Protective effect of peroral capsaicin administration in ethanol-induced gastric ulcer model in rats.

Mete Keçeci; Meryem Akpolat Ferah; Habib Khoshvaghti; Osman Cengil

doi:10.19161/etd.1604019

EN TR

Protective effect of peroral capsaicin administration in ethanol-induced gastric ulcer model in rats.

Abstract

Aim: Capsaicin is a compound that possesses antioxidants and anti-inflammatory properties, and it is found in almost all bitter fruits. The objective of our study was to investigate the protective effect of capsaicin in an ethanolic ulcer model. Materials and Methods: The male Wistar albino rats were divided into four groups, comprising a control group and three experimental groups (n=8). The control group (C) administered a single dose of distilled water subcutaneously. The Capsaicin (CAP) group was administered a single dose of 1 mg/kg CAP via gavage. The ulcer group administered a single dose of 1ml absolute alcohol via gavage, 30 minutes following the administration of a single dose of distilled water via gavage. The U+CAP group administered 1ml of absolute alcohol 30 minutes following the administration of 1 mg/kg CAP. Macroscopic and microscopic ulcer scores, as well as mucosal barrier integrity, were evaluated in the gastric tissues that had been removed. The levels of total oxidant status (TOS) and total antioxidant status (TAS) were determined in the tissue samples, and an oxidative stress index (OSI) was calculated. Results: In comparison to the U group, the macroscopic and microscopic mucosal lesions, TOS, OSI levels and IL-1, TNF-α, NFκB and Caspase 3 expressions were found to be decreased in the U+CAP group, while TAS levels were observed to be increased. Conclusion: The results of the study demonstrated that capsaicin protects mucosal integrity through its antioxidant, anti-inflammatory and anti-apoptotic properties in the ethanol-induced gastric ulcer model.

Keywords

Sıçanlarda etanol kaynaklı gastrik ülser modelinde peroral kapsaisin uygulamasının koruyucu etkisi

Öz

Amaç: Kapsaisin, hemen tüm acı biberlerin yapısında bulunan anti-oksidan ve anti-inflamatuar özelliklere sahip bir bileşiktir. Çalışmamızda etanolik ülser modelinde kapsaisin’in koruyucu etkisi araştırılmıştır. Gereç ve Yöntem: Erkek Wistar albino sıçanlar biri kontrol üçü deney grubu olmak üzere dört gruba ayrılmıştır (n=8). Kontrol (C) grubuna tek doz distile su subkutan uygulanmıştır. Kapsaisin (CAP) grubuna tek doz 1 mg/kg CAP gavaj yoluyla uygulanmıştır. Ülser (U) grubuna tek doz distile suyun gavaj yoluyla uygulanmasından 30 dakika sonra tek doz 1 ml mutlak alkol gavaj yoluyla uygulanmıştır. U+CAP grubuna 1 mg/kg CAP uygulanmasından 30 dakika sonra 1 ml mutlak alkol uygulanmıştır. Çıkarılan mide dokularında makroskobik ve mikroskobik ülser skorları ve mukozal bariyer bütünlüğü değerlendirilmiştir. Dokuda total oksidan status (TOS) ve total anti-oksidan status (TAS) seviyeleri ölçülmüş ve oksidatif stres indeksi (OSI) hesaplanmıştır. Bulgular: U grubuyla karşılaştırıldığında, makroskobik ve mikroskobik mukozal lezyonlar, TOS, OSI seviyeleri ve IL-1, TNF-α, NFκB ve Kaspaz 3 ekspresyonlarının U+CAP grubunda azaldığı, ancak TAS seviyesinin arttığı görülmüştür. Sonuç: Çalışma, kapsaisin'in etanol kaynaklı gastrik ülser modelinde anti-oksidan, anti-inflamatuar ve anti-apoptotik özellikleriyle mukozal bütünlüğü koruduğunu göstermiştir.

Anahtar Kelimeler

Destekleyen Kurum

Zonguldak Bülent Ecevit Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

2020-98210206-01

Etik Beyan

Çalışma, ABD Halk Sağlığı Servisi tarafından yayınlanan Laboratuvar Hayvanlarının Bakımı ve Kullanımı Kılavuzu'nda belirtilen yönergelere uygun olarak yürütüldü. Çalışmaya başlamadan önce Bülent Ecevit Üniversitesi Kurumsal Hayvan Etik Kurulu'ndan (Zonguldak, Türkiye; 2020-06-27/02) onay alındı.

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Gastroenteroloji ve Hepatoloji , Patoloji

Bölüm

Araştırma Makalesi

Yazarlar

Mete Keçeci ^*
0000-0002-2144-4730
Türkiye

Meryem Akpolat Ferah
0000-0002-3419-1728
Türkiye

Habib Khoshvaghti
0000-0003-0129-6715
Türkiye

Osman Cengil
0000-0002-0702-6751
Türkiye

Yayımlanma Tarihi

12 Mart 2025

Gönderilme Tarihi

19 Aralık 2024

Kabul Tarihi

31 Aralık 2024

Yayımlandığı Sayı

Yıl 2025 Cilt: 64 Sayı: 1

DOI

https://doi.org/10.19161/etd.1604019

IZ

https://izlik.org/JA45GP45RK

Kaynak Göster

RIS / Bibtex

Vancouver

1.Mete Keçeci, Meryem Akpolat Ferah, Habib Khoshvaghti, Osman Cengil. Protective effect of peroral capsaicin administration in ethanol-induced gastric ulcer model in rats. ETD. 01 Mart 2025;64(1):98-106. doi:10.19161/etd.1604019