Immunohistochemical demonstration of leptin expression in the rat stomach

Volume: 48 Number: 1 March 1, 2009
  • Gülle K
  • Uyanıkgil Y
  • Karaöz E
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Immunohistochemical demonstration of leptin expression in the rat stomach

Abstract

Introduction: Leptin is a 16-kDA-protein molecule, expressed by the ob gene. It has first been found in the adipose tissue and several tissues in the body have recently been described to express leptin. Various reports have suggested that leptin can also involve in stomach functions. This study was focused on the expression and distribution of leptin in rat stomach using immunohistochemistry. Material and Methods: The study was conducted on male rats weighing 190–210 g. For light microscopic examination, 5 Wistar albino rats were anaesthetized and perfused transcardiacally with heparinised saline followed by %4 paraformaldehyde in phosphate buffer. Leptin immunohistochemistry was performed on stomach tissues preprocessed for routine histological examination at 5 µm slices. Results: The number of leptin (+) principal cells and parietal cells in the fundus of stomach was significantly higher than the corpus. The comparison of leptin (+) cell number within the corpus revealed no significant difference between the number of principal cells and parietal cells; however statistical analysis within the fundus showed that leptin (+) principal cell number was significantly higher than leptin (+) parietal cell number. Moreover, marked stain was observed in the myenteric plexus. Conclusion: Differing from the available studies, data obtained in the current study show that leptin is expressed in the myenteric plexus controlling the gastrointestinal peristaltism. It has been postulated that leptin may participate synergistically in the short- and/or long-term regulation of alimentary functions.

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Primary Language

Turkish

Subjects

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Journal Section

-

Authors

Gülle K

Uyanıkgil Y

Karaöz E

Publication Date

March 1, 2009

Submission Date

March 1, 2009

Acceptance Date

-

Published in Issue

Year 2009 Volume: 48 Number: 1

IZ

https://izlik.org/JA82WN53MW

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RIS / Bibtex
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1.Gülle K, Uyanıkgil Y, Karaöz E. Sıçan midesinde leptin ekspresyonunun immunohistokimyasal olarak gösterilmesi. EJM [Internet]. 2009 Mar. 1;48(1):1-6. Available from: https://izlik.org/JA82WN53MW

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