Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi

Şahin Öztürk; Burak Durmaz; Hikmet Memmedov; Latife Merve Oktay; Nur Selvi Günel; Murat Olukman; Eser Y. Sözmen

doi:10.19161/etd.887360

EN TR

Effect of ferulic acid on cytokine release in human leukemia monocytic cells induced with lipopolysaccharides

Abstract

Aim: Macrophages are accepted as cells that initially contact with the pathogens and initiate the innate immune response. They play effective roles in innate immune and inflammatory responses by intercellular relations and inflammatory mediator secretion. Human THP-1 leukemia monocytic cells are frequently used for the in vitro determination of the signal pathways, and the functions of macrophages. Lipopolysaccharide is commonly used to induce macrophage differentiation of monocytic cell lines but the extent of differentiation in comparison to primary tissue macrophages is unclear. Ferulic acid (FA) is known to inhibit the expression and / or activity of proinflammatory cytokines, including inducible nitric oxide synthase, caspases, and cyclooxygenase (COX)-2. In this study, we aimed to investigate the effect of Ferulic acid on cytokine (COX-1, IL-1α, IL-1β, TNF-α, IL-6, IL-10, NF-kβ and IFN-γ) levels in lipopolysaccharide induced THP-1 monocyte cells. Materials and Methods: TNF alpha levels were measured by performing applications at different concentrations and times, and the most appropriate concentration and duration were determined. Cytokine levels of COX-1, IL-1α, IL-1β, TNF-α, IL-6, IL-10, NF-kβ and IFN-γ were measured by ELISA in supernatants collected at the end of incubation times from wells. Results: We have found that ferulic acid inhibits the expression of TNF-α, IL-1α and IL-1β in LPS-induced macrophages by inhibition of NF-kB. Conclusion: It has been found that the effect of ferulic acid on cytokine release in LPS-induced THP-1 cells may be effective in the treatment of excessive inflammatory response and protection against auto-immune diseases.

Keywords

Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi

Öz

Amaç: Makrofajlar doğal immün cevabı başlatan ve patojenle ilk temasta bulunan hücreler olarak kabul edilmektedir. Hem hücreler arası ilişkiler hem de inflamatuar mediyatörlerin salınımı yoluyla doğal immün ve inflamatuar yanıtta etkin rol oynamaktadırlar. İnsan THP-1 lösemi monositik hücreleri, makrofajların in vitro olarak fonksiyonlarını, mekanizmalarını ve sinyal yollarını araştırmak için en çok kullanılan hücre dizisidir. Lipopolisakkarid (LPS) makrofaj farklılaşmasını başlatmak için yaygın olarak kullanılan uyarıcılar arasında yer almaktadır. Ferulik asid (FA)'in, indüklenebilir nitrik oksit sentaz, kaspazlar ve siklooksijenaz (COX)-2 dahil olmak üzere proinflamatuar sitokinlerin ekspresyonunu ve/veya aktivitesini inhibe ettiği bilinmektedir. Bu çalışmada, LPS ile indüklenen THP-1 monosit hücrelerinde Ferulik asidin sitokin (COX-1, IL-lα, IL-1β, TNF-α, IL-6, IL-10, NF-kβ ve IFN-y) düzeyleri üzerindeki etkisini araştırmayı amaçladık. Gereç ve Yöntem: TNF alfa seviyeleri farklı konsantrasyonlarda ve zamanlarda LPS eklenerek ölçüldü ve en uygun konsantrasyon ve süre belirlendi. COX-1, IL-lα, IL-lβ, TNF-α, IL-6, IL-10, NF-kβ ve IFN-y'nin sitokin miktarları, inkübasyon sürelerinin sonunda toplanan süpernatanlarda ELISA ile ölçüldü. Bulgular: Ferulik asidin, NF-kβ inhibisyonu ile LPS ile indüklenen THP-1 hücrelerinde artan TNF-α, IL-lα ve IL-lβ ekspresyonunu inhibe ettiğini bulduk. Sonuç: Ferulik asidin LPS ile indüklenen THP-1 hücrelerinde sitokin salınımı üzerindeki etkisinin gösterilmesi, aşırı inflamatuar yanıtın tedavisinde ve oto-immün hastalıklara karşı korunmada etkili olabileceğini düşündürdü

Anahtar Kelimeler

Destekleyen Kurum

EGE ÜNİVERSİTESİ BAP ( Bilimsel Araştırma Projesi)

Teşekkür

Bu çalışma EGE ÜNİVERSİTESİ BAP ( Bilimsel Araştırma Projesi) tarafından desteklenmiştir, bu projedeki destekleri için teşekkür ederiz (Proje 16-TIP-049). Yazarlar, teknik yardımlarından dolayı Ege Üniversitesi Çocuk Hastanesi Metabolizma Laboratuvarı'na çok teşekkür ederiz.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Şahin Öztürk
0000-0002-5793-4993
Türkiye

Burak Durmaz ^*
0000-0002-5285-3641
Türkiye

Hikmet Memmedov
0000-0002-8012-0445
Türkiye

Latife Merve Oktay
0000-0002-7110-3379
Türkiye

Nur Selvi Günel
0000-0003-0612-2263
Türkiye

Murat Olukman
0000-0002-9868-4716
Türkiye

Eser Y. Sözmen
0000-0002-6383-6724
Türkiye

Yayımlanma Tarihi

31 Mart 2021

Gönderilme Tarihi

20 Ekim 2020

Kabul Tarihi

30 Kasım 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 60 Sayı: 1

DOI

https://doi.org/10.19161/etd.887360

IZ

https://izlik.org/JA63SZ62PH

Kaynak Göster

RIS / Bibtex

Vancouver

1.Şahin Öztürk, Burak Durmaz, Hikmet Memmedov, Latife Merve Oktay, Nur Selvi Günel, Murat Olukman, Eser Y. Sözmen. Ferulik asitin lipopolisakkaridaz ile induklenmiş insan lösemi monositik hücrelerinde sitokin salınımına etkisi. ETD. 01 Mart 2021;60(1):39-50. doi:10.19161/etd.887360

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