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Alfa-pinen rotenonla oluşturulan parkinson hastalığı sıçan modelinde soleus kası ve mitokondriyal DNA’yı korur

Year 2025, Volume: 64 Issue: 3, 589 - 597, 08.09.2025

Emel Kabartan Çökeli , Berna Tezcan Yavuz , Cansın Şirin , Gulay Hacıoğlu , Selma Cırrık

https://doi.org/10.19161/etd.1762616

Abstract

Amaç: Bu çalışma, rotenon ile indüklenen Parkinson hastalığı (PH) sıçan modelinde, alfa-pinen uygulamasının soleus kasındaki histolojik yapı ve mitokondriyal DNA (mtDNA) kopya sayısı üzerindeki etkilerini değerlendirmeyi amaçlamaktadır.
Gereç ve Yöntem: Yetişkin erkek Sprague-Dawley sıçanlar, kontrol, rotenon (ROT), alfa-pinen (APN) ve alfa-pinen+rotenon (APN+ROT) gruplarına ayrıldı (n=12/grup). ROT (2 mg/kg) ve APN (50 mg/kg), 28 gün boyunca subkutan uygulandı. Soleus kasının histolojik yapısı Hematoksilen-Eozin (H&E) boyama ile değerlendirildi. mtDNA kopya sayısı, kantitatif polimeraz zincir reaksiyonu ile nükleer DNA’ya oranlanarak hesaplandı.
Bulgular: ROT grubunda soleus kasında atrofi, kas lifi dejenerasyonu ve fibrozis görülürken mtDNA kopya sayısında anlamlı azalma meydana geldi (p<0,01). APN tedavisi, histolojik bozulmaları ve mtDNA kaybını önemli ölçüde azalttı (p<0,05). APN+ROT grubunda mtDNA kopya sayısı kontrol seviyesine yaklaştı (p>0,05) ve dokuda belirgin histolojik iyileşmeler gözlendi.
Sonuç: Alfa-pinen, rotenon ile indüklenen PH modelinde soleus kasında yapısal ve moleküler düzeyde koruyucu etkiler göstererek, periferik mitokondriyal disfonksiyona karşı potansiyel bir terapötik ajan olarak değerlendirilebilir.

