Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation

Serap Uslu; Gülperi Öktem; Fatih Oltulu; Kenan Demir; Arzu İrban; Gülçin Başdemir; Ümit İnce; Ayşegül Uysal

doi:10.19161/etd.697866

TR EN

Maternal uterin arter ligasyon modeli uygulanan fetal sıçanlarda kondrogenez ve osteogenezin Wnt/β-Catenin, S100 immünoekspresyon ve histomorfometri ile değerlendirilmesi

Öz

Amaç: Bu çalışmanın amacı, sıçanlarda maternal uterin arter ligasyonuna bağlı olarak gelişen intrauterine büyüme geriliğinde kondrogenez ve osteogenezin Wnt/β-catenin ve S100 ekspresyonlarının immünohistokimyasal ve histomorfometrik olarak değerlendirilmesi.

Gereç ve Yöntem: Gebe sıçanlar 3 gruba ayrıldı (n=5). Kontrol grubuna cerrahi veya anestezi uygulanmadı. Deney grubunda, 18. gebelik gününde bilateral maternal uterin arterlere ligasyon yapıldı. Sham grubuna uterin arter ligasyonu dışındaki tüm cerrahi işlem uygulandı. Fetuslar gebeliğin 20. gününde alındı, sol proksimal tibiadan büyüme plağı ve zonların kalınlıkları, trabeküler sayı ve kalınlıkları, kortikal kalınlıklar histomorfometrik olarak değerlendirildi. Büyüme plağında β-catenin ve S100 ekspresyonları immunohistokimyasal olarak değerlendirildi.

Bulgular: Deney grubunda büyüme plağı (p<0.01), proliferasyon zonu (p<0.05) ve dejenerasyon zonun kalınlıkları (p<0.01) belirgin olarak daha ince bulundu. Hipertrofik zonların kalınlığı daha az olarak ölçüldü (p>0.05). Aynı zamanda daha düşük sayıda trabekülar yapı (p<0.01) ve daha ince trabeküler kalınlık (p<0.05) bulundu. Deney grubunda β-catenin ekspresyonu daha düşük iken S100 ekspresyonunda artış saptandı.

Sonuç: Maternal uterin arter ligasyonuna bağlı fetal sıçan intrauterine büyüme geriliği modelinde, Wnt/β-catenin sinyal yolağındaki azalmaya bağlı olarak dejenere kemik yapısı ve büyüme plağında kısalmaya yol açmaktadır.

Anahtar Kelimeler

İntrauterine büyüme geriliği,uterin arter ligasyonu,büyüme plağı,β-catenin,S100,histomorfometri

Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation

Abstract

Aim: The aim of this study is to investigate the effects of intrauterine growth retardation depending on maternal uterine artery ligation, Wnt/β-catenin and S100 expression immunohistochemistry and histomorphometrically on chondrogenesis and osteogenesis of fetal rats.

Materials and Methods: Maternal rats were randomly divided into 3 groups (n=5). No surgery or anesthesia were applied in control group. Bilaterally the maternal uterine arteries were ligated on gestational day 18 in experimental group. Although all surgical procedures were performed in sham group, the uterine artery ligation were not made. Fetuses were taken on gestational day 20, thicknesses of growth plate and zones, trabecular number and thickness and cortical thickness were evaluated with the histomorphometrically in samples from left proximal tibia. The expressions of β-catenin and S100 immunohistochemically were evaluated in the growth plate.

Results: Thicknesses of growth plate (p<0.01), proliferation zone (p<0.05) and degeneration zone (p<0.01) were measured significantly thinner in experimental group than the others and thicknesses of hypertrophic zones were lesser than the control and sham group, but the results were not statistically significant (p>0.05). Also trabecular numbers were lower (p<0.01) and trabecular thickness were also thinner (p<0.05) in experimental group. Expression of β-catenin was declined and S100 expression was increased in experimental group.

Conclusion: We conclude that maternal uterine artery ligation, leads to shortness of growth plate and degenerated bone architecture because of Wnt/β-catenin signaling pathway.

Keywords

Intrauterine growth retardation,uterine artery ligation,growth plate,β-catenin,S100,histomorphometry

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Serap Uslu ^*
0000-0002-6613-527X
Türkiye

Gülperi Öktem
0000-0001-6227-9519
Türkiye

Fatih Oltulu
0000-0001-6475-642X
Türkiye

Kenan Demir
0000-0003-2864-6041
Türkiye

Arzu İrban
0000-0002-4904-0658
Türkiye

Gülçin Başdemir
0000-0002-2104-8957
Türkiye

Ümit İnce
0000-0002-6113-661X
Türkiye

Ayşegül Uysal
0000-0002-9919-2254
Türkiye

Publication Date

March 13, 2020

Submission Date

December 28, 2018

Acceptance Date

April 29, 2019

Published in Issue

Year 2020 Volume: 59 Number: 1

DOI

https://doi.org/10.19161/etd.697866

IZ

https://izlik.org/JA59MM73YC

Cite

RIS / Bibtex

Vancouver

1.Serap Uslu, Gülperi Öktem, Fatih Oltulu, Kenan Demir, Arzu İrban, Gülçin Başdemir, Ümit İnce, Ayşegül Uysal. Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation. EJM. 2020 Mar. 1;59(1):39-46. doi:10.19161/etd.697866