Farklı implant materyalleri üzerinde osteoblasta farklılaştırılmış kemik iliği stromal kök hücrelerinin (KİSKH) kemik tamirindeki rolü

Cilt: 51 Sayı: 1 1 Mart 2012
  • Özdal Kurt F
  • Vatansever H S
  • Tuğlu İ
  • Deliloğlu Gürhan S İ
Ege Tıp Dergisi Kapak
Ege Tıp Dergisi
PDF İndir
EN TR

The bone repair role of bone marrow stromal cells (BMSC) differentiated to osteoblast on the different implant materials

Abstract

Introduction: It has been thought that tissue engineering could be helpful in the wound healing of the bone by the bone marrow stromal cells (BMSC) on the biomaterials. In this study, the effect of differentiated BMSC on the different biomaterials for in vivo wound healing of experimental bone defect by the implantation will be investigated. Materials and Methods: Bone marrow stromal cells from mature male rat tibia were cultured on the three different (Cellulose Type I, Cellulose type II, Gelatine) biomaterials with culture medium including α-MEM, 10% foetal bovine serum, penicillin, streptomycin, amphotericin and gentamycine. Nonadhesive cells were removed by medium change and attach cells in the culture were incubated by osteoblastic medium including ascorbic acid, dexamethasone and β-glycerophosphate. The differentiated cells were determined by osteoblastic markers such as osteonectin and osteocalcin and were analyzed by vonkossa and alkalen phosphates for their mineralization and bone formation capacity. Their healing capacity was investigated by the histologic and morphometric methods. Results: The capacity of adhesion, proliferation and differentiation of bone marrow stromal cells was positively enhanced by these biomaterials and implantation of these biomaterials with differentiated cells produced better healing on the experimental bone defect. Conclusion: Understanding of the positive interactions between used biomaterials and cells will be helpful for the importance of tissue engineering on the clinical treatment which will promise a better quality of life for patients in the future.

Keywords

Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

-

Yazarlar

Özdal Kurt F

Vatansever H S

Tuğlu İ

Deliloğlu Gürhan S İ

Yayımlanma Tarihi

1 Mart 2012

Gönderilme Tarihi

1 Mart 2012

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2012 Cilt: 51 Sayı: 1

IZ

https://izlik.org/JA98FW52GE

Kaynak Göster

RIS / Bibtex
Vancouver
1.Özdal Kurt F, Vatansever H S, Tuğlu İ, Deliloğlu Gürhan S İ. Farklı implant materyalleri üzerinde osteoblasta farklılaştırılmış kemik iliği stromal kök hücrelerinin (KİSKH) kemik tamirindeki rolü. ETD [Internet]. 01 Mart 2012;51(1):15-20. Erişim adresi: https://izlik.org/JA98FW52GE

Ege Tıp Dergisi, makalelerin Atıf-Gayri Ticari-Aynı Lisansla Paylaş 4.0 Uluslararası (CC BY-NC-SA 4.0) lisansına uygun bir şekilde paylaşılmasına izin verir.