Keywords

Alfa-pinen Rotenon Parkinson modeli Soleus kası mtDNA Histoloji

References

  • Chen C, Turnbull DM, Reeve AK. Mitochondrial Dysfunction in Parkinson’s Disease—Cause or Consequence? Biology 2019, Vol 8, Page 38.
  • Sherer TB, Kim JH, Betarbet R, Greenamyre JT. Subcutaneous Rotenone Exposure Causes Highly Selective Dopaminergic Degeneration and α-Synuclein Aggregation. Exp Neurol. 2003 Jan 1;179(1):9–16.
  • Panov A, Dikalov S, Shalbuyeva N, Taylor G, Sherer T, Greenamyre JT. Rotenone Model of Parkinson Disease. Journal of Biological Chemistry. 2005 Dec;280(51):42026–35.
  • Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, et al. Mitochondrial Complex I Inhibitor Rotenone Induces Apoptosis through Enhancing Mitochondrial Reactive Oxygen Species Production. Journal of Biological Chemistry. 2003 Mar;278(10):8516–25.
  • Aydınoğlu AT, Aksu Mengeş E, Balcı Hayta B. Serbest Mitokondriyal Dna nın Sistemik Dolaşımdaki Rolü Ve Hastalıkların Patogenezine Etkisi. Acibadem Universitesi Saglik Bilimleri Dergisi. 2020 Jan 1.
  • Musci R V., Fuqua JD, Peelor FF, Nguyen HVM, Richardson A, Choi S, et al. Age-induced changes in skeletal muscle mitochondrial DNA synthesis, quantity, and quality in genetically unique rats. Geroscience. 2025 Feb 1; 47(1):851–62.
  • Miller DM, Singh IN, Wang JA, Hall ED. Nrf2-ARE activator carnosic acid decreases mitochondrial dysfunction, oxidative damage and neuronal cytoskeletal degradation following traumatic brain injury in mice. Exp Neurol. 2015 Feb 1; 264:103–10.
  • Carling PJ, Cree LM, Chinnery PF. The implications of mitochondrial DNA copy number regulation during embryogenesis. Mitochondrion. 2011 Sep;11(5):686–92.
  • Filograna R, Mennuni M, Alsina D, Larsson NG. Mitochondrial DNA copy number in human disease: the more the better? FEBS Lett. 2021 Apr 1; 595(8):976–1002.
  • Hood DA, Memme JM, Oliveira AN, Triolo M. Maintenance of Skeletal Muscle Mitochondria in Health, Exercise, and Aging. Annu Rev Physiol. 2019 Feb 10;81(Volume 81, 2019):19–41.
  • Venkatesan D, Iyer M, Narayanasamy A, Gopalakrishnan AV, Vellingiri B. Plausible Role of Mitochondrial DNA Copy Number in Neurodegeneration—a Need for Therapeutic Approach in Parkinson’s Disease (PD). Molecular Neurobiology 2023 60:12. 2023 Jul 31;60(12):6992–7008.
  • Rahmani H, Moloudi MR, Hashemi P, Hassanzadeh K, Izadpanah E. Alpha-Pinene Alleviates Motor Activity in Animal Model of Huntington’s Disease via Enhancing Antioxidant Capacity. Neurochem Res. 2023 Jun 1;48(6):1775–82.
  • Cirrik S, Hacioglu G, Kabartan E, Yavuz BT, Tomruk CS. Can Alpha-Pinene Prevent Methotrexate-Induced Cardiac and Hepatic Damage? Physiol Res. 2024 ;73(4):621.
  • Hajizadeh Moghaddam A, Malekzadeh Estalkhi F, Khanjani Jelodar S, Ahmed Hasan T, Farhadi-Pahnedari S, Karimian M. Neuroprotective effects of alpha-pinene against behavioral deficits in ketamine-induced mice model of schizophrenia: Focusing on oxidative stress status. IBRO Neurosci Rep. 2024 Jun 1; 16:182–9.
  • Yeh Y, Liu T, Wang L, Lee H, Ting C, Lee W, et al. A standardized extract of Ginkgo biloba suppresses doxorubicin‐induced oxidative stress and p53‐mediated mitochondrial apoptosis in rat testes. Br J Pharmacol. 2009 Jan 24;156(1):48–61.
  • Schmidt S, Messner CJ, Gaiser C, Hämmerli C, Suter-Dick L. Methotrexate-Induced Liver Injury Is Associated with Oxidative Stress, Impaired Mitochondrial Respiration, and Endoplasmic Reticulum Stress In Vitro. Int J Mol Sci. 2022 Dec 1; 23(23):15116.
  • Zuzarte M, Sousa C, Alves-Silva J, Salgueiro L. Plant Monoterpenes and Essential Oils as Potential Anti-Ageing Agents: Insights from Preclinical Data. Biomedicines 2024, Vol 12, Page 365. 2024 Feb 4;12(2):365.
  • Tezcan Yavuz B, Kabartan Cokeli E, Sirin Tomruk C, Hacioglu G, Cirrik S, Tomruk C. Alpha Pinene Affects Intestinal Permeability and Protects the Gastrointestinal System Against Rotenone Toxicity via the Keap1/Nrf2 Pathway in Rats. Neurotoxicity Research 2025 43:4. 2025 Jul 28;43(4):1–12.
  • Kabartan E, Cirrik S, Hacioglu G, Tezcan Yavuz B, Telkoparan-Akillilar P, Sirin Tomruk C. Therapeutic potential of alpha-pinene in methotrexate-induced testicular toxicity: focus on apoptosis, oxidative stress, and inflammatory pathways. Naunyn Schmiedebergs Arch Pharmacol. 2025 Jul 9;1–11.
