Anti-inflammatory effects of Iturin A on the 6-OHDA-induced SH-SY5Y cell model of Parkinson’s disease

Pınar Altın Çelik; Muazzez Derya Andeden; Hamiyet Altuntaş

doi:10.19161/etd.1581447

EN TR

Anti-inflammatory effects of Iturin A on the 6-OHDA-induced SH-SY5Y cell model of Parkinson’s disease

Abstract

Aim: Biosurfactants produced by bacteria are particularly important due to their antibacterial, antifungal, and antiviral properties, which offer therapeutic and biomedical potential. Nevertheless, no research has examined the impact of Bacillus-derived biosurfactant Iturin A on Parkinson's disease. This study examined the neuroprotection through cytokine modulation of Iturin A to formulate a novel therapeutic approach for Parkinson's disease. Materials and Methods: The effect of Iturin A on cell viability in SH-SY5Y cells was evaluated by the [3-(4,5-Dimethyl thiazole-2) 2,5-diphenyltetrazolium bromide] method. As a result of this experiment, the non-toxic dose was determined by cell viability (%). To evaluate the neuroprotective effect of Iturin A, SH-SY5Y cells were treated with a non-toxic dose of Iturin A and then induced with 6-OHDA. After these treatments, cytokines (interleukin 6, transforming growth factor beta) and chemokine (interleukin 8) in SH-SY5Y cells were evaluated by ELISA kit. GraphPad Prism 9 was used to analyze all data. Results: Using one-way ANOVA and Dunn's post hoc test, normally distributed data was tested for multiple comparisons. Treatment with Iturin A for 48 hours showed a neuroprotective effect on 6-hydroxy dopamine-induced SH-SY5Y cells by statistically significantly decreasing the expression levels of interleukin 6 (P<0.001), transforming growth factor beta (P<0.001), and interleukin 8 (P<0.0001). Conclusion: Iturin A might offer new approaches to treating Parkinson's patients, and additional in vivo studies are necessary to confirm the in vitro results.

Keywords

İturin A'nın Parkinson hastalığının 6-OHDA kaynaklı SH-SY5Y hücre modelinde anti-inflamatuar etkileri

Öz

Amaç: Bakteriler tarafından üretilen biyosürfaktanlar, terapötik ve biyomedikal potansiyel sunan antibakteriyel, antifungal ve antiviral özellikleri nedeniyle özellikle önemlidir. Bununla birlikte, hiçbir araştırma Bacillus türevi biyosürfaktan İturin A'nın Parkinson hastalığı üzerindeki etkisini incelememiştir. Bu çalışma, Parkinson hastalığı için yeni bir terapötik yaklaşım formüle etmek amacıyla İturin A'nın sitokin aracılı nöroprotektif etkisini incelemiştir. Gereç ve Yöntem: İturin A'nın SH-SY5Y hücrelerindeki hücre canlılığı üzerindeki etkisi MTT [3-(4,5-Dimetil tiyazol-2) 2,5-difeniltetrazolium bromür] yöntemi ile değerlendirilmiştir. MTT deneyinin sonucunda, toksik olmayan doz hücre canlılığı (%) ile belirlendi. İturin A'nın nöroprotektif etkisini değerlendirmek için, SH-SY5Y hücreleri toksik olmayan bir doz Iturin A ile muamele edildi ve ardından 6-OHDA ile indüklendi. Bu muamelelerden sonra, SH-SY5Y hücrelerindeki sitokinler (IL-6, TGF-β) ve kemokin (IL-8) ELISA kiti ile değerlendirildi. Tüm verileri analiz etmek için GraphPad Prism 9 kullanıldı. Bulgular: İturin A ile 48 saatlik tedavi, sitokinlerin (IL-6, TGF-β) ve kemokin (IL-8) ifade seviyelerini değiştirerek 6-OHDA ile indüklenen SH-SY5Y hücrelerinde nöroprotektif bir etki gösterdi (p<0,05 - p<0,0001). Sonuç: Iturin A, Parkinson hastalarının tedavisinde yeni yaklaşımlar sunabilir ve in vitro sonuçları doğrulamak için ek in vivo çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Temel İmmünoloji

Bölüm

Araştırma Makalesi

Yazarlar

Pınar Altın Çelik ^*
0000-0001-8429-009X
Türkiye

Muazzez Derya Andeden
0000-0003-4390-5769
Türkiye

Hamiyet Altuntaş
0000-0001-6473-5813
Türkiye

Yayımlanma Tarihi

10 Haziran 2025

Gönderilme Tarihi

8 Kasım 2024

Kabul Tarihi

10 Ocak 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 64 Sayı: 2

DOI

https://doi.org/10.19161/etd.1581447

IZ

https://izlik.org/JA28ZS67EJ

Kaynak Göster

RIS / Bibtex

Vancouver

1.Pınar Altın Çelik, Muazzez Derya Andeden, Hamiyet Altuntaş. Anti-inflammatory effects of Iturin A on the 6-OHDA-induced SH-SY5Y cell model of Parkinson’s disease. ETD. 01 Haziran 2025;64(2):193-200. doi:10.19161/etd.1581447