Protective effect of peroral capsaicin administration in ethanol-induced gastric ulcer model in rats.

Mete Keçeci; Meryem Akpolat Ferah; Habib Khoshvaghti; Osman Cengil

doi:10.19161/etd.1604019

EN TR

Protective effect of peroral capsaicin administration in ethanol-induced gastric ulcer model in rats.

Abstract

Aim: Capsaicin is a compound that possesses antioxidants and anti-inflammatory properties, and it is found in almost all bitter fruits. The objective of our study was to investigate the protective effect of capsaicin in an ethanolic ulcer model. Materials and Methods: The male Wistar albino rats were divided into four groups, comprising a control group and three experimental groups (n=8). The control group (C) administered a single dose of distilled water subcutaneously. The Capsaicin (CAP) group was administered a single dose of 1 mg/kg CAP via gavage. The ulcer group administered a single dose of 1ml absolute alcohol via gavage, 30 minutes following the administration of a single dose of distilled water via gavage. The U+CAP group administered 1ml of absolute alcohol 30 minutes following the administration of 1 mg/kg CAP. Macroscopic and microscopic ulcer scores, as well as mucosal barrier integrity, were evaluated in the gastric tissues that had been removed. The levels of total oxidant status (TOS) and total antioxidant status (TAS) were determined in the tissue samples, and an oxidative stress index (OSI) was calculated. Results: In comparison to the U group, the macroscopic and microscopic mucosal lesions, TOS, OSI levels and IL-1, TNF-α, NFκB and Caspase 3 expressions were found to be decreased in the U+CAP group, while TAS levels were observed to be increased. Conclusion: The results of the study demonstrated that capsaicin protects mucosal integrity through its antioxidant, anti-inflammatory and anti-apoptotic properties in the ethanol-induced gastric ulcer model.

Keywords

Sıçanlarda etanol kaynaklı gastrik ülser modelinde peroral kapsaisin uygulamasının koruyucu etkisi

Öz

Amaç: Kapsaisin, hemen tüm acı biberlerin yapısında bulunan anti-oksidan ve anti-inflamatuar özelliklere sahip bir bileşiktir. Çalışmamızda etanolik ülser modelinde kapsaisin’in koruyucu etkisi araştırılmıştır. Gereç ve Yöntem: Erkek Wistar albino sıçanlar biri kontrol üçü deney grubu olmak üzere dört gruba ayrılmıştır (n=8). Kontrol (C) grubuna tek doz distile su subkutan uygulanmıştır. Kapsaisin (CAP) grubuna tek doz 1 mg/kg CAP gavaj yoluyla uygulanmıştır. Ülser (U) grubuna tek doz distile suyun gavaj yoluyla uygulanmasından 30 dakika sonra tek doz 1 ml mutlak alkol gavaj yoluyla uygulanmıştır. U+CAP grubuna 1 mg/kg CAP uygulanmasından 30 dakika sonra 1 ml mutlak alkol uygulanmıştır. Çıkarılan mide dokularında makroskobik ve mikroskobik ülser skorları ve mukozal bariyer bütünlüğü değerlendirilmiştir. Dokuda total oksidan status (TOS) ve total anti-oksidan status (TAS) seviyeleri ölçülmüş ve oksidatif stres indeksi (OSI) hesaplanmıştır. Bulgular: U grubuyla karşılaştırıldığında, makroskobik ve mikroskobik mukozal lezyonlar, TOS, OSI seviyeleri ve IL-1, TNF-α, NFκB ve Kaspaz 3 ekspresyonlarının U+CAP grubunda azaldığı, ancak TAS seviyesinin arttığı görülmüştür. Sonuç: Çalışma, kapsaisin'in etanol kaynaklı gastrik ülser modelinde anti-oksidan, anti-inflamatuar ve anti-apoptotik özellikleriyle mukozal bütünlüğü koruduğunu göstermiştir.

Anahtar Kelimeler

Supporting Institution

This study was supported by Zonguldak Bülent Ecevit University Scientific Research Projects Coordination (2020-98210206-01).

Project Number

2020-98210206-01

Ethical Statement

The study was conducted in accordance with the guidelines set forth in the Guide for the Care and Use of Laboratory Animals, published by the US Public Health Service. Prior to the commencement of the study, approval was obtained from the Institutional Animal Ethics Committee of Bülent Ecevit University (Zonguldak, Turkey; 2020-06-27/02).

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Details

Primary Language

English

Subjects

Gastroenterology and Hepatology , Pathology

Journal Section

Research Article

Authors

Mete Keçeci ^*
0000-0002-2144-4730
Türkiye

Meryem Akpolat Ferah
0000-0002-3419-1728
Türkiye

Habib Khoshvaghti
0000-0003-0129-6715
Türkiye

Osman Cengil
0000-0002-0702-6751
Türkiye

Publication Date

March 12, 2025

Submission Date

December 19, 2024

Acceptance Date

December 31, 2024

Published in Issue

Year 2025 Volume: 64 Number: 1

DOI

https://doi.org/10.19161/etd.1604019

IZ

https://izlik.org/JA45GP45RK

Cite

RIS / Bibtex

Vancouver

1.Mete Keçeci, Meryem Akpolat Ferah, Habib Khoshvaghti, Osman Cengil. Protective effect of peroral capsaicin administration in ethanol-induced gastric ulcer model in rats. EJM. 2025 Mar. 1;64(1):98-106. doi:10.19161/etd.1604019