  • Zhang ZN, Zhang JS, Xiang J, Yu ZH, Zhang W, Cai M, et al. Subcutaneous rotenone rat model of Parkinson’s disease: Dose exploration study. Brain Res. 2017 Jan 15; 1655:104–13.
  • Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov A V., Greenamyre JT. Chronic systemic pesticide exposure reproduces features of Parkinson’s disease. Nature Neuroscience 2000 3:12. 2000;3(12):1301–6.
  • Siddiqui MA, Ahmad J, Farshori NN, Saquib Q, Jahan S, Kashyap MP, et al. Rotenone-induced oxidative stress and apoptosis in human liver HepG2 cells. Mol Cell Biochem. 2013 Dec 21;384(1–2):59–69.
  • Zhou Q, Chen B, Wang X, Wu L, Yang Y, Cheng X, et al. Sulforaphane protects against rotenone-induced neurotoxicity in vivo: Involvement of the mTOR, Nrf2 and autophagy pathways. Scientific Reports 2016 6:1. 2016 Aug 24;6(1):1–12.
  • El-Sayed RM, Abdelaziz AM, Zaki HF, Abdel Rasheed NO. Cilostazol novel neuroprotective mechanism against rotenone-induced Parkinson’s disease in rats: Correlation between Nrf2 and HMGB1/TLR4/PI3K/Akt/mTOR signaling. Int Immunopharmacol. 2023 Apr 1; 117: 109986.
  • Peng K, Yang L, Wang J, Ye F, Dan G, Zhao Y, et al. The Interaction of Mitochondrial Biogenesis and Fission/Fusion Mediated by PGC-1α Regulates Rotenone-Induced Dopaminergic Neurotoxicity. Mol Neurobiol. 2017 Jul 1;54(5):3783–97.
  • Heo G, Sun MH, Jiang WJ, Li XH, Lee SH, Guo J, et al. Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes. PLoS One. 2022 Nov 1;17(11):e0277477.
  • Mutlu AG, Yıldız H. Civa Klorürün mtDNA’da Akut Etkisi. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2017 Oct 11;8(Ek 1):206–10.
  • Qin P, Chen X, Ma P, Li X, Lin Y, Liu X, et al. Mitochondrial DNA copy number and Alzheimer’s disease and Parkinson disease. Mitochondrion. 2025 Jul 1; 83: 102032.
  • Jo S, Oh JH, Lee EJ, Choi M, Lee J, Lee S, et al. Mitochondrial DNA Copy Number as a Potential Biomarker for the Severity of Motor Symptoms and Prognosis in Parkinson’s Disease. Movement Disorders. 2025 Mar 1;40(3):502–10.
  • Xia H, Wang ZH, Wang XB, Gao MR, Jiang S, Du XY, et al. Investigating the genetic association of mitochondrial DNA copy number with neurodegenerative diseases. BMC Neurol. 2025 Dec 1;25(1):1–9.
  • Ferreira AFF, Binda KH, Singulani MP, Pereira CPM, Ferrari GD, Alberici LC, et al. Physical exercise protects against mitochondria alterations in the 6-hidroxydopamine rat model of Parkinson’s disease. Behavioural Brain Research. 2020 Jun 1; 387: 112607.
  • Dong Y, Ren C, Tang H, Yan J, Zhang Y, Fan L, et al. Icariin promotes skeletal muscle mitochondrial biogenesis by the AMPK-PPARδ-PGC-1α signaling pathway: A potential mechanism for beneficial muscle function homeostasis. Food Biosci. 2024 Dec 1; 62: 105260.
  • Mikami T, Kim J, Park J, Lee H, Yaicharoen P, Suidasari S, et al. Olive leaf extract prevents obesity, cognitive decline, and depression and improves exercise capacity in mice. Scientific Reports 2021 11:1. 2021 Jun 14;11(1):1–14.
  • Erol Tınaztepe Ö. Sızma Zeytinyağı Fenoliklerinin Periferik Kan Mononükleer Hücrelerinin Mitokondriyal ve Nükleer DNA’sı Üzerindeki Etkisi. Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020 Aug 19
  • Demir S, Mentese A, Usta ZT, Alemdar NT, Demir EA, Aliyazicioglu Y. Alpha-pinene neutralizes cisplatin-induced reproductive toxicity in male rats through activation of Nrf2 pathway. Int Urol Nephrol. 2024 Feb 1;56(2):527–37.
  • Bouzenna H, Hfaiedh N, Giroux-Metges MA, Elfeki A, Talarmin H. Potential protective effects of alpha-pinene against cytotoxicity caused by aspirin in the IEC-6 cells. Biomed Pharmacother. 2017 Sep 1;93:961–8.
  • Rahimi K, Shirvani N, Sanaie P, Javadi A, Khademi M. The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats. Mol Biol Rep. 2023 Oct 1 [;50(10):8615–22.
  • Zhang B, Wang H, Yang Z, Cao M, Wang K, Wang G, et al. Protective effect of alpha-pinene against isoproterenol-induced myocardial infarction through NF-κB signaling pathway. Hum Exp Toxicol. 2020 Dec 1;39(12):1596–606.
  • de Mello NP, Berger MT, Lagerborg KA, Yan Y, Wettmarshausen J, Keipert S, et al. Pervasive glycative stress links metabolic imbalance and muscle atrophy in early-onset Parkinson’s disease. Mol Metab. 2025 Jul 1;97:102163.

