Year 2018, Volume 57 , Issue 3, Pages 157 - 162 2018-09-14

Significant increase in ZNF304 and decrease in CXCR4 gene expressions may alter anoikis in prostate cancer
ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir

Şule AYLA [1] , Gülperi ÖKTEM [2] , Cüneyd PARLAYAN [3]


Aim: To analyze genes that may increase or inhibit the anoikis mechanism between prostate cancer cell line and prostate normal epithelial cell line and examine the possible role of cancer in cancer development.

Materials and Methods: Human prostate epithelial cell line (RWPE) and prostate cancer cell line (DU145) were acquired from ATCC. Both cell lines were maintanied in RPMI 1640 (Biological Industries) medium. Total RNA were isolated and fragmented. Adapters were ligated to prepare RNA library for whole trasncriptome experiments. Statistics and bioinformatics analysis including mapping, clustering, sequencing were done by using Genomics Workbench v 8. (Qiagen) software.

Results: As we compared the normal prostate ephitalial cells (RWPE) and prosatate cancer cells (DU 145); ZNF304,PYCARD ve NOTCH3 were significantly (p<0.05) up-regulated in DU145 cells, on the other hand, CXCR4, PAK3, SERPINB1 genes were significantly down-regulated.

Conclusion: We found that there are significant differential gene expressions in DU-145 cells which may lead to metastatic state via evasion of anoikis process.

Amaç: Prostat kanser hücre hattı (DU145) ve prostat normal epitel hücre hatları (RWPE) arasında anoikis mekanizmasını arttıracak veya inhibe edebilecek genlerin analizini yapmak ve kanser gelişiminde olası rolünü incelemek.
Gereç ve Yöntem: İnsan prostat epitel hücre hattı (RWPE) ve prostat kanseri hücre hatları (DU-145) Amerikan Tip Kültür Koleksiyonu (ATCC)’den temin edildi. Hücre hatlarının çoğaltılmasında ve sürdürülmesinde RPMI 1640 (Biological Industries) besi ortamı kullanıldı. Transkriptom analizi için RNA izolasyonu yapılarak, kütüphane oluşturuldu, kütüphanenin kantitasyonunun ardından NextSeq500 (illumina) ile sekanslama yapıldı. Dizileme, haritalandırma, bağıl gen ifadeleri ölçümleri gibi biyoinformatik analizler Genomics Workbench v 8 (Qiagen) yazılımı kullanılarak GRCh38 referans sekansı ile yapılmıştır.

Bulgular: RWPE Normal prostat epitel hücre kültürleri ile DU145 prostat kanser hücreleri karşılaştırıldığı zaman DU-145 prostat kanser hücre kültürlerinde, ZNF304, PYCARD ve Notch3 gen expresyonlarında anlamlı bir artış (p<0,05) görülürken, CXCR4, Pak3, SerpınB1 gen ekspresyonlarında anlamlı bir azalma (p<0,05) görülmüştür.
Sonuç: DU145 prostat kanseri hücre hattında anoikis ile ilişkili önemli gen ekpresyonlarında artış ve azalma gözlemledik. Değişime bağlı olarak hücrelerin anoikisden kaçarak metastatik özellik kazanabileceğini düşündük
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Primary Language tr
Subjects Health Care Sciences and Services
Journal Section Research Articles
Authors

Orcid: 0000-0003-2143-5268
Author: Şule AYLA (Primary Author)
Institution: İstanbul Medipol Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER), İstanbul
Country: Turkey


Orcid: 0000-0001-6227-9519
Author: Gülperi ÖKTEM
Institution: Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir
Country: Turkey


Orcid: 0000-0002-6183-9489
Author: Cüneyd PARLAYAN
Institution: İstanbul Medipol Üniversitesi Doğa Bilimleri ve Mühendislik Fakültesi, Biyomedikal Mühendislik Anabilim Dalı, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER), İstanbul, Türkiye
Country: Turkey


Dates

Publication Date : September 14, 2018

Bibtex @research article { etd418162, journal = {Ege Tıp Dergisi}, issn = {1016-9113}, eissn = {2147-6500}, address = {}, publisher = {Ege University}, year = {2018}, volume = {57}, pages = {157 - 162}, doi = {10.19161/etd.418162}, title = {ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir}, key = {cite}, author = {Ayla, Şule and Öktem, Gülperi and Parlayan, Cüneyd} }
APA Ayla, Ş , Öktem, G , Parlayan, C . (2018). ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir . Ege Tıp Dergisi , 57 (3) , 157-162 . DOI: 10.19161/etd.418162
MLA Ayla, Ş , Öktem, G , Parlayan, C . "ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir" . Ege Tıp Dergisi 57 (2018 ): 157-162 <http://egetipdergisi.com.tr/en/pub/issue/39681/418162>
Chicago Ayla, Ş , Öktem, G , Parlayan, C . "ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir". Ege Tıp Dergisi 57 (2018 ): 157-162
RIS TY - JOUR T1 - ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir AU - Şule Ayla , Gülperi Öktem , Cüneyd Parlayan Y1 - 2018 PY - 2018 N1 - doi: 10.19161/etd.418162 DO - 10.19161/etd.418162 T2 - Ege Tıp Dergisi JF - Journal JO - JOR SP - 157 EP - 162 VL - 57 IS - 3 SN - 1016-9113-2147-6500 M3 - doi: 10.19161/etd.418162 UR - https://doi.org/10.19161/etd.418162 Y2 - 2017 ER -
EndNote %0 Ege Tıp Dergisi ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir %A Şule Ayla , Gülperi Öktem , Cüneyd Parlayan %T ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir %D 2018 %J Ege Tıp Dergisi %P 1016-9113-2147-6500 %V 57 %N 3 %R doi: 10.19161/etd.418162 %U 10.19161/etd.418162
ISNAD Ayla, Şule , Öktem, Gülperi , Parlayan, Cüneyd . "ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir". Ege Tıp Dergisi 57 / 3 (September 2018): 157-162 . https://doi.org/10.19161/etd.418162
AMA Ayla Ş , Öktem G , Parlayan C . ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir. ETD. 2018; 57(3): 157-162.
Vancouver Ayla Ş , Öktem G , Parlayan C . ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir. Ege Tıp Dergisi. 2018; 57(3): 157-162.