Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi

Eray Özgün; Gülben Sayılan Özgün

doi:10.19161/etd.610836

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Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi

Öz

Amaç: Bu çalışmanın amacı, aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 protein düzeylerine ve arilesteraz aktivitesine etkisini araştırmaktır.

Gereç ve Yöntem: HepG2 hücreleri kontrol, 0.25 mM aspirin, 0.5 mM aspirin ve 1 mM aspirin olmak üzere 4 gruba ayrıldı ve hücreler 48 saat boyunca sırasıyla 0, 0.25, 0.5 ve 1 mM aspirin ile inkübe edildi. Hücre canlılığı 3-(4,5-Dimetil-2-tiazolil)-2,5-difenil-2H-tetrazolium bromür testi ile ölçüldü. Paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 protein düzeyleri western blot yöntemiyle ölçüldü. Arilesteraz aktivitesi substrat olarak fenilasetat kullanılarak spektrofotometrik olarak ölçüldü.

Bulgular: 0.5 mM ve 1 mM aspirin hücre canlılığında anlamlı azalmaya yol açtı. Aspirin konsantrasyonları paraoksonaz-1 ve paraoksonaz-2 protein düzeylerini anlamlı olarak değiştirmedi. 0.5 ve 1 mM aspirin paraoksonaz-3 protein düzeylerini anlamlı olarak arttırdı. 0.25 mM, 0.5 mM ve 1 mM aspirin, arilesteraz aktivitesini anlamlı olarak arttırdı.

Sonuç: Çalışmamız aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz-1 ve paraoksonaz-2 protein düzeylerini değiştirmediğini, paraoksonaz-3 protein düzeylerini ve arilesteraz aktivitesini arttırdığını gösterdi.

Anahtar Kelimeler

Aspirin,paraoksonaz-1,paraoksonaz-2,paraoksonaz-3,arilesteraz.

References

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Eray Özgün ^*
0000-0002-6744-1519
Türkiye

Gülben Sayılan Özgün
0000-0001-6990-3484
Türkiye

Publication Date

September 20, 2019

Submission Date

October 11, 2018

Acceptance Date

November 29, 2018

Published in Issue

Year 2019 Volume: 58 Number: 3

DOI

https://doi.org/10.19161/etd.610836

IZ

https://izlik.org/JA49JJ72FG

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1.Eray Özgün, Gülben Sayılan Özgün. Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi. EJM. 2019 Sep. 1;58(3):295-302. doi:10.19161/etd.610836

Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi

Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi

Öz

Anahtar Kelimeler

Effect of aspirin on protein levels of paraoxonase enzymes and arylesterase activity in human-derived hepatoma cells

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