Alpha-pinene protects soleus muscle and mitochondrial DNA in a rotenone-induced parkinson’s disease rat model

Year 2025, Volume: 64 Issue: 3, 589 - 597, 08.09.2025

Emel Kabartan Çökeli , Berna Tezcan Yavuz , Cansın Şirin , Gulay Hacıoğlu , Selma Cırrık

https://doi.org/10.19161/etd.1762616

Abstract

Aim: This study aims to evaluate the effects of alpha-pinene administration on the histological structure and mitochondrial DNA (mtDNA) copy number in the soleus muscle in a rotenone-induced Parkinson's disease (PD) rat model.
Materials and Methods: Adult male Sprague-Dawley rats were divided into control, rotenone (ROT), alpha-pinene (APN), and alpha-pinene + rotenone (APN+ROT) groups (n=12/group). ROT (2 mg/kg) and APN (50 mg/kg) were administered subcutaneously for 28 days. The histological structure of the soleus muscle was assessed using Hematoxylin-Eosin (H&E) staining. The mtDNA copy number was calculated as a ratio to nuclear DNA using quantitative polymerase chain reaction (qPCR).
Results: In the ROT group, atrophy, muscle fiber degeneration, and fibrosis were observed in the soleus muscle, along with a significant decrease in mtDNA copy number (p<0.01). APN treatment significantly reduced histological damage and mtDNA loss (p<0.05). In the APN+ROT group, the mtDNA copy number approached control levels (p>0.05), with significant histological improvements observed.
Conclusion: Alpha-pinene exhibits protective effects at both structural and molecular levels in the soleus muscle in a rotenone-induced PD model, suggesting its potential as a therapeutic agent against peripheral mitochondrial dysfunction.

Keywords

Alpha-pinene Rotenone Parkinson Soleus muscle mtDNA Histology

Ethical Statement

The study protocol received formal approval from the local animal ethics committee of the Giresun University (Ethical Approval No: 2025/6, date: 29.07.2025).

