PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells.

Venhar Gurbuz Can; Tansu Kuşat

doi:10.19161/etd.1623604

EN TR

PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells.

Öz

Aim: Colon cancer is the second most commonly diagnosed cancer among females and the third most frequently diagnosed cancer among males worldwide. Epigenetics is described as alterations in gene expression without changes in nucleotide sequence. Epigenetic mechanisms can modulate the activation or inactivation of cancer-associated genes. The aim of our study was to demonstrate the epigenetic effect of PARP1, DNMT1, and UHRF1 genes in oxaliplatin-treatment colon cancer cell line. Materials and Methods: HT-29 cells were cultured in suitable medium. Then, when the cells were grown up to a certain number, oxaliplatin was applied for 24, 48, and 72 hours at doses of 2.5, 5, 10, 25, 50, 100, and 200 µM to the HT-29 cells, and Cell viability was detected by XTT test. Following the completion of the XTT test, PARP1, DNMT1, and UHRF1 gene expression levels were analyzed by the real-time PCR method. Results: Our results revealed that Oxaliplatin significantly suppressed the expression of UHRF1 and DNMT1 in colon cancer cells, whereas it significantly stimulated the expression of PARP1. Conclusion: In conclusion, the data obtained indicates that the UHRF1, PARP1 and DNMT1 genes may function as modulators of the epigenome and that DNMT1 and UHRF1 interact negatively with PARP1. This study has demonstrated the potential of the UHRF1, PARP1 and DNMT1 genes as targets for colon cancer treatment and as candidate biomarkers.

Anahtar Kelimeler

Oxaliplatin uygulanan kolon kanseri hücrelerinde PARP1, DNMT1 ve UHRF1 üzerinden epigenetik olayları düzenler

Öz

Amaç: Kolon kanseri dünya genelinde kadınlarda en sık görülen ikinci, erkeklerde ise en sık görülen üçüncü kanser türüdür. Epigenetik, nükleotid diziliminde değişiklik olmaksızın gen ifadesinde meydana gelen değişiklikler olarak tanımlanmaktadır. Kanserle ilişkili genlerin aktivasyonu veya inaktivasyonu epigenetik mekanizmalarla modüle edilebilmektedir. Çalışmamızın amacı, oksaliplatin ile tedavi edilen kolon kanseri hücre hattında PARP1, DNMT1 ve UHRF1 genlerinin epigenetik etkisini gösterebilmektir. Gereç ve Yöntem: HT-29 hücreleri uygun besiyeri ortamında kültüre edilmiş, daha sonra, belirli bir sayıya kadar büyütüldüğünde, hücrelere 2.5, 5, 10, 25, 50, 100, 200 µM dozlarında ve 24, 48 ve 72 saat boyunca oksaliplatin uygulanmıştır. Uygulama sonrasında, hücre canlılığı XTT yöntemi ile tespit edilmiştir. XTT yönteminin tamamlanmasını takiben, PARP1, DNMT1 ve UHRF1 genlerinin ekspresyon seviyeleri gerçek zamanlı PCR yöntemi ile analiz edilmiştir. Bulgular: Oxaliplatinin kolon kanseri hücrelerinde UHRF1 ve DNMT1 ekspresyonunu önemli ölçüde baskıladığını, PARP1 ekspresyonunu ise önemli ölçüde uyardığını ortaya koymuştur. Sonuç: Elde ettiğimiz veriler ışığında UHRF1, PARP1ve DNMT1 genlerinin epigenomun bir modülatörü olarak işlev görebileceğini ve DNMT1 ve UHRF1 genlerinin PARP1geni ile negatif korelasyon gösterdiğini tespit ettik. Bu çalışma, UHRF1, PARP1 ve DNMT1'in kolon kanseri tedavileri için potansiyel hedef ve aday biyobelirteçler olarak belirlenebileceğini göstermiştir.

Anahtar Kelimeler

Destekleyen Kurum

yok

Proje Numarası

yok

Etik Beyan

Hücre hattı çalışması olduğu için Etik onay alınmamıştır

Teşekkür

yok

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Kanser Genetiği, Kanser Tanısı, Kemoterapi, Moleküler Hedefler, Öngörücü ve Prognostik Belirteçler

Bölüm

Araştırma Makalesi

Yazarlar

Venhar Gurbuz Can ^*
0000-0002-9777-5173
Türkiye

Tansu Kuşat
0000-0001-7237-1562
Türkiye

Yayımlanma Tarihi

8 Aralık 2025

Gönderilme Tarihi

20 Ocak 2025

Kabul Tarihi

6 Temmuz 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 64 Sayı: 4

DOI

https://doi.org/10.19161/etd.1623604

IZ

https://izlik.org/JA39TN59FK

Kaynak Göster

RIS / Bibtex

APA

Gurbuz Can, V., & Kuşat, T. (2025). PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells. Ege Tıp Dergisi, 64(4), 612-620. https://doi.org/10.19161/etd.1623604

AMA

1.Gurbuz Can V, Kuşat T. PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells. ETD. 2025;64(4):612-620. doi:10.19161/etd.1623604

Chicago

Gurbuz Can, Venhar, ve Tansu Kuşat. 2025. “PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells”. Ege Tıp Dergisi 64 (4): 612-20. https://doi.org/10.19161/etd.1623604.

EndNote

Gurbuz Can V, Kuşat T (01 Aralık 2025) PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells. Ege Tıp Dergisi 64 4 612–620.

IEEE

[1]V. Gurbuz Can ve T. Kuşat, “PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells”., ETD, c. 64, sy 4, ss. 612–620, Ara. 2025, doi: 10.19161/etd.1623604.

ISNAD

Gurbuz Can, Venhar - Kuşat, Tansu. “PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells”. Ege Tıp Dergisi 64/4 (01 Aralık 2025): 612-620. https://doi.org/10.19161/etd.1623604.

JAMA

1.Gurbuz Can V, Kuşat T. PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells. ETD. 2025;64:612–620.

MLA

Gurbuz Can, Venhar, ve Tansu Kuşat. “PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells”. Ege Tıp Dergisi, c. 64, sy 4, Aralık 2025, ss. 612-20, doi:10.19161/etd.1623604.

Vancouver

1.Venhar Gurbuz Can, Tansu Kuşat. PARP1 regulates epigenetic processes through DNMT1 and UHRF1 in oxaliplatin-treated colon cancer cells. ETD. 01 Aralık 2025;64(4):612-20. doi:10.19161/etd.1623604