Alfa-pinen rotenonla oluşturulan parkinson hastalığı sıçan modelinde soleus kası ve mitokondriyal DNA’yı korur

Emel Kabartan Çökeli; Berna Tezcan Yavuz; Cansın Şirin; Gulay Hacıoğlu; Selma Cırrık

doi:10.19161/etd.1762616

TR EN

Alfa-pinen rotenonla oluşturulan parkinson hastalığı sıçan modelinde soleus kası ve mitokondriyal DNA’yı korur

Öz

Amaç: Bu çalışma, rotenon ile indüklenen Parkinson hastalığı (PH) sıçan modelinde, alfa-pinen uygulamasının soleus kasındaki histolojik yapı ve mitokondriyal DNA (mtDNA) kopya sayısı üzerindeki etkilerini değerlendirmeyi amaçlamaktadır. Gereç ve Yöntem: Yetişkin erkek Sprague-Dawley sıçanlar, kontrol, rotenon (ROT), alfa-pinen (APN) ve alfa-pinen+rotenon (APN+ROT) gruplarına ayrıldı (n=12/grup). ROT (2 mg/kg) ve APN (50 mg/kg), 28 gün boyunca subkutan uygulandı. Soleus kasının histolojik yapısı Hematoksilen-Eozin (H&E) boyama ile değerlendirildi. mtDNA kopya sayısı, kantitatif polimeraz zincir reaksiyonu ile nükleer DNA’ya oranlanarak hesaplandı. Bulgular: ROT grubunda soleus kasında atrofi, kas lifi dejenerasyonu ve fibrozis görülürken mtDNA kopya sayısında anlamlı azalma meydana geldi (p<0,01). APN tedavisi, histolojik bozulmaları ve mtDNA kaybını önemli ölçüde azalttı (p<0,05). APN+ROT grubunda mtDNA kopya sayısı kontrol seviyesine yaklaştı (p>0,05) ve dokuda belirgin histolojik iyileşmeler gözlendi. Sonuç: Alfa-pinen, rotenon ile indüklenen PH modelinde soleus kasında yapısal ve moleküler düzeyde koruyucu etkiler göstererek, periferik mitokondriyal disfonksiyona karşı potansiyel bir terapötik ajan olarak değerlendirilebilir.

Anahtar Kelimeler

Alpha-pinene protects soleus muscle and mitochondrial DNA in a rotenone-induced parkinson’s disease rat model

Abstract

Aim: This study aims to evaluate the effects of alpha-pinene administration on the histological structure and mitochondrial DNA (mtDNA) copy number in the soleus muscle in a rotenone-induced Parkinson's disease (PD) rat model. Materials and Methods: Adult male Sprague-Dawley rats were divided into control, rotenone (ROT), alpha-pinene (APN), and alpha-pinene + rotenone (APN+ROT) groups (n=12/group). ROT (2 mg/kg) and APN (50 mg/kg) were administered subcutaneously for 28 days. The histological structure of the soleus muscle was assessed using Hematoxylin-Eosin (H&E) staining. The mtDNA copy number was calculated as a ratio to nuclear DNA using quantitative polymerase chain reaction (qPCR). Results: In the ROT group, atrophy, muscle fiber degeneration, and fibrosis were observed in the soleus muscle, along with a significant decrease in mtDNA copy number (p<0.01). APN treatment significantly reduced histological damage and mtDNA loss (p<0.05). In the APN+ROT group, the mtDNA copy number approached control levels (p>0.05), with significant histological improvements observed. Conclusion: Alpha-pinene exhibits protective effects at both structural and molecular levels in the soleus muscle in a rotenone-induced PD model, suggesting its potential as a therapeutic agent against peripheral mitochondrial dysfunction.

Keywords

Ethical Statement

The study protocol received formal approval from the local animal ethics committee of the Giresun University (Ethical Approval No: 2025/6, date: 29.07.2025).

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Details

Primary Language

Turkish

Subjects

Neurology and Neuromuscular Diseases , Medical Physiology (Other)

Journal Section

Research Article

Authors

Emel Kabartan Çökeli ^*
0000-0002-6289-2310
Türkiye

Berna Tezcan Yavuz
0000-0002-7410-9485
Türkiye

Cansın Şirin
0000-0002-4530-701X
Türkiye

Gulay Hacıoğlu
0000-0002-8528-2371
Türkiye

Selma Cırrık
0000-0001-8474-0185
Türkiye

Publication Date

September 8, 2025

Submission Date

August 12, 2025

Acceptance Date

August 18, 2025

Published in Issue

Year 2025 Volume: 64 Number: 3

DOI

https://doi.org/10.19161/etd.1762616

IZ

https://izlik.org/JA29KE66DN

Cite

RIS / Bibtex

Vancouver

1.Emel Kabartan Çökeli, Berna Tezcan Yavuz, Cansın Şirin, Gulay Hacıoğlu, Selma Cırrık. Alfa-pinen rotenonla oluşturulan parkinson hastalığı sıçan modelinde soleus kası ve mitokondriyal DNA’yı korur. EJM. 2025 Sep. 1;64(3):589-97. doi:10.19161/etd.1762616