References

  • Chen C, Turnbull DM, Reeve AK. Mitochondrial Dysfunction in Parkinson’s Disease—Cause or Consequence? Biology 2019, Vol 8, Page 38.
  • Sherer TB, Kim JH, Betarbet R, Greenamyre JT. Subcutaneous Rotenone Exposure Causes Highly Selective Dopaminergic Degeneration and α-Synuclein Aggregation. Exp Neurol. 2003 Jan 1;179(1):9–16.
  • Panov A, Dikalov S, Shalbuyeva N, Taylor G, Sherer T, Greenamyre JT. Rotenone Model of Parkinson Disease. Journal of Biological Chemistry. 2005 Dec;280(51):42026–35.
  • Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, et al. Mitochondrial Complex I Inhibitor Rotenone Induces Apoptosis through Enhancing Mitochondrial Reactive Oxygen Species Production. Journal of Biological Chemistry. 2003 Mar;278(10):8516–25.
  • Aydınoğlu AT, Aksu Mengeş E, Balcı Hayta B. Serbest Mitokondriyal Dna nın Sistemik Dolaşımdaki Rolü Ve Hastalıkların Patogenezine Etkisi. Acibadem Universitesi Saglik Bilimleri Dergisi. 2020 Jan 1.
  • Musci R V., Fuqua JD, Peelor FF, Nguyen HVM, Richardson A, Choi S, et al. Age-induced changes in skeletal muscle mitochondrial DNA synthesis, quantity, and quality in genetically unique rats. Geroscience. 2025 Feb 1; 47(1):851–62.
  • Miller DM, Singh IN, Wang JA, Hall ED. Nrf2-ARE activator carnosic acid decreases mitochondrial dysfunction, oxidative damage and neuronal cytoskeletal degradation following traumatic brain injury in mice. Exp Neurol. 2015 Feb 1; 264:103–10.
  • Carling PJ, Cree LM, Chinnery PF. The implications of mitochondrial DNA copy number regulation during embryogenesis. Mitochondrion. 2011 Sep;11(5):686–92.
  • Filograna R, Mennuni M, Alsina D, Larsson NG. Mitochondrial DNA copy number in human disease: the more the better? FEBS Lett. 2021 Apr 1; 595(8):976–1002.
  • Hood DA, Memme JM, Oliveira AN, Triolo M. Maintenance of Skeletal Muscle Mitochondria in Health, Exercise, and Aging. Annu Rev Physiol. 2019 Feb 10;81(Volume 81, 2019):19–41.
  • Venkatesan D, Iyer M, Narayanasamy A, Gopalakrishnan AV, Vellingiri B. Plausible Role of Mitochondrial DNA Copy Number in Neurodegeneration—a Need for Therapeutic Approach in Parkinson’s Disease (PD). Molecular Neurobiology 2023 60:12. 2023 Jul 31;60(12):6992–7008.
  • Rahmani H, Moloudi MR, Hashemi P, Hassanzadeh K, Izadpanah E. Alpha-Pinene Alleviates Motor Activity in Animal Model of Huntington’s Disease via Enhancing Antioxidant Capacity. Neurochem Res. 2023 Jun 1;48(6):1775–82.
  • Cirrik S, Hacioglu G, Kabartan E, Yavuz BT, Tomruk CS. Can Alpha-Pinene Prevent Methotrexate-Induced Cardiac and Hepatic Damage? Physiol Res. 2024 ;73(4):621.
  • Hajizadeh Moghaddam A, Malekzadeh Estalkhi F, Khanjani Jelodar S, Ahmed Hasan T, Farhadi-Pahnedari S, Karimian M. Neuroprotective effects of alpha-pinene against behavioral deficits in ketamine-induced mice model of schizophrenia: Focusing on oxidative stress status. IBRO Neurosci Rep. 2024 Jun 1; 16:182–9.
  • Yeh Y, Liu T, Wang L, Lee H, Ting C, Lee W, et al. A standardized extract of Ginkgo biloba suppresses doxorubicin‐induced oxidative stress and p53‐mediated mitochondrial apoptosis in rat testes. Br J Pharmacol. 2009 Jan 24;156(1):48–61.
