Simultaneous detection of respiratory viruses using multiplex PCR method in patients with upper and lower respiratory tract infection

Volume: 54 Number: 1 March 1, 2015
  • Candan Çiçek
  • Ayşe Arslan
  • Eylem Ulaş Saz
  • Hüsnü Pullukçu
  • Gürsel Çok
EN TR

Simultaneous detection of respiratory viruses using multiplex PCR method in patients with upper and lower respiratory tract infection

Abstract

Aim: In this study, we aimed to determine the respiratory viruses in a short time by multiplex PCR method in patients with upper and lower respiratory tract infections. Materials and Methods: In this report, between January 2007 and August 2013, a total of 1708 patients (1220 children, 488 adult; 45,1% female, 54.9% male, age range: 5 days-94 years) prediagnosed as having upper or lower respiratory tract infections were enrolled in the study. Respiratory viruses were investigated by multiplex PCR method in respiratory samples of these patients. Results: In a total of 1708 patients, 620 (36.3%) of them were positive for one or more respiratory viruses and 1088 (63.7%) were negative. In 63 of the patients (3.7%) multiple viruses were detected. Most of the samples (89.5%) containing multiple agents were detected in children. Respiratory specimens were obtained from 387 (22.7%) outpatients and 1321 (77.3%) inpatients. One or more respiratory viruses were detected in 39.2% of pediatric patients, 29.1% of adult patients (p

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Primary Language

Turkish

Subjects

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Journal Section

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Authors

Candan Çiçek

Ayşe Arslan

Eylem Ulaş Saz

Hüsnü Pullukçu

Gürsel Çok

Publication Date

March 1, 2015

Submission Date

March 1, 2015

Acceptance Date

-

Published in Issue

Year 1970 Volume: 54 Number: 1

Vancouver
1.Candan Çiçek, Ayşe Arslan, Eylem Ulaş Saz, Hüsnü Pullukçu, Gürsel Çok. Üst ve alt solunum yolu enfeksiyonu olan hastalarda solunum virüslerinin multipleks PCR yöntemi ile eşzamanlı saptanması. EJM. 2015 Mar. 1;54(1):8-14. doi:10.19161/etd.344100

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