Laboratuvar tasarımı bir HBV DNA kantitasyon protokolü rutin kullanıma uygun olabilir mi? – Ege Üniversitesi Klinik Viroloji Laboratuvarı deneyimi

Cilt: 54 Sayı: 4 1 Aralık 2015
  • Ajda Turhan
  • İmre Altuğlu
  • Selda Erensoy
  • Rüçhan Sertöz
Ege Tıp Dergisi Kapak
Ege Tıp Dergisi
PDF İndir
EN TR

Would a laboratory designed HBV DNA quantification protocol be appropriate for routine use? – Ege University Clinical Virology Laboratory's experience

Abstract

Aim: Hepatitis B virus (HBV) DNA quantification is an important indicator during the follow-up of chronic hepatitis B infection, which has a major public health impact in Turkey due to its hepatocellular carcinoma and fatal liver damage end-points. There are many different methods and systems used in clinical virology laboratories for HBV DNA quantification. The aim of this study was to test whether a laboratory designed real time assay protocole would be compatible with ABI Prism 7500 (PE Biosystems) in Ege University Medical School's Virology/Molecular Biology laboratory and to compare it with the routinely used COBAS AmpliPrep-COBAS TaqMan 48 (CAP-CTM, Roche, Branchburg, NJ) HBV DNA test. Materials and Methods: A real time test based on Taqman technology to detect HBV DNA was designed in the Virology/Molecular Biology Laboratory of Ege University Medical School's Medical Microbiology Department and it was evaluated. 332 samples sent to the laboratory for HBV DNA quantification were included in the study and were compared with the COBAS AmpliPrep-COBAS TaqMan 48 HBV DNA test routinely used in the laboratory. Results: HBV DNA results of 176 out of 332 samples were in the dynamic range of the protocol. The quantitative results of 176 samples in dynamic range were concordant. The results of 106 samples were negative according to both systems. Conclusion: The laboratory designed HBV DNA quantification protocole can be used in the clinical virology laboratory for the routine diagnosis and follow-up of HBV infected patients and it is a suitable and cheap method enabling a wide dynamic range.

Keywords

Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

-

Yazarlar

Ajda Turhan

İmre Altuğlu

Selda Erensoy

Rüçhan Sertöz

Yayımlanma Tarihi

1 Aralık 2015

Gönderilme Tarihi

1 Aralık 2015

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2015 Cilt: 54 Sayı: 4

DOI

https://doi.org/10.19161/etd.344153

IZ

https://izlik.org/JA93TC55PN

Kaynak Göster

RIS / Bibtex
Vancouver
1.Ajda Turhan, İmre Altuğlu, Selda Erensoy, Rüçhan Sertöz. Laboratuvar tasarımı bir HBV DNA kantitasyon protokolü rutin kullanıma uygun olabilir mi? – Ege Üniversitesi Klinik Viroloji Laboratuvarı deneyimi. ETD. 01 Aralık 2015;54(4):159-62. doi:10.19161/etd.344153

Ege Tıp Dergisi, makalelerin Atıf-Gayri Ticari-Aynı Lisansla Paylaş 4.0 Uluslararası (CC BY-NC-SA 4.0) lisansına uygun bir şekilde paylaşılmasına izin verir.