  • Schmidt S, Messner CJ, Gaiser C, Hämmerli C, Suter-Dick L. Methotrexate-Induced Liver Injury Is Associated with Oxidative Stress, Impaired Mitochondrial Respiration, and Endoplasmic Reticulum Stress In Vitro. Int J Mol Sci. 2022 Dec 1; 23(23):15116.
  • Zuzarte M, Sousa C, Alves-Silva J, Salgueiro L. Plant Monoterpenes and Essential Oils as Potential Anti-Ageing Agents: Insights from Preclinical Data. Biomedicines 2024, Vol 12, Page 365. 2024 Feb 4;12(2):365.
  • Tezcan Yavuz B, Kabartan Cokeli E, Sirin Tomruk C, Hacioglu G, Cirrik S, Tomruk C. Alpha Pinene Affects Intestinal Permeability and Protects the Gastrointestinal System Against Rotenone Toxicity via the Keap1/Nrf2 Pathway in Rats. Neurotoxicity Research 2025 43:4. 2025 Jul 28;43(4):1–12.
  • Kabartan E, Cirrik S, Hacioglu G, Tezcan Yavuz B, Telkoparan-Akillilar P, Sirin Tomruk C. Therapeutic potential of alpha-pinene in methotrexate-induced testicular toxicity: focus on apoptosis, oxidative stress, and inflammatory pathways. Naunyn Schmiedebergs Arch Pharmacol. 2025 Jul 9;1–11.
  • Zhang ZN, Zhang JS, Xiang J, Yu ZH, Zhang W, Cai M, et al. Subcutaneous rotenone rat model of Parkinson’s disease: Dose exploration study. Brain Res. 2017 Jan 15; 1655:104–13.
  • Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov A V., Greenamyre JT. Chronic systemic pesticide exposure reproduces features of Parkinson’s disease. Nature Neuroscience 2000 3:12. 2000;3(12):1301–6.
  • Siddiqui MA, Ahmad J, Farshori NN, Saquib Q, Jahan S, Kashyap MP, et al. Rotenone-induced oxidative stress and apoptosis in human liver HepG2 cells. Mol Cell Biochem. 2013 Dec 21;384(1–2):59–69.
  • Zhou Q, Chen B, Wang X, Wu L, Yang Y, Cheng X, et al. Sulforaphane protects against rotenone-induced neurotoxicity in vivo: Involvement of the mTOR, Nrf2 and autophagy pathways. Scientific Reports 2016 6:1. 2016 Aug 24;6(1):1–12.
  • El-Sayed RM, Abdelaziz AM, Zaki HF, Abdel Rasheed NO. Cilostazol novel neuroprotective mechanism against rotenone-induced Parkinson’s disease in rats: Correlation between Nrf2 and HMGB1/TLR4/PI3K/Akt/mTOR signaling. Int Immunopharmacol. 2023 Apr 1; 117: 109986.
  • Peng K, Yang L, Wang J, Ye F, Dan G, Zhao Y, et al. The Interaction of Mitochondrial Biogenesis and Fission/Fusion Mediated by PGC-1α Regulates Rotenone-Induced Dopaminergic Neurotoxicity. Mol Neurobiol. 2017 Jul 1;54(5):3783–97.
  • Heo G, Sun MH, Jiang WJ, Li XH, Lee SH, Guo J, et al. Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes. PLoS One. 2022 Nov 1;17(11):e0277477.
  • Mutlu AG, Yıldız H. Civa Klorürün mtDNA’da Akut Etkisi. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2017 Oct 11;8(Ek 1):206–10.
  • Qin P, Chen X, Ma P, Li X, Lin Y, Liu X, et al. Mitochondrial DNA copy number and Alzheimer’s disease and Parkinson disease. Mitochondrion. 2025 Jul 1; 83: 102032.
  • Jo S, Oh JH, Lee EJ, Choi M, Lee J, Lee S, et al. Mitochondrial DNA Copy Number as a Potential Biomarker for the Severity of Motor Symptoms and Prognosis in Parkinson’s Disease. Movement Disorders. 2025 Mar 1;40(3):502–10.
