
Türkiye

Publication Date

September 3, 2026

Submission Date

April 14, 2026

Acceptance Date

April 23, 2026

Published in Issue

Year 2026 Volume: 65 Number: 3

DOI

https://doi.org/10.19161/etd.1929918

IZ

https://izlik.org/JA36LS49AK

Cite

RIS / Bibtex

APA

Öksel, N., Şanlı, M., & Katar, M. (2026). In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction. Ege Tıp Dergisi, 65(3), 434-440. https://doi.org/10.19161/etd.1929918

AMA

1.Öksel N, Şanlı M, Katar M. In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction. EJM. 2026;65(3):434-440. doi:10.19161/etd.1929918

Chicago

Öksel, Nilgün, Mustafa Şanlı, and Muzaffer Katar. 2026. “In Vitro Effects of Caffeic Acid Phenethyl Ester (CAPE) on Preeclamptic Serum-Induced Endothelial Dysfunction”. Ege Tıp Dergisi 65 (3): 434-40. https://doi.org/10.19161/etd.1929918.

EndNote

Öksel N, Şanlı M, Katar M (September 1, 2026) In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction. Ege Tıp Dergisi 65 3 434–440.

IEEE

[1]N. Öksel, M. Şanlı, and M. Katar, “In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction”, EJM, vol. 65, no. 3, pp. 434–440, Sept. 2026, doi: 10.19161/etd.1929918.

ISNAD

Öksel, Nilgün - Şanlı, Mustafa - Katar, Muzaffer. “In Vitro Effects of Caffeic Acid Phenethyl Ester (CAPE) on Preeclamptic Serum-Induced Endothelial Dysfunction”. Ege Tıp Dergisi 65/3 (September 1, 2026): 434-440. https://doi.org/10.19161/etd.1929918.

JAMA

1.Öksel N, Şanlı M, Katar M. In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction. EJM. 2026;65:434–440.

MLA

Öksel, Nilgün, et al. “In Vitro Effects of Caffeic Acid Phenethyl Ester (CAPE) on Preeclamptic Serum-Induced Endothelial Dysfunction”. Ege Tıp Dergisi, vol. 65, no. 3, Sept. 2026, pp. 434-40, doi:10.19161/etd.1929918.

Vancouver

1.Nilgün Öksel, Mustafa Şanlı, Muzaffer Katar. In vitro effects of Caffeic Acid Phenethyl Ester (CAPE) on preeclamptic serum-induced endothelial dysfunction. EJM. 2026 Sep. 1;65(3):434-40. doi:10.19161/etd.1929918