Kök hücreler kanser hücrelerinin mikroçevresindeki sitokin yanıtlarını etkiler: Meme kanseri ve dental pulpa kök hücreleri arasındaki etkileşim

Sayra Dilmaç; Mustafa Gökhan Ertosun; Eda Açıkgöz; Gamze Tanrıöver

doi:10.19161/etd.1036959

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Kök hücreler kanser hücrelerinin mikroçevresindeki sitokin yanıtlarını etkiler: Meme kanseri ve dental pulpa kök hücreleri arasındaki etkileşim

Öz

Amaç: Tümör ve mikroçevresinde bulunan hücreler birbirleri ile yakından ilişkilidir ve sürekli etkileşim halindedirler. Bu hücreler arasındaki karşılıklı etkileşim, tümörün gelişmesi ve metastazında rol oynayan sitokin yanıtlarını şekillendirir. Kök hücreler ile bunların nişleri arasındaki etkileşimlerin de; tümör hücrelerine benzer şekilde karşılıklı olduğu bilinmektedir. Dönüştürücü Büyüme Faktörü Beta1 ve Büyüme/farklılaşma faktörü15, tümör mikroçevresi üzerinde çift yönlü etkileri olan sitokinlerdir. Çalışmamızda, tümör hücreleri ve kök hücrelerin karşılıklı etkileşimlerinin mikroçevrelerindeki sitokin yanıtlarına olan etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Bu çalışmada ko-kültür deneyleri için Dental pulpa kök hücreleri ile MDA-MB-231 meme kanseri hücreleri kullanılmıştır. MDA-MB-231 ve Dental Pulpa Kök Hücreleri hem bireysel hem de birlikte farklı kombinasyonlarla kültüre edildi ve koşullu medyumları toplandı. Toplanan koşullu medyumlarda Dönüştürücü Büyüme Faktörü Beta1 ve Büyüme/farklılaşma faktörü15 sitokin seviyeleri ELISA yöntemi ile değerlendirildi. Bulgular: MDA-MB-231 hücrelerinin koşullu medyumları ile inkübe edilen Dental pulpa kök hücre medyumunda Dönüştürücü Büyüme Faktörü Beta1 ve Büyüme/farklılaşma faktörü15 ekspresyonlarının arttığı ancak, tek tek hücre hatlarından alınan medyumlarda bu sitokin seviyelerinde fark olmadığı görüldü. Seçilen bu sitokin yanıtlarının artması tümör hücrelerinin metastaz potansiyellerini etkileyeceğinden önem içermektedir. Sonuç: Tümör hücreleri ve kök hücreler arasındaki karşılıklı konuşma, tümör mikroçevresindeki sitokinlerin yanıtlarını değiştirir ve tümörün metastaz potansiyelini etkileyebilir. Dolayısıyla çalışmamız tümör mikroçevresinin, tümörün gelecek yanıtlarında önemini vurgulayan bir çalışma olma nitelinde olup bu yönüyle literatüre katkı sağlayacaktır.

Anahtar Kelimeler

Stem cells affect cytokine responses in the microenvironment of tumor cells: Interaction between breast cancer and dental pulp stem cells

Abstract

Aim: Tumor and surrounding microenvironment cells are closely related and interact constantly. The mutual communication between these cells may affect some factors such as cytokines that involved in tumor progression and metastasis. In the interactions between stem cells and their niches; It is known to be reciprocal similar to tumor cells. Transforming Growth Factor Beta1 and Growth Differentiation Factor15 are cytokines which have dual effects on the tumor microenvironment. In our study, we aimed to investigate the effects of interaction of tumor cells and stem cells on cytokine responses in their microenvironment. Materials and Methods: Dental pulp stem cells and MDA-MB-231 cells were used in co-culture experiments in this study. MDA-MB-231 and Dental Pulp Stem Cells were cultured both individually and together in different combinations and their conditional media were collected. Transforming Growth Factor Beta1 and Growth Differentiation Factor15 cytokine levels in conditional media were determined by using ELISA. Results: Expression of Transforming Growth Factor Beta1 and Growth Differentiation Factor15 increased in Dental Pulp Stem Cell medium incubated with conditional media from MDA-MB-231 cells, but there was no difference in these cytokine levels in media taken from individual cell lines. Increasing these selected cytokine responses will affect the metastasis potential of tumor cells. Conclusion: We observed that cross-talks between tumor cells and stem cells alters the responses of cytokines in the tumor microenvironment and affects tumor progression. Therefore, our study emphasizes the importance of the tumor microenvironment in tumor responses, and may contribute to the new perspectives in this respect.

Keywords

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Proje Birimi

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Sayra Dilmaç
0000-0002-1080-6791
Türkiye

Mustafa Gökhan Ertosun
0000-0002-2557-7346
Türkiye

Eda Açıkgöz
0000-0002-6772-3081
Türkiye

Gamze Tanrıöver ^*
0000-0002-8002-5544
Türkiye

Publication Date

December 22, 2021

Submission Date

February 13, 2021

Acceptance Date

February 26, 2021

Published in Issue

Year 2021 Volume: 60 Number: 4

DOI

https://doi.org/10.19161/etd.1036959

IZ

https://izlik.org/JA67FD86PL

Cite

RIS / Bibtex

Vancouver

1.Sayra Dilmaç, Mustafa Gökhan Ertosun, Eda Açıkgöz, Gamze Tanrıöver. Kök hücreler kanser hücrelerinin mikroçevresindeki sitokin yanıtlarını etkiler: Meme kanseri ve dental pulpa kök hücreleri arasındaki etkileşim. EJM. 2021 Dec. 1;60(4):314-23. doi:10.19161/etd.1036959