  • Xia H, Wang ZH, Wang XB, Gao MR, Jiang S, Du XY, et al. Investigating the genetic association of mitochondrial DNA copy number with neurodegenerative diseases. BMC Neurol. 2025 Dec 1;25(1):1–9.
  • Ferreira AFF, Binda KH, Singulani MP, Pereira CPM, Ferrari GD, Alberici LC, et al. Physical exercise protects against mitochondria alterations in the 6-hidroxydopamine rat model of Parkinson’s disease. Behavioural Brain Research. 2020 Jun 1; 387: 112607.
  • Dong Y, Ren C, Tang H, Yan J, Zhang Y, Fan L, et al. Icariin promotes skeletal muscle mitochondrial biogenesis by the AMPK-PPARδ-PGC-1α signaling pathway: A potential mechanism for beneficial muscle function homeostasis. Food Biosci. 2024 Dec 1; 62: 105260.
  • Mikami T, Kim J, Park J, Lee H, Yaicharoen P, Suidasari S, et al. Olive leaf extract prevents obesity, cognitive decline, and depression and improves exercise capacity in mice. Scientific Reports 2021 11:1. 2021 Jun 14;11(1):1–14.
  • Erol Tınaztepe Ö. Sızma Zeytinyağı Fenoliklerinin Periferik Kan Mononükleer Hücrelerinin Mitokondriyal ve Nükleer DNA’sı Üzerindeki Etkisi. Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020 Aug 19
  • Demir S, Mentese A, Usta ZT, Alemdar NT, Demir EA, Aliyazicioglu Y. Alpha-pinene neutralizes cisplatin-induced reproductive toxicity in male rats through activation of Nrf2 pathway. Int Urol Nephrol. 2024 Feb 1;56(2):527–37.
  • Bouzenna H, Hfaiedh N, Giroux-Metges MA, Elfeki A, Talarmin H. Potential protective effects of alpha-pinene against cytotoxicity caused by aspirin in the IEC-6 cells. Biomed Pharmacother. 2017 Sep 1;93:961–8.
  • Rahimi K, Shirvani N, Sanaie P, Javadi A, Khademi M. The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats. Mol Biol Rep. 2023 Oct 1 [;50(10):8615–22.
  • Zhang B, Wang H, Yang Z, Cao M, Wang K, Wang G, et al. Protective effect of alpha-pinene against isoproterenol-induced myocardial infarction through NF-κB signaling pathway. Hum Exp Toxicol. 2020 Dec 1;39(12):1596–606.
  • de Mello NP, Berger MT, Lagerborg KA, Yan Y, Wettmarshausen J, Keipert S, et al. Pervasive glycative stress links metabolic imbalance and muscle atrophy in early-onset Parkinson’s disease. Mol Metab. 2025 Jul 1;97:102163.
There are 39 citations in total.

Details

Primary Language Turkish
Subjects Neurology and Neuromuscular Diseases, Medical Physiology (Other)
Journal Section Research Articles
Authors

Emel Kabartan Çökeli 0000-0002-6289-2310

Berna Tezcan Yavuz 0000-0002-7410-9485

Cansın Şirin 0000-0002-4530-701X

Gulay Hacıoğlu 0000-0002-8528-2371

Selma Cırrık 0000-0001-8474-0185

Publication Date September 8, 2025
Submission Date August 12, 2025
Acceptance Date August 18, 2025
Published in Issue Year 2025 Volume: 64 Issue: 3

Cite

Vancouver Kabartan Çökeli E, Tezcan Yavuz B, Şirin C, Hacıoğlu G, Cırrık S. Alfa-pinen rotenonla oluşturulan parkinson hastalığı sıçan modelinde soleus kası ve mitokondriyal DNA’yı korur. EJM. 2025;64(3):589-97.
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