AML ön tanılı olgularda inv(16) CBFBETA-MYH11 inversiyonunun real time RT-PCR ile 5 yıllık sonuçlarının değerlendirilmesi

Aslı TETİK Vardarlı; Burçin TEZCANLI Kaymaz; Vildan BOZOK Çetintaş; Sunde YILMAZ Süslüer; Duygu Aygüneş; Ayşegül Dalmızrak; Çağdaş Aktan; Ali Şahin Küçükaslan; Tuğçe Balcı; Çağla Kayabaşı; Besra Özmen Yelken; Nur Selvi Günel; Çığır Biray Avcı; Buket Kosova; Zuhal Eroğlu; Serap Aksoylar; Nazan Çetingül Can Balkan; Deniz Yılmaz; Yeşim Aydınok; Kaan Kavaklı; Mahmut Töbü; Murat Tombuloğlu; Filiz Büyükkeçeci; Fahri Şahin; Güray Saydam; Cumhur Gündüz

doi:10.19161/etd.344103

EN TR

Evaluation of 5-year results of the inversion of inv(16) CBFBETA-MYH11 with real time RT-PCR in patients prediagnosed as AML

Abstract

Aim: In this study, it was aimed to evaluate the analysis of inv16 blood and bone marrow samples quantitively by reverse transcriptase polymerase chain reaction (RT-PCR) method of 402 patients (322 adult, 80 children) prediagnosed as acute myeloid leukemia (AML), who were admitted to Ege University Faculty of Medicine, Department of Medical Biology between years 2009-2013. Materials and Methods: cDNA's were obtained following the total RNA/mRNA isolation which were taken from patients' blood and bone marrow samples and quantitaion of inv16 were performed by reverse transcriptase RT-PCR method at LightCycler2. Results: Four hundred and two cases were evaluated in terms of inv(16) CBFBETA-MYH11 inversion. As a result of the analysis, a total of 19 patient were determined inv16 positivity includes 12 adult (% 4) and 7 children (9%). Conclusion: Inv16 of quantitative analysis is supported to be an effective method for clinical evaluation of patients with AML, regarding remission of disease and implimentation of the treatment protocols toward achieving remission. More studies with AML patients are required for confirmation of the results.

Keywords

Acute myeloid leukemia, inv16, CBFBETA-MYH11, RT-PCR

Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Aslı TETİK Vardarlı

Burçin TEZCANLI Kaymaz

Vildan BOZOK Çetintaş

Sunde YILMAZ Süslüer

Duygu Aygüneş

Ayşegül Dalmızrak

Çağdaş Aktan

Ali Şahin Küçükaslan

Tuğçe Balcı

Çağla Kayabaşı

Besra Özmen Yelken

Nur Selvi Günel

Çığır Biray Avcı

Buket Kosova

Zuhal Eroğlu

Serap Aksoylar

Nazan Çetingül Can Balkan

Deniz Yılmaz

Yeşim Aydınok

Kaan Kavaklı

Mahmut Töbü

Murat Tombuloğlu

Filiz Büyükkeçeci

Fahri Şahin

Güray Saydam

Cumhur Gündüz

Publication Date

March 1, 2015

Submission Date

March 1, 2015

Acceptance Date

-

Published in Issue

Year 1970 Volume: 54 Number: 1

DOI

https://doi.org/10.19161/etd.344103

IZ

https://izlik.org/JA48YG75KT

Cite

RIS / Bibtex

Vancouver

1.Aslı TETİK Vardarlı, Burçin TEZCANLI Kaymaz, Vildan BOZOK Çetintaş, Sunde YILMAZ Süslüer, Duygu Aygüneş, Ayşegül Dalmızrak, Çağdaş Aktan, Ali Şahin Küçükaslan, Tuğçe Balcı, Çağla Kayabaşı, Besra Özmen Yelken, Nur Selvi Günel, Çığır Biray Avcı, Buket Kosova, Zuhal Eroğlu, Serap Aksoylar, Nazan Çetingül Can Balkan, Deniz Yılmaz, Yeşim Aydınok, Kaan Kavaklı, Mahmut Töbü, Murat Tombuloğlu, Filiz Büyükkeçeci, Fahri Şahin, Güray Saydam, Cumhur Gündüz. AML ön tanılı olgularda inv(16) CBFBETA-MYH11 inversiyonunun real time RT-PCR ile 5 yıllık sonuçlarının değerlendirilmesi. EJM. 2015 Mar. 1;54(1):26-9. doi:10.19161/etd.344103

Evaluation of 5-year results of the inversion of inv(16) CBFBETA-MYH11 with real time RT-PCR in patients prediagnosed as AML

Abstract

Keywords