Association of Several Innate Immune Response Gene Polymorphisms with COVID-19 in Turkish Population

Mustafa Soyöz; Zeynep Altın; Aslı Eldem; Alper Togay; Hatice İlayhan Karahan Çöven; Tuba Oz; Melek Pehlivan; Tülay Kılıçaslan Ayna; İbrahim Pirim

doi:10.19161/etd.1351621

EN TR

Association of Several Innate Immune Response Gene Polymorphisms with COVID-19 in Turkish Population

Abstract

Aim: The coronavirus disease 2019 (COVID-19) was caused by severe acute respiratory syndrome 2 virus (SARS-CoV-2), has spread globally. Gender and age have been established as risk factors for severe COVID-19. However, these factors do not fully explain the effects on disease risk. According to researchers, single nucleotide polymorphisms (SNPs) on multiple genes could affect the severity of COVID-19. The progression of viral diseases depends on the characteristics of the patient's innate immunity. The effectiveness of the innate immune system depends on the patient's genetic factors, including SNPs in the TLR, CCR5, and RIG-I genes. In this study, we researched the association of allele and genotype frequency in SNPs of COVID-19 patients with age and gender. Materials and Methods: In our study, 200 patients with moderate COVID-19 were included. Single nucleotide polymorphisms (SNP) of TLR3 (rs3775291, rs3775290, rs5743305), TLR7 (rs179008), TLR8 (rs3764880), RIG-I (rs12006123), and CCR5 (rs1799987) were studied. SNPs were determined by restriction fragment length polymerase chain reaction (RFLP-PCR) methods. Results: In the COVID-19 patients, we examined the patients were evaluated in terms of allele and genotype frequencies and the association between some parameters like age, and gender. In our results, TLR3 rs5743305 AA genotype frequency (p=0.03) and TLR7 rs179008 AA genotype frequency (p=0.03) were found to be significant in terms of age and gender. Conclusions: These SNP data is assessed against disease risk to plan personalized pharmacological therapy for COVID-19 patients.The findings from this study will be useful for genome-wide association studies (GWAS).

Keywords

Türk Popülasyonunda Çeşitli Doğuştan Bağışıklık Tepkisi Gen Polimorfizmlerinin COVID-19 ile İlişkisi

Öz

Amaç: Coronavirüs hastalığı 2019 (COVID-19), şiddetli akut solunum sendromu 2 virüsünün (SARS-CoV-2) neden olduğu ve küresel olarak yayıldığı bir hastalıktır. Cinsiyet ve yaş, ciddi COVİD-19 için risk faktörleri olarak belirlenmiştir. Ancak bu faktörler hastalık riski üzerindeki etkileri tam olarak açıklamamaktadır. Araştırmacılara göre, birden fazla gendeki tek nükleotid polimorfizmleri (SNP'ler), COVID-19'un şiddetini etkileyebilir. Viral hastalıkların ilerlemesi hastanın doğuştan gelen bağışıklığının özelliklerine bağlıdır. Doğuştan gelen bağışıklık sisteminin etkinliği, TLR, CCR5 ve RIG-I genlerindeki SNP'ler dahil olmak üzere hastanın genetik faktörlerine bağlıdır. Bu çalışmada, COVID-19 hastalarının SNP'lerindeki alel ve genotip sıklığının yaş ve cinsiyet ile ilişkisini araştırdık. Gereç ve Yöntem: Çalışmamıza orta şiddette COVİD-19 hastası 200 hasta dahil edildi. TLR3 (rs3775291, rs3775290, rs5743305), TLR7 (rs179008), TLR8 (rs3764880), RIG-I (rs12006123) ve CCR5'in (rs1799987) tek nükleotid polimorfizmleri (SNP) incelenmiştir. SNP'ler, kısıtlama fragmanı uzunluğu polimeraz zincir reaksiyonu (RFLP-PCR) yöntemleriyle belirlendi. Bulgular: COVID-19 hastalarında allel ve genotip frekansları ile yaş, cinsiyet gibi bazı parametreler arasındaki ilişki açısından değerlendirilen hastaları inceledik. Sonuçlarımızda TLR3 rs5743305 AA genotip frekansı (p=0,03) ve TLR7 rs179008 AA genotip frekansı (p=0,03) yaş ve cinsiyet açısından anlamlı bulundu. Sonuç: Bu SNP verileri, COVID-19 hastaları için kişiselleştirilmiş farmakolojik tedaviyi planlamak amacıyla hastalık riskine karşı değerlendirilebilir. Bu çalışmadan elde edilecek bulgular genom çapında ilişkilendirme çalışmaları (GWAS) için faydalı olacaktır.

Anahtar Kelimeler

Supporting Institution

İKÇÜ BAP

Project Number

2020-COV-TIPF-0002

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Details

Primary Language

English

Subjects

Basic Immunology

Journal Section

Research Article

Authors

Mustafa Soyöz ^*
0000-0001-5159-6463
Türkiye

Zeynep Altın
0000-0003-3682-1479
Türkiye

Aslı Eldem
0000-0003-3510-6748
Türkiye

Alper Togay
0000-0002-5256-0064
Türkiye

Hatice İlayhan Karahan Çöven
0000-0002-3371-7345
Türkiye

Tuba Oz
0000-0003-4366-8927
Poland

Melek Pehlivan
0000-0001-8755-4812
Türkiye

Tülay Kılıçaslan Ayna
0000-0001-7993-978X
Türkiye

İbrahim Pirim
0000-0001-8485-3286
Türkiye

Publication Date

June 10, 2024

Submission Date

September 7, 2023

Acceptance Date

November 27, 2023

Published in Issue

Year 1970 Volume: 63 Number: 2

DOI

https://doi.org/10.19161/etd.1351621

IZ

https://izlik.org/JA47FR77HA

Cite

RIS / Bibtex

Vancouver

1.Mustafa Soyöz, Zeynep Altın, Aslı Eldem, Alper Togay, Hatice İlayhan Karahan Çöven, Tuba Oz, Melek Pehlivan, Tülay Kılıçaslan Ayna, İbrahim Pirim. Association of Several Innate Immune Response Gene Polymorphisms with COVID-19 in Turkish Population. EJM. 2024 Jun. 1;63(2):230-9. doi:10.19161/etd.1351621