Cell-free supernatants of lactobacilli inhibit methicilin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and carbapenem-resistant Klebsiella strains

Fatma Kalaycı Yüksek; Defne Gümüş; Derya Bayırlı Turan; Yasar Nakipoğlu; Rıza Adaleti; A. Mine Küçüker

doi:10.19161/etd.1037310

EN TR

Cell-free supernatants of lactobacilli inhibit methicilin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and carbapenem-resistant Klebsiella strains

Abstract

Aim: Antibiotic resistance is a major health problem. Recently, probiotics are used in the field of alternative/supportive medicine. Thus, in this study, we aimed to evaluate the in vitro inhibitory effects of four different cell-free supernatants (CFSs) of lactobacilli species (L. fermentum, L. plantarum, L. acidophilus and L. rhamnosus) on clinically isolated Methicilin-resistant Staphylococcus aureus (MRSA) strains, Vancomycin-resistant Enterococcus (VRE) strains, and Carbapenem-resistant Klebsiella (CRK) strains. Materials and Methods: Lactobacillus strains were grown in de Man Rogosa Sharpe broth; after filtration, CFSs were diluted to obtain 25, 50, and 100% concentrations. Pathogen bacteria were grown in tryptic soy broth with and without CFSs in a micro-plate. The bacterial growths were measured using spectrophotometric method after four hours of incubation at 37°C. One-way ANOVA followed by Dunnett’s multiple comparisons and Kruskal-Wallis test were used for statistical analyses. Results: All tested CFSs at all concentrations were found to inhibit growth of MRSA, VRE, CRK strains; the results were found statistically significant (p<0.0001). At 50% concentrations, all CFSs were found to be most effective on MRSA growth. The CFSs of L.fermentum, L.acidophilus and L.plantarum were found to be most inhibitory at 50% concentration on VRE growth. 50% diluted CFSs of L.fermentum and L.plantarum were found to be effective on growth of CRK. All results were found statistically significant (p<0.0001). Conclusion: In our study, our results support that CFSs of lactobacilli strains inhibit growth of multi-drug resistant bacteria. Their inhibitory effects were dependent on microorganisms and CFS concentrations.

Keywords

Laktobasillerden elde edilen hücresiz süzüntülerin metisiline dirençli Staphylococcus aureus, vankomisine dirençli Enterococcus ve karbapeneme dirençli Klebsiella suşlarını inhibisyonu

Öz

Amaç: Antibiyotik direnci büyük bir sağlık sorunudur. Son yıllarda, probiyotikler alternatif/destekleyici tıp alanında sık kullanılmaktadır. Bu nedenle, çalışmamızda, dört farklı lactobacillus türünün (L. fermentum, L. plantarum, L. acidophilus ve L. rhamnosus) hücresiz süzüntülerinin (cell-free supernatant, CFS) etken olarak izole edilen metisiline dirençli Staphylococcus aureus (MRSA), vankomisine dirençli Enterococcus (VRE) ve karbapeneme dirençli Klebsiella (KRK) suşlarının üremeleri üzerine inhibitör etkilerini in vitro saptamayı amaçladık. Gereç ve Yöntem: Lactobacillus suşları de Man Rogosa Sharpe sıvı besiyerinde üretilmiştir; hücresiz süzüntüler %25, 50 ve 100’lük konsantrasyonları elde etmek için sulandırılmıştır. Patojen bakteriler mikroplaklarda, farklı konsantrasyonlarda CFS içeren ve içermeyen triptik soy buyyonda üretilmişlerdir. Üremeler, 37°C’deki dört saatlik inkübasyon sonunda spektrofotometrik yöntemle ölçülmüştür. İstatistiksel analizler, one-way ANOVA kullanılarak, Dunnett’in çoklu karşılaştırma testi ve Kruskal-Wallis test ile gerçekleştirilmiştir. Bulgular: İncelenen tüm CFS konsantrasyonlarının MRSA, VRE ve KRK suşlarının üremelerini inhibe ettiği saptanmış; sonuçlar istatistiksel olarak anlamlı bulunmuştur (p<0.0001). Tüm CFS’lerin %50’lik konsantrasyonlarının MRSA suşlarının üremesine en etkili konsantrasyon olduğu belirlenmiştir. L.acidophilus, L.fermentum ve L.plantarum CFS’lerinin %50’lik konsantrasyonlarının VRE suşlarının üremesine en etkili konsantrasyon olduğu bulunmuştur. L.fermentum ve L.plantarum CFS’lerinin %50’lik konsantrasyonlarının, KRK üremesini en etkili biçimde inhibe eden konsantrasyon olduğu saptanmıştır. Tüm sonuçlar istatistiksel olarak anlamlı bulunmuştur (p<0.0001). Sonuç: Çalışmamızda sonuçlarımız denenen tüm laktobasil CFS’lerinin çoğul antibiyotik dirençli bakterilerin üremesini inhibe ettiğini desteklemektedir. Bu inhibitör etkileri, mikroorganizmalara ve CFS’lerinin konsantrasyonlarına bağlı olarak değişkendir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Fatma Kalaycı Yüksek ^*
0000-0002-0028-5646
Türkiye

Defne Gümüş
0000-0003-4070-6924
Türkiye

Derya Bayırlı Turan
0000-0002-7505-341X
Türkiye

Yasar Nakipoğlu
0000-0001-7979-7291
Türkiye

Rıza Adaleti
0000-0001-9576-6794
Türkiye

A. Mine Küçüker
0000-0001-8978-219X
Türkiye

Publication Date

December 22, 2021

Submission Date

January 23, 2021

Acceptance Date

April 24, 2021

Published in Issue

Year 2021 Volume: 60 Number: 4

DOI

https://doi.org/10.19161/etd.1037310

IZ

https://izlik.org/JA45YB68PF

Cite

RIS / Bibtex

Vancouver

1.Fatma Kalaycı Yüksek, Defne Gümüş, Derya Bayırlı Turan, Yasar Nakipoğlu, Rıza Adaleti, A. Mine Küçüker. Cell-free supernatants of lactobacilli inhibit methicilin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and carbapenem-resistant Klebsiella strains. EJM. 2021 Dec. 1;60(4):332-9. doi:10.19161/etd.1037310

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