ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir

Şule Ayla; Gülperi Öktem; Cüneyd Parlayan

doi:10.19161/etd.418162

TR EN

ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir

Öz

Amaç: Prostat kanser hücre hattı (DU145) ve prostat normal epitel hücre hatları (RWPE) arasında anoikis mekanizmasını arttıracak veya inhibe edebilecek genlerin analizini yapmak ve kanser gelişiminde olası rolünü incelemek.
Gereç ve Yöntem: İnsan prostat epitel hücre hattı (RWPE) ve prostat kanseri hücre hatları (DU-145) Amerikan Tip Kültür Koleksiyonu (ATCC)’den temin edildi. Hücre hatlarının çoğaltılmasında ve sürdürülmesinde RPMI 1640 (Biological Industries) besi ortamı kullanıldı. Transkriptom analizi için RNA izolasyonu yapılarak, kütüphane oluşturuldu, kütüphanenin kantitasyonunun ardından NextSeq500 (illumina) ile sekanslama yapıldı. Dizileme, haritalandırma, bağıl gen ifadeleri ölçümleri gibi biyoinformatik analizler Genomics Workbench v 8 (Qiagen) yazılımı kullanılarak GRCh38 referans sekansı ile yapılmıştır.

Bulgular: RWPE Normal prostat epitel hücre kültürleri ile DU145 prostat kanser hücreleri karşılaştırıldığı zaman DU-145 prostat kanser hücre kültürlerinde, ZNF304, PYCARD ve Notch3 gen expresyonlarında anlamlı bir artış (p<0,05) görülürken, CXCR4, Pak3, SerpınB1 gen ekspresyonlarında anlamlı bir azalma (p<0,05) görülmüştür.
Sonuç: DU145 prostat kanseri hücre hattında anoikis ile ilişkili önemli gen ekpresyonlarında artış ve azalma gözlemledik. Değişime bağlı olarak hücrelerin anoikisden kaçarak metastatik özellik kazanabileceğini düşündük.

Anahtar Kelimeler

Apoptozis,anoikis,kanser,mezenkimal geçiş.

References

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Şule Ayla ^*
0000-0003-2143-5268
Türkiye

Gülperi Öktem
0000-0001-6227-9519
Türkiye

Cüneyd Parlayan
0000-0002-6183-9489
Türkiye

Publication Date

September 14, 2018

Submission Date

July 16, 2017

Acceptance Date

July 24, 2017

Published in Issue

Year 1970 Volume: 57 Number: 3

DOI

https://doi.org/10.19161/etd.418162

IZ

https://izlik.org/JA23TM22SZ

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Vancouver

1.Şule Ayla, Gülperi Öktem, Cüneyd Parlayan. ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir. EJM. 2018 Sep. 1;57(3):157-62. doi:10.19161/etd.418162

Significant increase in ZNF304 and decrease in CXCR4 gene expressions may alter anoikis in prostate cancer

ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir

Öz

Anahtar Kelimeler

Significant increase in ZNF304 and decrease in CXCR4 gene expressions may alter anoikis in prostate cancer

Abstract

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