Antibody labeling kits vs conventional labeling: A comparative study in immunofluorescence of cell cultures

Kubilay Doğan Kılıç; Ayşegül Taşkıran Özyazıcı; Büşra Horuz

doi:10.19161/etd.1599016

EN TR

Antibody labeling kits vs conventional labeling: A comparative study in immunofluorescence of cell cultures

Abstract

Aim: This study aims to evaluate the comparative performance of antibody labeling kits and conventional antibody labeling methods regarding efficiency, accuracy, and practical usability in immunofluorescence applications on cell cultures. Materials and Methods: Both labeling methods were applied to identical cell culture samples. Labeling efficiency, fluorescence intensity, and antibody specificity were assessed using quantitative fluorescence microscopy. Practical aspects such as ease of use, time efficiency, and cost were also systematically evaluated. Results: Antibody labeling kits significantly reduced preparation time while increasing labeling efficiency, achieving comparable results to conventional methods in fluorescence intensity and antibody specificity. In sum, it is reported that it is higher ease of use for Antibody Labeling Kits. Conclusion: Antibody labeling kits offer a reliable and user-friendly alternative for immunofluorescence studies. By enhancing labeling efficiency and simplifying operational processes, these kits can accelerate research workflows and improve experimental outcomes in cell-based assays.

Keywords

Antikor etiketleme kitleri ile geleneksel etiketleme: Hücre kültürlerinde karşılaştırmalı immünofloresans bir çalışma

Öz

Amaç: Bu çalışma, antikor işaretleme kitleri ile geleneksel antikor işaretleme yöntemlerinin immün floresans uygulamalarında hücre kültürleri üzerindeki verimlilik, doğruluk ve pratik kullanılabilirlik açısından karşılaştırmalı performansını değerlendirmeyi amaçlamaktadır. Gereç ve Yöntem: Çalışmada her iki işaretleme yöntemi, aynı hücre kültürü örneklerine uygulanmıştır. İşaretleme verimliliği, floresans yoğunluğu ve antikor özgüllüğü, nicel floresans mikroskopisi ile değerlendirilmiştir. Ayrıca kullanım kolaylığı, zaman verimliliği ve maliyet gibi pratik unsurlar incelenmiştir. Bulgular: Antikor işaretleme kitleri, hazırlık süresini önemli ölçüde azaltırken işaretleme verimliliğini arttırmış ve floresans yoğunluğu ile antikor özgüllüğü açısından geleneksel yöntemlere eşdeğer sonuçlar sağlamıştır. Bütün değişkenler bir arada değerlendirildiğinde, antikor İşaretleme Kitlerinin kullanım kolaylığını daha yüksek olarak bildirmiştir. Sonuç: Antikor işaretleme kitleri, immün floresans çalışmaları için güvenilir ve kullanıcı dostu bir alternatif sunmaktadır. Etiketleme verimliliğini artırmaları ve operasyonel süreçleri basitleştirmeleri, bu kitlerin araştırma süreçlerini hızlandırarak hücre temelli deneylerde daha iyi sonuçlar elde edilmesini sağlayabileceğini göstermektedir.

Anahtar Kelimeler

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Histoloji ve Embriyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Kubilay Doğan Kılıç ^*
0000-0002-9484-0777
Türkiye

Ayşegül Taşkıran Özyazıcı
0000-0001-9780-6948
Türkiye

Büşra Horuz
0009-0009-6203-4111
Türkiye

Yayımlanma Tarihi

12 Mart 2025

Gönderilme Tarihi

10 Aralık 2024

Kabul Tarihi

6 Ocak 2025

Yayımlandığı Sayı

Yıl 1970 Cilt: 64 Sayı: 1

DOI

https://doi.org/10.19161/etd.1599016

IZ

https://izlik.org/JA34PY38DB

Kaynak Göster

RIS / Bibtex

Vancouver

1.Kubilay Doğan Kılıç, Ayşegül Taşkıran Özyazıcı, Büşra Horuz. Antibody labeling kits vs conventional labeling: A comparative study in immunofluorescence of cell cultures. ETD. 01 Mart 2025;64(1):118-27. doi:10.19161/etd.1599016